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Exploration on Separation of Periplaneta Americana Small Peptides with Tricine-SDS-PAGE System 被引量:1
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作者 Shaohui WU Xiumei WU +2 位作者 Chenggui ZHANG guangmin dong guangming LIU 《Agricultural Biotechnology》 CAS 2012年第2期14-16,共3页
[ Objective] This study aimed to explore a Tricine-SDS-PAGE method for separation and purification of Periplaneta Americana small peptides. [ Method] By comparing the separation effects of three types of gels with dif... [ Objective] This study aimed to explore a Tricine-SDS-PAGE method for separation and purification of Periplaneta Americana small peptides. [ Method] By comparing the separation effects of three types of gels with different ratios on P. Americana small peptides, an appropriate Tricine-SDS-PAGE method for separation of small peptides was established. [ Result] Standard low molecular weight Marker was preliminarily separated to a certain extent with polyacrylamide gel electraphoresis, while the sample mixture was not separated due to the different levels of aggregation in separating gel, spacer gel and stacking gel. [Condusion] More appropriate polyacrylamide gel electrephoresis separation method or other better separation methods were to be further explored for separating peptide mixture with smaller molecular weight. 展开更多
关键词 Periplaneta Americana HPLC method SDS-PAGE
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