Revealing the crucial roles of suppressive immune microenvironment in cardiac myxoma progression

Cardiac myxoma is a commonly encountered tumor within the heart that has the potential to be life-threatening.However,the cellular composition of this condition is still not well understood.To fill this gap,we analyzed 75,641 cells from cardiac myxoma tissues based on single-cell sequencing.We defined a population of myxoma cells,which exhibited a resemblance to fibroblasts,yet they were distinguished by an increased expression of phosphodiesterases and genes associated with cell proliferation,differentiation,and adhesion.The clinical relevance of the cell populations indicated a higher proportion of myxoma cells and M2-like macrophage infiltration,along with their enhanced spatial interaction,were found to significantly contribute to the occurrence of embolism.The immune cells surrounding the myxoma exhibit inhibitory characteristics,with impaired function of T cells characterized by the expression of GZMK and TOX,along with a substantial infiltration of tumor-promoting macrophages expressed growth factors such as PDGFC.Furthermore,in vitro co-culture experiments showed that macrophages promoted the growth of myxoma cells significantly.In summary,this study presents a comprehensive single-cell atlas of cardiac myxoma,highlighting the heterogeneity of myxoma cells and their collaborative impact on immune cells.These findings shed light on the complex pathobiology of cardiac myxoma and present potential targets for intervention.

Physical and mechanical properties of hot-press sintering ternary CM_2A_8(CaMg_2Al_(16)O_(27))and C_2M_2A_(14)(Ca_2Mg_2Al_(28)O_(46))ceramics

The new ternary CM_2A_8(CaMg_2Al_(16)O_(27))and C_2M_2A_(14)(Ca_2Mg_2Al_(28)O_(46))pure and dense ceramics were first prepared by a hot-press sintering technique,and their physical and mechanical properties were investigated.The purity of obtained CM_2A_8 and C_2M_2A_(14) ceramics reaches 98.1 wt%and 97.5 wt%,respectively.Their microstructure is dense with few observable pores,and their grain size is about a few dozen microns.For their physical properties,the average apparent porosity of CM_2A_8 and C_2M_2A_(14) ceramics is 0.18% and 0.13%,and their average bulk density is 3.66 g/cm^3 and 3.71 g/cm^3,respectively.The relative density of CM_2A_8 ceramic is 98.12%and that of C_2M_2A_(14)ceramic is 98.67%.The thermal expansivity(50–1400℃)of CM_2A_8 and C_2M_2A_(14) ceramics is 9.24×10^(–6)K^(–1) and 8.92×10^(–6)K^(–1),respectively.The thermal conductivity of CM_2A_8 and C_2M_2A_(14) ceramic is 21.32 W/(m·K)and 23.25 W/(m·K)at 25℃and 18.76 W/(m·K)and 19.42 W/(m·K)as temperature rises to 350℃,respectively.In addition,the mechanical properties are also achieved.For CM_2A_8 ceramic,the flexural strength is 248 MPa,the fracture toughness is 2.17 MPa·m^(1/2),and the Vickers hardness is 12.26 GPa.For C_2M_2A_(14) ceramic,the flexural strength is 262 MPa,the fracture toughness is 2.23 MPa·m^(1/2),and the Vickers hardness is 12.95 GPa.

The Disc Theorem for the Schur Complement of Two Class Submatrices with γ-Diagonally Dominant Properties

The distribution for eigenvalues of Schur complement of matrices plays an important role in many mathematical problems.In this paper,we firstly present some criteria for H-matrix.Then as application,for two class matrices whose sub-matrices areγ-diagonally dominant and productγ-diagonally dominant,we show that the eigenvalues of the Schur complement are located in the Gersgorin discs and the Ostrowski discs of the original matrices under certain conditions.

Synergy of gut microbiota and host genome in driving heterosis expression of chickens

Heterosis has been widely utilized in agricultural production.Despite over a century of extensive research,the underlying mechanisms of heterosis remain elusive.Most hypotheses and research have focused on the genetic basis of heterosis.However,the potential role of gut microbiota in heterosis has been largely ignored.Here,we carefully design a crossbreeding experiment with two distinct broiler breeds and conduct 16S rRNA amplicon and transcriptome sequencing to investigate the synergistic role of gut microbiota and host genes in driving heterosis.We find that the breast muscle weight of hybrids exhibits a high heterosis,6.28%higher than the mid-parent value.A notable difference is observed in the composition and potential function of cecal microbiota between hybrids and their parents.Over 90%of differentially colonized microbiota and differentially expressed genes exhibit nonadditive patterns.Integrative analyses uncover associations between nonadditive genes and nonadditive microbiota,including a connection between the expression of cellular signaling pathways and metabolism-related genes and the abundance of Odor-ibacter,Oscillibacter,and Alistipes in hybrids.Moreover,higher abundances of these microbiota are related to better meat yield.In summary,these findings highlight the importance of gut microbiota in heterosis,serving as crucial factors that modulateheterosis expression in chickens.