This work aimed to reveal the expression profiles of tRNA-derived small RNAs(tsRNAs)in diabetes.Methods:Thirty-five diabetes patients and thirty-three controls were enrolled.The serum samples of 4 diabetes patients an...This work aimed to reveal the expression profiles of tRNA-derived small RNAs(tsRNAs)in diabetes.Methods:Thirty-five diabetes patients and thirty-three controls were enrolled.The serum samples of 4 diabetes patients and 4 controls were subjected to tRF and tiRNA polymerase chain reaction(PCR)Array analysis.Then quantitative PCR(qPCR)validation was performed on all the samples.Bioinformatics analyses were conducted to explore their functions.Results:We found 115 tsRNAs that significantly differed between the two groups.3′tiR-080-ProTGG(mt)was selected for further qPCR validation in all participants,and it was significantly decreased in diabetes patients compared with controls.Bioinformatics analysis indicated that 3′tiR-080-ProTGG(mt)may play regulatory roles via the cyclic adenosine monophosphate(cAMP)signaling pathway in the pathogenesis and progression of diabetes.Conclusion:Hence,we report that circulating tsRNAs are dysregulated that could be involved in the pathogenesis of diabetes.展开更多
The Upper Paleogene lacustrine fine-grained sandstones in the hinterlands of the northern Qaidam Basin mainly contain two sweet spot intervals.Fracture/fault,microfacies,petrology,pore features,diagenesis,etc.,were in...The Upper Paleogene lacustrine fine-grained sandstones in the hinterlands of the northern Qaidam Basin mainly contain two sweet spot intervals.Fracture/fault,microfacies,petrology,pore features,diagenesis,etc.,were innovatively combined to confirm the controlling factors on the reservoir quality of shallow delta-lacustrine fine-grained sandstones.The diagenesis of the original lake/surface/meteoric freshwater and acidic fluids related to the faults and unconformity occurred in an open geochemical system.Comprehensive analysis shows that the Upper Paleogene fine-grained sandstones were primarily formed in the early diagenetic B substage to the middle diagenetic A substage.Reservoir quality was controlled by fault systems,microfacies,burial-thermal history,diagenesis,hydrocarbon charging events(HCE),and abnormally high pressure.Shallow and deep double fault systems are the pathways for fluid flow and hydrocarbon migration.Sandstones developed in the high energy settings such as overwater(ODC)and underwater distributary channels(UDC)provide the material foundation for reservoirs.Moderate burial depth(3000-4000 m),moderate geothermal field(2.7-3.2℃/100 m),and late HCE(later than E3)represent the important factors to protect and improve pore volume.Meteoric freshwater with high concentrations of CO_(2)and organic acids from thermal decarboxylation are the main fluids leading to the dissolution and reformation of feldspar,rock fragments,calcite and anhydrite cements.Abnormally high pressure caused by the undercompaction in a large set of argillaceous rocks is the key to form high-quality reservoirs.Abnormal pressure zones reduced and inhibited the damage of compaction and quartz overgrowth to reservoir pores,allowing them to be better preserved.A reservoir quality evaluation model with bidirectional migration pathways,rich in clay minerals,poor in cements,superimposed dissolution and abnormally high pressure was proposed for the ODC/UDC finegrained sandstones.This model will facilitate the future development of fine-grained sandstone reservoirs both in the Upper Paleogene of the Qaidam Basin and elsewhere.展开更多
The global rise in sea level during the Late Cretaceous has been an issue under discussion by the international geological community. Despite the signifi- cance, its impact on the deposition of continental basins is n...The global rise in sea level during the Late Cretaceous has been an issue under discussion by the international geological community. Despite the signifi- cance, its impact on the deposition of continental basins is not well known. This paper presents the systematic review on stratigraphy and sedimentary facies compiled from 22 continental basins in northern Africa. The results indicate that the region was dominated by sediments of continental facies during Early Cretaceous, which were replaced by deposits of marine facies in Late Cretaceous. The spatio- temporal distribution of sedimentary facies suggests marine facies deposition reached as far south as Taou- deni-Iullemmeden-Chad-A1 Kufra-Upper Egypt basins during Turonian to Campanian. These results indicate that northern Africa underwent significant transgression during Late Cretaceous reaching its peak during Turonian to Coniacian. This significant transgression has been attributed to the global high sea-level during this time. Previous studies show that global rise in sea level in Late Cretaceous may have been driven by an increase in the volume of ocean water （attributed to high C02 concentra- tion and subsequently warm climate） and a decrease in the volume of the ocean basin （attributed to rapid production of oceanic crust and seamounts）. Tectonic mechanism of rapid production of oceanic crust and seamounts could play a fimdamental role in driving the global rise in sea level and subsequent transgression in northern Africa during Late Cretaceous.展开更多
基金funded by the Project of the Shanghai Municipal Health Commission(Grant No.20194Y0268)Project of Science and Technology Commission of Shanghai Municipality(Grant No.20Z11900400).
文摘This work aimed to reveal the expression profiles of tRNA-derived small RNAs(tsRNAs)in diabetes.Methods:Thirty-five diabetes patients and thirty-three controls were enrolled.The serum samples of 4 diabetes patients and 4 controls were subjected to tRF and tiRNA polymerase chain reaction(PCR)Array analysis.Then quantitative PCR(qPCR)validation was performed on all the samples.Bioinformatics analyses were conducted to explore their functions.Results:We found 115 tsRNAs that significantly differed between the two groups.3′tiR-080-ProTGG(mt)was selected for further qPCR validation in all participants,and it was significantly decreased in diabetes patients compared with controls.Bioinformatics analysis indicated that 3′tiR-080-ProTGG(mt)may play regulatory roles via the cyclic adenosine monophosphate(cAMP)signaling pathway in the pathogenesis and progression of diabetes.Conclusion:Hence,we report that circulating tsRNAs are dysregulated that could be involved in the pathogenesis of diabetes.
基金supported by the National Major Science and Technology Projects of China(No.2016ZX05033-001002)the National Natural Science Foundation of China(No.41272155)the China Scholarship Council。
文摘The Upper Paleogene lacustrine fine-grained sandstones in the hinterlands of the northern Qaidam Basin mainly contain two sweet spot intervals.Fracture/fault,microfacies,petrology,pore features,diagenesis,etc.,were innovatively combined to confirm the controlling factors on the reservoir quality of shallow delta-lacustrine fine-grained sandstones.The diagenesis of the original lake/surface/meteoric freshwater and acidic fluids related to the faults and unconformity occurred in an open geochemical system.Comprehensive analysis shows that the Upper Paleogene fine-grained sandstones were primarily formed in the early diagenetic B substage to the middle diagenetic A substage.Reservoir quality was controlled by fault systems,microfacies,burial-thermal history,diagenesis,hydrocarbon charging events(HCE),and abnormally high pressure.Shallow and deep double fault systems are the pathways for fluid flow and hydrocarbon migration.Sandstones developed in the high energy settings such as overwater(ODC)and underwater distributary channels(UDC)provide the material foundation for reservoirs.Moderate burial depth(3000-4000 m),moderate geothermal field(2.7-3.2℃/100 m),and late HCE(later than E3)represent the important factors to protect and improve pore volume.Meteoric freshwater with high concentrations of CO_(2)and organic acids from thermal decarboxylation are the main fluids leading to the dissolution and reformation of feldspar,rock fragments,calcite and anhydrite cements.Abnormally high pressure caused by the undercompaction in a large set of argillaceous rocks is the key to form high-quality reservoirs.Abnormal pressure zones reduced and inhibited the damage of compaction and quartz overgrowth to reservoir pores,allowing them to be better preserved.A reservoir quality evaluation model with bidirectional migration pathways,rich in clay minerals,poor in cements,superimposed dissolution and abnormally high pressure was proposed for the ODC/UDC finegrained sandstones.This model will facilitate the future development of fine-grained sandstone reservoirs both in the Upper Paleogene of the Qaidam Basin and elsewhere.
文摘The global rise in sea level during the Late Cretaceous has been an issue under discussion by the international geological community. Despite the signifi- cance, its impact on the deposition of continental basins is not well known. This paper presents the systematic review on stratigraphy and sedimentary facies compiled from 22 continental basins in northern Africa. The results indicate that the region was dominated by sediments of continental facies during Early Cretaceous, which were replaced by deposits of marine facies in Late Cretaceous. The spatio- temporal distribution of sedimentary facies suggests marine facies deposition reached as far south as Taou- deni-Iullemmeden-Chad-A1 Kufra-Upper Egypt basins during Turonian to Campanian. These results indicate that northern Africa underwent significant transgression during Late Cretaceous reaching its peak during Turonian to Coniacian. This significant transgression has been attributed to the global high sea-level during this time. Previous studies show that global rise in sea level in Late Cretaceous may have been driven by an increase in the volume of ocean water （attributed to high C02 concentra- tion and subsequently warm climate） and a decrease in the volume of the ocean basin （attributed to rapid production of oceanic crust and seamounts）. Tectonic mechanism of rapid production of oceanic crust and seamounts could play a fimdamental role in driving the global rise in sea level and subsequent transgression in northern Africa during Late Cretaceous.
