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Ballistic performance of additive manufacturing 316l stainless steel projectiles based on topology optimization method
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作者 Hao Xue Tao Wang +2 位作者 Xinyu Cui Yifan Wang guangyan huang 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第5期1-17,共17页
Material and structure made by additive manufacturing(AM)have received much attention lately due to their flexibility and ability to customize complex structures.This study first implements multiple objective topology... Material and structure made by additive manufacturing(AM)have received much attention lately due to their flexibility and ability to customize complex structures.This study first implements multiple objective topology optimization simulations based on a projectile perforation model,and a new topologic projectile is obtained.Then two types of 316L stainless steel projectiles(the solid and the topology)are printed in a selective laser melt(SLM)machine to evaluate the penetration performance of the projectiles by the ballistic test.The experiment results show that the dimensionless specific kinetic energy value of topologic projectiles is higher than that of solid projectiles,indicating the better penetration ability of the topologic projectiles.Finally,microscopic studies(scanning electron microscope and X-ray micro-CT)are performed on the remaining projectiles to investigate the failure mechanism of the internal structure of the topologic projectiles.An explicit dynamics simulation was also performed,and the failure locations of the residual topologic projectiles were in good agreement with the experimental results,which can better guide the design of new projectiles combining AM and topology optimization in the future. 展开更多
关键词 Additive manufacturing Topology optimization Ballistic performance Projectile design
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微生物源果胶酶在猪PK15细胞中异源表达及其酶学性质分析 被引量:4
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作者 莫健新 王豪强 +3 位作者 黄广燕 蔡更元 吴珍芳 张献伟 《遗传》 CAS CSCD 北大核心 2019年第8期736-745,共10页
果胶是植物细胞壁组分之一,是畜禽饲料中主要的抗营养因子,影响畜禽对日粮中能量和氮的利用效率。果胶酶在自然界中广泛存在于细菌、酵母和丝状真菌等微生物中,对解除果胶的抗营养作用、提高饲料利用率具有良好的效果。为了探索在猪细... 果胶是植物细胞壁组分之一,是畜禽饲料中主要的抗营养因子,影响畜禽对日粮中能量和氮的利用效率。果胶酶在自然界中广泛存在于细菌、酵母和丝状真菌等微生物中,对解除果胶的抗营养作用、提高饲料利用率具有良好的效果。为了探索在猪细胞中表达微生物源果胶酶基因的可行性,本研究通过脂质体转染法将微生物源果胶酶基因pg5a、pgI、pga3A和pgaA导入猪PK15细胞中进行异源表达,利用3,5-二硝基水杨酸(3,5-dinitrosalicylic acid,DNS)法测定这4种基因编码果胶酶的酶活力。结果显示,4种果胶酶基因均能在猪PK15细胞中转录出mRNA,但只有pg5a和pgI适应猪细胞表达系统。其中,果胶酶PG5A的最高酶活为0.95 U/mL,最适作用pH为pH4.0,在pH4.6~6.0范围内维持46%以上的酶活;PGI在pH5.0处获得最高酶活,为0.30 U/mL,在pH4.0~6.0范围内维持35%以上的酶活。消化蛋白酶耐受实验结果显示,PG5A和PGI对胃蛋白酶和胰蛋白酶均有较强的耐受能力,用1 mg/mL的猪胃蛋白酶处理2 h后,PG5A和PGI的剩余酶活分别为76%和71%;用1 mg/mL的猪胰蛋白酶处理2 h后,PG5A和PGI的剩余酶活分别为44%和93%。综上所述,果胶酶基因pg5a和pgI能在猪细胞中正常表达,其编码的果胶酶对猪消化道pH条件和消化蛋白酶具有较强的耐受能力,可作为制备转果胶酶基因猪的候选基因。 展开更多
关键词 果胶酶 果胶 抗营养因子 转基因
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MEK抑制剂PD0325901显著提高猪胎儿成纤维细胞ssODN介导的HDR效率
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作者 欧浩 李国玲 +5 位作者 王豪强 黄广燕 蔡更元 李紫聪 吴珍芳 张献伟 《遗传》 CAS CSCD 北大核心 2019年第4期327-336,共10页
基因组DNA发生双链断裂(double strand break, DSB)后主要通过同源定向修复(homologous-directed repair, HDR)和非同源末端连接(nonhomologous end joining, NHEJ)两种途径进行修复,其中单链寡聚核苷酸(single-stranded oligodeoxyribo... 基因组DNA发生双链断裂(double strand break, DSB)后主要通过同源定向修复(homologous-directed repair, HDR)和非同源末端连接(nonhomologous end joining, NHEJ)两种途径进行修复,其中单链寡聚核苷酸(single-stranded oligodeoxyribonucleotide, ssODN)介导的同源定向修复是动物基因组常用的基因组定点修饰技术,具有较大的科研和应用价值。为提高猪基因组ssODN介导HDR效率,本研究以猪胎儿成纤维细胞(porcine fetal fibroblasts, PFFs)为研究对象,利用丝裂原活化的细胞外信号调节激酶(mitogen-activated extracellular signal-regulated kinase, MEK)抑制剂PD0325901培养细胞,研究其对HDR效率的影响及作用分子机理。结果显示,PD0325901能显著提高PFFs的G_2期和S期细胞群百分比,减少G_1期细胞群比率,促进HDR修复因子的表达。在最适浓度250 nmol/L时,PD0325901使ssODN介导的GFP报告载体的修复效率提高了58.8%;同时使PFFs基因组位点DMD和ROSA26定点修饰效率分别提高了48.16%和17.64%。本研究表明,MEK抑制剂PD0325901能显著提高猪基因组ssODN介导的同源定向修复效率,为高效制备定点基因修饰动物模型提供了新思路。 展开更多
关键词 MEK抑制剂 同源定向修复(HDR) PD0325901 基因编辑
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A novel nest-based scheduling method for mobile wireless body area networks
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作者 Zhijun Xie guangyan huang +3 位作者 Roozbeh Zarei Zhenyan Ji Hongwu Ye Jing He 《Digital Communications and Networks》 SCIE 2020年第4期514-523,共10页
Wireless Body Area Networks(WBANs)comprise various sensors to monitor and collect various vital signals,such as blood pressure,pulse,heartbeat,body temperature,and blood sugar.A dense and mobile WBAN often suffers fro... Wireless Body Area Networks(WBANs)comprise various sensors to monitor and collect various vital signals,such as blood pressure,pulse,heartbeat,body temperature,and blood sugar.A dense and mobile WBAN often suffers from interference,which causes serious problems,such as wasting energy and degrading throughput.In reality,not all of the sensors in WBAN need to be active at the same time.Therefore,they can be divided into different groups so that each group works in turn to avoid interference.In this paper,a Nest-Based WBAN Scheduling(NBWS)algorithm is proposed to cluster sensors of the same types in a single or multiple WBANs into different groups to avoid interference.Particularly,we borrow the graph coloring theory to schedule all groups to work using a Time Division for Multimodal Sensor(TDMS)group scheduling model.Both theoretical analysis and experimental results demonstrate that the proposed NBWS algorithm performs better in terms of frequency of collisions,transmission delay,system throughput,and energy consumption compared to the counterpart methods. 展开更多
关键词 Interference elimination NEST SENSORS SCHEDULING Wireless body area networks Graph coloring theory
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An engineered xCas12i with high activity,high specificity,and broad PAM range
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作者 Hainan Zhang Xiangfeng Kong +20 位作者 Mingxing Xue Jing Hu Zikang Wang Yinghui Wei Haoqiang Wang Jingxing Zhou Weihong Zhang Mengqiu Xu Xiaowen Shen Fengcai Yin Zhiyuan Ai guangyan huang Junhui Xia Xueqiong Song Hengbin Li Yuan Yuan Jinhui Li Na Zhong Meiling Zhang Yingsi Zhou Hui Yang 《Protein & Cell》 SCIE CSCD 2023年第7期540-545,共6页
Dear Editor,The clustered regularly interspaced short palindromic repeats-Cas(CRISPR-Cas)systems,including type II Cas9 and type V Cas12 systems,which serve in the adaptive immunity of prokaryotes against viruses,have... Dear Editor,The clustered regularly interspaced short palindromic repeats-Cas(CRISPR-Cas)systems,including type II Cas9 and type V Cas12 systems,which serve in the adaptive immunity of prokaryotes against viruses,have been developed into genome-editing tools(Anzalone et al.,2020;Doudna,2020).Compared with type II systems,the type V systems including V-A to V-K showed more functional diversity(Yan et al.,2019).Amongst them,Cas12i has a relatively smaller size(1,033-1,093 aa),compared to SpCas9 and Cas12a,and has a 5'-TTN protospacer adjacent motif(PAM)preference(Yan et al.,2019). 展开更多
关键词 HIGH SPECIFICITY
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CRISPR/Cas13系统敲减HEK293T细胞ku70和lig4基因表达 被引量:1
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作者 王豪强 李国玲 +7 位作者 黄广燕 李紫聪 郑恩琴 徐铮 杨化强 吴珍芳 张献伟 刘德武 《生物工程学报》 CAS CSCD 北大核心 2020年第7期1414-1421,共8页
成簇的规律间隔的短回文重复序列(Clustered regularly interspaced short palindromic repeats,CRISPR)/CRISPR相关蛋白(CRISPR-associated proteins,Cas)系统是目前基因编辑、基因表达研究的热点,其中靶向RNA的CRISPR/Cas13系统的开发... 成簇的规律间隔的短回文重复序列(Clustered regularly interspaced short palindromic repeats,CRISPR)/CRISPR相关蛋白(CRISPR-associated proteins,Cas)系统是目前基因编辑、基因表达研究的热点,其中靶向RNA的CRISPR/Cas13系统的开发为RNA的干扰和编辑提供了新的方向。文中针对HEK293T细胞非同源末端连接(Nonhomologousendjoining,NHEJ)通路修复关键因子Ku70和Lig4的编码序列,设计并合成CRISPR/Cas13a、b系统相应的gRNA,检测其对ku70和lig4基因表达的影响。结果显示,Cas13a对ku70和lig4的RNA敲减效果可以达到50%以上,Cas13b对ku70和lig4的RNA敲减效果分别达到92%和76%;同时Cas13a、b系统能将Ku70和Lig4蛋白水平分别下调至68%和53%。CRISPR/Cas13系统可有效敲减HEK293T细胞RNA和蛋白质表达,为基因功能和调控研究提供一种新的策略。 展开更多
关键词 CRISPR/Cas13 KU70 lig4 基因敲减
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The microbuckling failure of Dyneema~? composites under compression
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作者 guangyan Li Wei Zhu guangyan huang 《Acta Mechanica Solida Sinica》 SCIE EI CSCD 2017年第4期425-434,共10页
Two grades of Dyneema composite laminates with the commercial designations of HB26 and HB50 were cut into blocks with or without an edge crack and compressed in the lon- gitudinal fiber direction. The cracked and uncr... Two grades of Dyneema composite laminates with the commercial designations of HB26 and HB50 were cut into blocks with or without an edge crack and compressed in the lon- gitudinal fiber direction. The cracked and uncracked specimens show similar compressive responses including failure pattern and failure load. The two grades of Dyneema composites exhibits different failure modes: a diffuse, sinusoidal buckling pattern for Dyneema HB50 due to its weak matrix constituent and a kink band for Dyneema~ HB26 due to its relatively stronger matrix constituent. An effective finite element model is used to simulate the collapse of Dyneema composites, and the sensitivity of laminate compressive responses to the overall effective shear modulus, interlaminar shear strength, thickness and imperfection angle are investigated. The change of failure mode from kink band to sinusoidal buckling pattern by decreasing the interlaminar shear strength is validated by the finite element analyses. 展开更多
关键词 Ultra-high molecular-weight polyethylene Microbuckling Cohesive Finite element analysis Compression
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