Plants can synthesize a wide range of terpenoids in response to various environmental cues.However,the specific regulatory mechanisms governing terpenoid biosynthesis at the cellular level remain largely elusive.In th...Plants can synthesize a wide range of terpenoids in response to various environmental cues.However,the specific regulatory mechanisms governing terpenoid biosynthesis at the cellular level remain largely elusive.In this study,we employed single-cell RNA sequencing to comprehensively characterize the transcriptome profile of cotton leaves and established a hierarchical transcriptional network regulating cellspecific terpenoid production.We observed substantial expression levels of genes associated with the biosynthesis of both volatile terpenes(such asβ-caryophyllene andβ-myrcene)and non-volatile gossypol-type terpenoids in secretory glandular cells.Moreover,two novel transcription factors,namely GoHSFA4a and GoNAC42,are identified to function downstream of the Gossypium PIGMENT GLAND FORMATION genes.Both transcription factors could directly regulate the expression of terpenoid biosynthetic genes in secretory glandular cells in response to developmental and environmental stimuli.For convenient retrieval of the single-cell RNA sequencing data generated in this study,we developed a user-friendly web server.Our findings not only offer valuable insights into the precise regulation of terpenoid biosynthesis genes in cotton leaves but also provide potential targets for cotton breeding endeavors.展开更多
基金the National Key R&D Program of China(2022YFF1001400)to J.-Q.H.the National Natural Science Foundation of China(32388201)to X.-Y.C.+5 种基金the Chinese Academy of Sciences(XDB27020207)to X.-Y.C.the Foundation of Youth Innovation Promotion Association of the Chinese Academy of Sciences to J.-Q.H.the Young Elite Scientists Sponsorship Program by CAST(2019QNRC001)to J.-Q.H.the Yunnan Revitalization Talent Support Program"Top Team"Project(202305AT350001)to X.-Y.C.Winall Hi-tech Seed Co.,Ltd.(GMLM2023)to X.-Y.C.the National Natural Science Foundation of China(32172041)to W.G.
文摘Plants can synthesize a wide range of terpenoids in response to various environmental cues.However,the specific regulatory mechanisms governing terpenoid biosynthesis at the cellular level remain largely elusive.In this study,we employed single-cell RNA sequencing to comprehensively characterize the transcriptome profile of cotton leaves and established a hierarchical transcriptional network regulating cellspecific terpenoid production.We observed substantial expression levels of genes associated with the biosynthesis of both volatile terpenes(such asβ-caryophyllene andβ-myrcene)and non-volatile gossypol-type terpenoids in secretory glandular cells.Moreover,two novel transcription factors,namely GoHSFA4a and GoNAC42,are identified to function downstream of the Gossypium PIGMENT GLAND FORMATION genes.Both transcription factors could directly regulate the expression of terpenoid biosynthetic genes in secretory glandular cells in response to developmental and environmental stimuli.For convenient retrieval of the single-cell RNA sequencing data generated in this study,we developed a user-friendly web server.Our findings not only offer valuable insights into the precise regulation of terpenoid biosynthesis genes in cotton leaves but also provide potential targets for cotton breeding endeavors.
