BACKGROUND Aberrant methylation is common during the initiation and progression of colorectal cancer(CRC),and detecting these changes that occur during early adenoma(ADE)formation and CRC progression has clinical valu...BACKGROUND Aberrant methylation is common during the initiation and progression of colorectal cancer(CRC),and detecting these changes that occur during early adenoma(ADE)formation and CRC progression has clinical value.AIM To identify potential DNA methylation markers specific to ADE and CRC.METHODS Here,we performed SeqCap targeted bisulfite sequencing and RNA-seq analysis of colorectal ADE and CRC samples to profile the epigenomic-transcriptomic landscape.RESULTS Comparing 22 CRC and 25 ADE samples,global methylation was higher in the former,but both showed similar methylation patterns regarding differentially methylated gene positions,chromatin signatures,and repeated elements.High-grade CRC tended to exhibit elevated methylation levels in gene promoter regions compared to those in low-grade CRC.Combined with RNA-seq gene expression data,we identified 14 methylation-regulated differentially expressed genes,of which only AGTR1 and NECAB1 methylation had prognostic significance.CONCLUSION Our results suggest that genome-wide alterations in DNA methylation occur during the early stages of CRC and demonstrate the methylation signatures associated with colorectal ADEs and CRC,suggesting prognostic biomarkers for CRC.展开更多
We describe a new species of the genus Hebius and provide evidence for the validity of H.septemlineatus comb.nov..Morphological and molecular analyses of Hebius specimens collected in Yunnan Province,China,revealed th...We describe a new species of the genus Hebius and provide evidence for the validity of H.septemlineatus comb.nov..Morphological and molecular analyses of Hebius specimens collected in Yunnan Province,China,revealed three distinct lineages,namely the newly described Hebius weixiensis sp.nov.,as well as H.octolineatus(Boulenger,1904),and H.septemlineatus comb.nov.(Schmidt 1925),which is removed from synonymy with H.octolineatus.Based on mitochondrial genealogy,Hebius weixiensis sp.nov.is sister to H.septemlineatus comb.nov.,while H.octolineatus is sister to H.bitaeniatus.The new species and H.septemlineatus comb.展开更多
基金the National Natural Science Foundation of China,No.81960504the“Xingdian Talents”Support Project of Yunnan Province,No.RLQB20200002+2 种基金the Medical Discipline Reserve Talents of Yunnan Province,No.H-2018015the Applied Basic Research Projects-Union Foundation of Kunming Medical University,No.2017FE467(-132)the Talent Introduction Project of Hubei Polytechnic University,No.21xjz34R。
文摘BACKGROUND Aberrant methylation is common during the initiation and progression of colorectal cancer(CRC),and detecting these changes that occur during early adenoma(ADE)formation and CRC progression has clinical value.AIM To identify potential DNA methylation markers specific to ADE and CRC.METHODS Here,we performed SeqCap targeted bisulfite sequencing and RNA-seq analysis of colorectal ADE and CRC samples to profile the epigenomic-transcriptomic landscape.RESULTS Comparing 22 CRC and 25 ADE samples,global methylation was higher in the former,but both showed similar methylation patterns regarding differentially methylated gene positions,chromatin signatures,and repeated elements.High-grade CRC tended to exhibit elevated methylation levels in gene promoter regions compared to those in low-grade CRC.Combined with RNA-seq gene expression data,we identified 14 methylation-regulated differentially expressed genes,of which only AGTR1 and NECAB1 methylation had prognostic significance.CONCLUSION Our results suggest that genome-wide alterations in DNA methylation occur during the early stages of CRC and demonstrate the methylation signatures associated with colorectal ADEs and CRC,suggesting prognostic biomarkers for CRC.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research(STEP)Program(2019QZKK0501)Biodiversity Survey and Assessment Project of the Ministry of Ecology and Environment,China(2019HJ2096001006)+4 种基金Strategic Priority Research Program of the Chinese Academy of Sciences(CAS)(XDA20050201)Digitalization,Development and Application of Biotic Resource(202002AA100007)Sciences and Technology Department of Sichuan Province(2020YFSY0033)State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology,CAS(GREKF 19-02)Animal Branch of the Germplasm Bank of Wild Species,CAS(Large Research Infrastructure Funding)。
文摘We describe a new species of the genus Hebius and provide evidence for the validity of H.septemlineatus comb.nov..Morphological and molecular analyses of Hebius specimens collected in Yunnan Province,China,revealed three distinct lineages,namely the newly described Hebius weixiensis sp.nov.,as well as H.octolineatus(Boulenger,1904),and H.septemlineatus comb.nov.(Schmidt 1925),which is removed from synonymy with H.octolineatus.Based on mitochondrial genealogy,Hebius weixiensis sp.nov.is sister to H.septemlineatus comb.nov.,while H.octolineatus is sister to H.bitaeniatus.The new species and H.septemlineatus comb.
