Present study aims at synthesizing a thermosensitive hydrogel for controlled release of insulin. According to a modified method, hydroxybutyl chitosan （HBC） hydrogel possessed thermal sensitivity is prepared which c...Present study aims at synthesizing a thermosensitive hydrogel for controlled release of insulin. According to a modified method, hydroxybutyl chitosan （HBC） hydrogel possessed thermal sensitivity is prepared which can form hydrogel at over 25℃. The HBC hydrogel is non-cytotoxic to mice fibroblasts cells （L929）. Insulin is 100% entrapped in the hydrogel, 38% of which is released in vitro from the concentration of 5% hydrogel after 48 h, whereas by enzymolysis with lysozyme, 80% of the total insulin is released after 48h. This study suggests that HBC hydrogel could be utilized for controlled release of insulin in a non-invasive manner.展开更多
With the quick emergence of antibiotic resistance and multi-drug resistant microbes,more and more attention has been paid to the development of new antimicrobial agents that have potential to take the challenge.Polysa...With the quick emergence of antibiotic resistance and multi-drug resistant microbes,more and more attention has been paid to the development of new antimicrobial agents that have potential to take the challenge.Polysaccharides,as one of the major classes of biopolymers,were explored for their antimicrobial properties and applications,owing to their easy accessibility,biocompatibility and easy modification.Polysaccharides and their derivatives have variable demonstrations and applications as antimicrobial agents and antimicrobial biomaterials.A variety of polysaccharides,such as chitosan,dextran,hyaluronic acid,cellulose,other plant/animal-derived polysaccharides and their derivatives have been explored for antimicrobial applications.We expect that this review can summarize the important progress of this field and inspire new concepts,which will contribute to the development of novel antimicrobial agents in combating antibiotic resistance and drug-resistant antimicrobial infections.展开更多
基金Acknowledgements This work was supported by a grant from International S＆T Cooperation Program of China （Grant No. 2013DFG32880）, the National Natural Science Foundation of China （Grant No. 31000423）, and the Doctoral Fund of Ministry of Education of China （Grant No. 20120132110012）
文摘Present study aims at synthesizing a thermosensitive hydrogel for controlled release of insulin. According to a modified method, hydroxybutyl chitosan （HBC） hydrogel possessed thermal sensitivity is prepared which can form hydrogel at over 25℃. The HBC hydrogel is non-cytotoxic to mice fibroblasts cells （L929）. Insulin is 100% entrapped in the hydrogel, 38% of which is released in vitro from the concentration of 5% hydrogel after 48 h, whereas by enzymolysis with lysozyme, 80% of the total insulin is released after 48h. This study suggests that HBC hydrogel could be utilized for controlled release of insulin in a non-invasive manner.
基金financially supported by the Natural Science Foundation of Shanghai(No.18ZR1410300)the National Natural Science Foundation of China(Nos.21861162010,21774031,and 31800801)+1 种基金the National Key Research and Development Program of China(No.2016YFC1100401)Research program of State Key Laboratory of Bioreactor Engineering,the Fundamental Research Funds for the Central Universities(Nos.22221818014 and 50321041917001)。
文摘With the quick emergence of antibiotic resistance and multi-drug resistant microbes,more and more attention has been paid to the development of new antimicrobial agents that have potential to take the challenge.Polysaccharides,as one of the major classes of biopolymers,were explored for their antimicrobial properties and applications,owing to their easy accessibility,biocompatibility and easy modification.Polysaccharides and their derivatives have variable demonstrations and applications as antimicrobial agents and antimicrobial biomaterials.A variety of polysaccharides,such as chitosan,dextran,hyaluronic acid,cellulose,other plant/animal-derived polysaccharides and their derivatives have been explored for antimicrobial applications.We expect that this review can summarize the important progress of this field and inspire new concepts,which will contribute to the development of novel antimicrobial agents in combating antibiotic resistance and drug-resistant antimicrobial infections.
