Two-dimensional polyacrylamide gel electrophoresis analysis of indomethacin-treated human colon cancer cells

AIM: To establish the two-dimensional gel electrophoresis (2-DE) profiles of indomethacin (IN)-treated human colon cancer cell line HCT116, and to provide a new way to study its anti-tumor molecular mechanism through analyzing a variety of protein maps.METHODS: Two-DE profiles of HCT116 were established in IN-treated and untreated groups. Total proteins were separated by immobilized pH gradient-based 2-DE. The gels were stained by silver, scanned by ImageScanner,and analyzed with Image Master software.RESULTS: Clear background, well-resolved and reproducible 2-DE patterns of HCT116 cells were acquired in IN-treated and untreated group. The average deviation of spot position was 0.896±0.177 mm in IEF direction and 1.106±0.289 mm in SDS-PAGE direction respectively. In IN-treated group,1 169±36 spots were detected and 1 061±32 spots were matched, the average matching rate was 90.6% in three gels. In untreated group, 1 256±50 spots were detected and 1 168±46 spots were matched, the average matching rate was 93.0% in three gels. Forty-five differential protein spots were displayed between IN-treated and untreated groups. Of which, 34 protein spots decreased and 9showed higher expression in IN-treated group, and only two protein spots showed an expression in untreated cells.CONCLUSION: Two-DE profiles of IN-treated and untreated HCT116 cells were established. Apparent 45 different protein spots were detected in IN-treated and untreated HCT116 cells. The analysis on differential protein spots may serve as a new way to study the molecule mechanism of IN-treated colon cancer.

A Multicenter Comparative Clinical Study of Sino-Levonorgestrel-Releasing Implants— No. I and No. II withNorplant

Objective To compare the efficacy, side effects and acceptability of two Sino-implants with Norplant Methods A randomized, prospective multicenter comparative clinical study was conducted in 10 clinical centers in China. Results Totally 1 001, 1 000 and 998 cases were recruited for Sino-implant No. I, No. II and Norplant, respectively, in 1993. The follow-up rate was 99.8% totally in 5 years. Three and five pregnancies occurred respectively in Sino-implant No. I and No. II group, while no pregnancy occurred in the Norplant group. The cumulative pregnancy rate was 0.4, 0.7 and 0 per 100 women respectively in implant No.I, No. II and Norplant group for five years, meaning that there was no statistical difference. There was no ectopic pregnancy in the three groups. The cumulative discontinuation rates at the end of five years were not significantly different among the three groups, either. Men- strual problems were the main reason for termination. The menstrual blood loss decreased significantly and no serious health problems arose fromimplants use. Conclusion The two Sino-implants provided similar high efficacy and safety to Norplant, therefore, they can be used by women at reproductive age who are from different areas,different of races, educational background and occupation.