Aedes (Stegomyia) albopictus, also known as the Asian tiger mosquito, is a mosquito which originated in Asia. In recent years, it has become increasingly rampant throughout the world. This mosquito can transmit severa...Aedes (Stegomyia) albopictus, also known as the Asian tiger mosquito, is a mosquito which originated in Asia. In recent years, it has become increasingly rampant throughout the world. This mosquito can transmit several arboviruses, including dengue, Zika and chikungunya viruses, and is considered a public health threat. Despite the urgent need of genome engineering to analyze specific gene functions, progress in genetical manipulation of Ae. albopictus has been slow due to a lack of efficient methods and genetic markers. In the present study, we established targeted disruptions in two genes, kynurenine hydroxylase (kh) and dopachrome conversion enzyme (yellow), to analyze the feasibility of generating visible phenotypes with genome editing by the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9) system in Ae. albopictus. Following Cas9 single guide RNA ribonucleoprotein injection into the posterior end of pre-blastoderm embryos, 30%-50% of fertile survivors produced alleles that failed to complement existing kh and yellow mutations. Complete eye and body pigmentation defects were readily observed in GI pupae and adults, indicating successful generation of highly heritable mutations. We conclude that the CRISPR/Cas9-mediated gene editing system can be used mAe. albopictus and that it can be adopted as an efficient tool for genome-scale analysis and biological study.展开更多
Background:Ethiopia is one of the African countries where Plasmodium falciparum and P.vivax co-exist.Monitoring and evaluation of current malaria transmission status is an important component of malaria control as it ...Background:Ethiopia is one of the African countries where Plasmodium falciparum and P.vivax co-exist.Monitoring and evaluation of current malaria transmission status is an important component of malaria control as it is a measure of the success of ongoing interventions and guides the planning of future control and elimination efforts.Main text:We evaluated changes in malaria control policy in Ethiopia,and reviewed dynamics of country-wide confirmed and clinical malaria cases by Plasmodium species and reported deaths for all ages and less than five years from 2001 to 2016.Districts level annual parasite incidence was analysed to characterize the malaria transmission stratification as implemented by the Ministry of Health.We found that Ethiopia has experienced major changes from 2003 to 2005 and subsequent adjustment in malaria diagnosis,treatment and vector control policy.Malaria interventions have been intensified represented by the increased insecticide treated net(ITN)and indoor residual spraying(IRS)coverage,improved health services and improved malaria diagnosis.However,countrywide ITN and IRS coverages were low,with 64%ITN coverage in 2016 and IRS coverage of 92.5%in 2016 and only implemented in epidemic-prone areas of>2500 m elevation.Clinical malaria incidence rate dropped from an average of 43.1 cases per 1000 population annually between 2001 and 2010 to 29.0 cases per 1000 population annually between 2011 and 2016.Malaria deaths decreased from 2.1 deaths per 100000 people annually between 2001 and 2010 to 1.1 deaths per 100000 people annually between 2011 to 2016.There was shrinkage in the malaria transmission map and high transmission is limited mainly to the western international border area.Proportion of P.falciparum malaria remained nearly unchanged from 2000 to 2016 indicating further efforts are needed to suppress transmission.Conclusions:Malaria morbidity and mortality have been significantly reduced in Ethiopia since 2001,however,malaria case incidence is still high,and there were major gaps between ITN ownership and compliance in malarious areas.Additional efforts are needed to target the high transmission area of western Ethiopia to sustain the achievements made to date.展开更多
基金the National Natural Science Foundation of China (81420108024,81528013)National Institute of Allergy and Infectious Diseases Grants of USA(AI136850)+2 种基金the National Key R&D Program of China(2016YFC1200500)the Natural Science Foundation of Guangdong Province(2014A030312016,2016A020251001)the Guangzhou Synergy Innovation Key Program for Health(201508020263,201605030010).
文摘Aedes (Stegomyia) albopictus, also known as the Asian tiger mosquito, is a mosquito which originated in Asia. In recent years, it has become increasingly rampant throughout the world. This mosquito can transmit several arboviruses, including dengue, Zika and chikungunya viruses, and is considered a public health threat. Despite the urgent need of genome engineering to analyze specific gene functions, progress in genetical manipulation of Ae. albopictus has been slow due to a lack of efficient methods and genetic markers. In the present study, we established targeted disruptions in two genes, kynurenine hydroxylase (kh) and dopachrome conversion enzyme (yellow), to analyze the feasibility of generating visible phenotypes with genome editing by the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9) system in Ae. albopictus. Following Cas9 single guide RNA ribonucleoprotein injection into the posterior end of pre-blastoderm embryos, 30%-50% of fertile survivors produced alleles that failed to complement existing kh and yellow mutations. Complete eye and body pigmentation defects were readily observed in GI pupae and adults, indicating successful generation of highly heritable mutations. We conclude that the CRISPR/Cas9-mediated gene editing system can be used mAe. albopictus and that it can be adopted as an efficient tool for genome-scale analysis and biological study.
基金This study was supported by grants from the National Institutes of Health(D43 TW001505,R01 A1050243 and U19 AI129326 to G.Yan).
文摘Background:Ethiopia is one of the African countries where Plasmodium falciparum and P.vivax co-exist.Monitoring and evaluation of current malaria transmission status is an important component of malaria control as it is a measure of the success of ongoing interventions and guides the planning of future control and elimination efforts.Main text:We evaluated changes in malaria control policy in Ethiopia,and reviewed dynamics of country-wide confirmed and clinical malaria cases by Plasmodium species and reported deaths for all ages and less than five years from 2001 to 2016.Districts level annual parasite incidence was analysed to characterize the malaria transmission stratification as implemented by the Ministry of Health.We found that Ethiopia has experienced major changes from 2003 to 2005 and subsequent adjustment in malaria diagnosis,treatment and vector control policy.Malaria interventions have been intensified represented by the increased insecticide treated net(ITN)and indoor residual spraying(IRS)coverage,improved health services and improved malaria diagnosis.However,countrywide ITN and IRS coverages were low,with 64%ITN coverage in 2016 and IRS coverage of 92.5%in 2016 and only implemented in epidemic-prone areas of>2500 m elevation.Clinical malaria incidence rate dropped from an average of 43.1 cases per 1000 population annually between 2001 and 2010 to 29.0 cases per 1000 population annually between 2011 and 2016.Malaria deaths decreased from 2.1 deaths per 100000 people annually between 2001 and 2010 to 1.1 deaths per 100000 people annually between 2011 to 2016.There was shrinkage in the malaria transmission map and high transmission is limited mainly to the western international border area.Proportion of P.falciparum malaria remained nearly unchanged from 2000 to 2016 indicating further efforts are needed to suppress transmission.Conclusions:Malaria morbidity and mortality have been significantly reduced in Ethiopia since 2001,however,malaria case incidence is still high,and there were major gaps between ITN ownership and compliance in malarious areas.Additional efforts are needed to target the high transmission area of western Ethiopia to sustain the achievements made to date.