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Establishment of Chlamydomonas reinhardtii chloroplast expression
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作者 Guochang Fan Ning Su +2 位作者 Zhonglin Zhang Xiangfu Wu guifang shen 《Chinese Science Bulletin》 SCIE EI CAS 1999年第18期1659-1664,共6页
An expression vector pACⅢ containing the chimeric gene HAV- VP3P1 and HCV-C gene has been constructed and transferred to C. reinhardtii by the biolistic method. The trans-formants have been identified by PCR, Souther... An expression vector pACⅢ containing the chimeric gene HAV- VP3P1 and HCV-C gene has been constructed and transferred to C. reinhardtii by the biolistic method. The trans-formants have been identified by PCR, Southern-blotting, Northern-blotting and Western-blotting assays after selecting on resistant medium and incubating in the dark. The results 展开更多
关键词 HAV-VP3P1 GENE HCV-C GENE C. REINHARDTII CHLOROPLAST EXPRESSION system HOMOLOGOUS recombinant.
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