A numerical study has been conducted to simulate the liquid/gas interface(meniscus) behaviors and capillary pressures in various capillary channels using the volume of fluid(VOF) method. Calculations are performed for...A numerical study has been conducted to simulate the liquid/gas interface(meniscus) behaviors and capillary pressures in various capillary channels using the volume of fluid(VOF) method. Calculations are performed for four channels whose cross-sectional shapes are circle, regular hexagon, square and equilateral triangle and for four solid/liquid contact angles of 30°, 60°, 120° and 150°. No calculation is needed for the contact angle of 90° because the liquid/gas interface in this case can be thought to be a plane surface. In the calculations, the liquid/gas interface in each channel is assumed to have a flat surface at the initial time, it changes towards its due shape thereafter, which is induced by the combined action of the surface tension and contact angle. After experiencing a period of damped oscillation, it stabilizes at a certain geometry. The interface dynamics and capillary pressures are compared among different channels under three categories including the equal inscribed circle radius, equal area, and equal circumscribed circle radius. The capillary pressure in the circular channel obtained from the simulation agrees well with that given by the Young–Laplace equation, supporting the reliability of the numerical model. The channels with equal inscribed circle radius yield the closest capillary pressures, while those with equal circumscribed circle radius give the most scattered capillary pressures,with those with equal area living in between. A correlation is developed to calculate the equivalent radius of a polygonal channel, which can be used to compute the capillary pressure in such a channel by combination with the Young–Laplace equation.展开更多
Many important crops(e.g.,tuber,root,and tree crops)are cross-pollinating.For these crops,no inbred lines are available for genetic study and breeding because they are self-incompatible,clonally propagated,or have a l...Many important crops(e.g.,tuber,root,and tree crops)are cross-pollinating.For these crops,no inbred lines are available for genetic study and breeding because they are self-incompatible,clonally propagated,or have a long generation time,making the identification of agronomically important genes difficult,particularly in crops with a complex autopolyploid genome.In this study,we developed a method,OutcrossSeq,for mapping agronomically important loci in outcrossing crops based on whole-genome low-coverage resequencing of a large genetic population,and designed three computation algorithms in OutcrossSeq for different types of outcrossing populations.We applied OutcrossSeq to a tuberous root crop(sweet potato,autopolyploid),a tree crop(walnut tree,highly heterozygous diploid),and hybrid crops(double-cross populations)to generate high-density genotype maps for the outcrossing populations,which enable precise identification of genomic loci underlying important agronomic traits.Candidate causative genes at these loci were detected based on functional clues.Taken together,our results indicate that OutcrossSeq is a robust and powerful method for identifying agronomically important genes in heterozygous species,including polyploids,in a cost-efficient way.The OutcrossSeq software and its instruction manual are available for downloading at www.xhhuanglab.cn/tool/OutcrossSeq.html.展开更多
基金Supported by the National Natural Science Foundation of China(51376103)
文摘A numerical study has been conducted to simulate the liquid/gas interface(meniscus) behaviors and capillary pressures in various capillary channels using the volume of fluid(VOF) method. Calculations are performed for four channels whose cross-sectional shapes are circle, regular hexagon, square and equilateral triangle and for four solid/liquid contact angles of 30°, 60°, 120° and 150°. No calculation is needed for the contact angle of 90° because the liquid/gas interface in this case can be thought to be a plane surface. In the calculations, the liquid/gas interface in each channel is assumed to have a flat surface at the initial time, it changes towards its due shape thereafter, which is induced by the combined action of the surface tension and contact angle. After experiencing a period of damped oscillation, it stabilizes at a certain geometry. The interface dynamics and capillary pressures are compared among different channels under three categories including the equal inscribed circle radius, equal area, and equal circumscribed circle radius. The capillary pressure in the circular channel obtained from the simulation agrees well with that given by the Young–Laplace equation, supporting the reliability of the numerical model. The channels with equal inscribed circle radius yield the closest capillary pressures, while those with equal circumscribed circle radius give the most scattered capillary pressures,with those with equal area living in between. A correlation is developed to calculate the equivalent radius of a polygonal channel, which can be used to compute the capillary pressure in such a channel by combination with the Young–Laplace equation.
基金We are grateful to Prof.Lars M.Steinmetz for the Tn5 plasmid.This work was funded by the Ministry of Science and Technology of China(2016YFD0100902 to X.H.,2018YFD1000701 and 2019YFD1000703 to J.Y.)the National Natural Science Foundation of China(31825015 to X.H.)+4 种基金the Program of Shanghai Academic Research Leader(18XD1402900 to X.H.)the Innovation Program of the Shanghai Municipal Education Commission(2017-01-07-00-02-E00039 to X.H.)the Shanghai Municipal Afforestation&City Appearance and Environmental Sanitation Administration(G182402,G192413,G192414,and G202402 to J.Y.)the Youth Innovation Promotion Association CAS(to J.Y.)the State Key Laboratory of Tree Genetics and Breeding support Program(CAFYBB2019ZY001 to D.P.).
文摘Many important crops(e.g.,tuber,root,and tree crops)are cross-pollinating.For these crops,no inbred lines are available for genetic study and breeding because they are self-incompatible,clonally propagated,or have a long generation time,making the identification of agronomically important genes difficult,particularly in crops with a complex autopolyploid genome.In this study,we developed a method,OutcrossSeq,for mapping agronomically important loci in outcrossing crops based on whole-genome low-coverage resequencing of a large genetic population,and designed three computation algorithms in OutcrossSeq for different types of outcrossing populations.We applied OutcrossSeq to a tuberous root crop(sweet potato,autopolyploid),a tree crop(walnut tree,highly heterozygous diploid),and hybrid crops(double-cross populations)to generate high-density genotype maps for the outcrossing populations,which enable precise identification of genomic loci underlying important agronomic traits.Candidate causative genes at these loci were detected based on functional clues.Taken together,our results indicate that OutcrossSeq is a robust and powerful method for identifying agronomically important genes in heterozygous species,including polyploids,in a cost-efficient way.The OutcrossSeq software and its instruction manual are available for downloading at www.xhhuanglab.cn/tool/OutcrossSeq.html.