Active Achilles tendon kinesitherapy facilitates the functional recovery of a ruptured Achilles tendon However, protein expression during the healing process remains a controversial issue. New Zealand rabbits, aged 14...Active Achilles tendon kinesitherapy facilitates the functional recovery of a ruptured Achilles tendon However, protein expression during the healing process remains a controversial issue. New Zealand rabbits, aged 14 weeks, underwent tenotomy followed immediately byAchilles tendon microsurgery to repair the Achilles tendon rupture. The tendon was then immobilized or subjected to postoperative early motion treatment （kinesitherapy）. Mass spectrography results showed that after 14 days of motion treatment, 18 protein spots were differentially expressed, among which, 12 were up-regulated, consisting of gelsolin isoform b and neurite growth-related protein collapsing response mediator protein 2. Western blot analysis showed that gelsolin isoform b was up-regulated at days 7-21 of motion treatment. These findings suggest that active Achilles tendon kinesitherapy promotes the neurite regeneration of a ruptured Achilles tendon and gelsolin isoform b can be used as a biomarker for Achilles tendon healing after kinesitherapy.展开更多
基金supported by the National Natural Science Foundation of China,No.30760256
文摘Active Achilles tendon kinesitherapy facilitates the functional recovery of a ruptured Achilles tendon However, protein expression during the healing process remains a controversial issue. New Zealand rabbits, aged 14 weeks, underwent tenotomy followed immediately byAchilles tendon microsurgery to repair the Achilles tendon rupture. The tendon was then immobilized or subjected to postoperative early motion treatment （kinesitherapy）. Mass spectrography results showed that after 14 days of motion treatment, 18 protein spots were differentially expressed, among which, 12 were up-regulated, consisting of gelsolin isoform b and neurite growth-related protein collapsing response mediator protein 2. Western blot analysis showed that gelsolin isoform b was up-regulated at days 7-21 of motion treatment. These findings suggest that active Achilles tendon kinesitherapy promotes the neurite regeneration of a ruptured Achilles tendon and gelsolin isoform b can be used as a biomarker for Achilles tendon healing after kinesitherapy.
基金This study was financially supported by the National Natural Science Foundation of China （No. 30760256）.Acknowledgements We extend many thanks to Yang Fanyuan, Jin Hong, Yang Fenyin and Zhou Xinwen in Centre of Proteomics and Systems Biology, Institutes of Biomedical Sciences, Fudan University, Shanghai, China. We also thank Zhang Yuanting, Zhang Binyin and Yin Xiulian in Institutes of Biomedical Sciences, Qinghua University, Beijing, China for their help in Western blotting analysis.
文摘跟腱的客观外科的修理(在) 破裂应该被活跃的腱动员立即跟随。动员什么时候应该开始的最佳的时间还是重要的争论。早 kinesitherapy 导致减少的康复时期。然而，进这个过程的详细机制的卓见没被获得。Proteomic 技术能被用来由与不同蛋白质的函数有关的微分表达式侧面分开并且净化蛋白质，但是在 proteomic 分析的区域研究在 3 点，在修理以后的天到目前为止没被学习。47 只新西兰白兔子被使随机化进 3 个组的方法。组 A (固定组， n=16 ) 收到的手术后的演员组固定；组 B (早运动组， n=16 ) 立即跟随修理的收到的早活跃的运动处理在从腱切断术另外一个的破裂， 15 只兔子担任了控制组(组 C ) 。AT 样品被准备 3 天列在后面 microsurgery。蛋白质被分开采用二维的 polyacrylamide 胶化电气泳动(2D 页) 。PDQuest 软件版本 8.0 被用来鉴别差别表示了蛋白质，由肽团指纹(PMF ) 和双人脚踏车团光谱分析列在后面，用生物工学信息(NCBI ) 蛋白质数据库检索然后为生物信息学分析的国家中心。446.33， 436.33 和 462.67 蛋白质吝啬的结果 A 在组在组 A，在组 B 的 14 只兔子和 13 只兔子在 13 只兔子的跟腱样品上看到 C 成功地被检测在 2D 页。分别地在组 A， B 和 C 有 40， 36 和 79 唯一的蛋白质。一些差别表示了蛋白质是有胶化的酶，帮助矩阵的 laser-desorption/ionization time-of-flight 团 spectrometry (MALDI-TOF MS ) 。我们成功地在组 A 和 B 识别了 9 和 11 不同蛋白质，例如 GAPDH， phosphoglycerate kinase 1， pro-alpha-1 类型 1 骨胶原， peroxiredoxin 1， alpha-1-antiproteinase E a-1 和 MAD2L1 绑定蛋白质，等等。并且有分子的女伴的一些，氧化应力，精力新陈代谢，信号 transduction，结合了腱房间表达式和蛋白质合成，增殖，区分并且仔细与 AT 有关正在愈合。GAPDH 蛋白质进一步通过西方的弄污被验证。一些差别表示了蛋白质，这被显示涉及各种各样的新陈代谢小径并且可以在起始的愈合起一个重要作用在破裂。在兔子在模型愈合的表示蛋白质可以贡献 3 天愈合的结论差别在通过新 mechanobiological 机制的破裂由于手术后的早 kinesitherapy 的申请。
