he potential benefits of endothelial cell seeding depend not only on effective cell attachment, but also on the ability of the cells to resist the shear stresses of blood flow. The shear stressresistance of cultured a...he potential benefits of endothelial cell seeding depend not only on effective cell attachment, but also on the ability of the cells to resist the shear stresses of blood flow. The shear stressresistance of cultured adult human endothelial cells was investigated on 6mm polytetrafluoroethylene vascular grafts (made in China). Endothelial cells were sodded onto pretreated vascular grafts at a high density. Grafts were then cultivated for 9 days to enable the maturation of thecytoskeleton, before they were exposed to pulsatile flow simulating the flow patterns and the wallshear forces of the small srtery. After 1 hour of per fusion,a cell loss of 11% in grafts pretreatedwith 20μ g/ ml fibronectin and of 12% in grafts pretreated with 5μ g /ml fibronectin coated human platelet- poor- plasma (p>0. 50 n = 10). Therefore we conclude that the endothelial celllining on vascular grafts pretreated with suitable substrates can form a shear stress-resistant endothelial cell monolayer on polytetrafluoroethylene vascular grafts.展开更多
文摘he potential benefits of endothelial cell seeding depend not only on effective cell attachment, but also on the ability of the cells to resist the shear stresses of blood flow. The shear stressresistance of cultured adult human endothelial cells was investigated on 6mm polytetrafluoroethylene vascular grafts (made in China). Endothelial cells were sodded onto pretreated vascular grafts at a high density. Grafts were then cultivated for 9 days to enable the maturation of thecytoskeleton, before they were exposed to pulsatile flow simulating the flow patterns and the wallshear forces of the small srtery. After 1 hour of per fusion,a cell loss of 11% in grafts pretreatedwith 20μ g/ ml fibronectin and of 12% in grafts pretreated with 5μ g /ml fibronectin coated human platelet- poor- plasma (p>0. 50 n = 10). Therefore we conclude that the endothelial celllining on vascular grafts pretreated with suitable substrates can form a shear stress-resistant endothelial cell monolayer on polytetrafluoroethylene vascular grafts.
