Objective: To observe the effect of herbal cake-partitioned moxibustion on liver X receptor alpha (LXRa) in rabbits with atherosclerosis. Methods: Thirty-six male New Zea la nd rabbits were ran domly divided into a no...Objective: To observe the effect of herbal cake-partitioned moxibustion on liver X receptor alpha (LXRa) in rabbits with atherosclerosis. Methods: Thirty-six male New Zea la nd rabbits were ran domly divided into a no rmal group, a model group, a herbal cake-partitioned moxibustion group and a simvastatin group according to the random number table method, with 9 rabbits in each group. Rabbits in the model group, the herbal cake-partitioned moxibustion group and the simvastatin group were modeled by high fat feeding method which took 12 weeks. After verification of the successful model, rabbits in the normal group were not treated, in the model group were bun died, in the herbal cake-partitioned moxibustio n group were treated with herbal cake-partitioned moxibustion, and those in the simvastatin group were treated with simvastatin, all for a total of 4 weeks. At the end of the experiment, the aorta and liver were observed for pathological changes;serum and liver were used to detect lipid levels;Western blot (WB) and real-time quantitative polymerase chain reaction (RT-qPCR) were used to detect LXRa protein and mRNA expression levels, respectively. Results: Compared with the normal group, the structure of aorta was disordered, the wall was rough and thick, the intima was unsmooth, and the vascular smooth muscle cells were arranged closely and disorderly in the model group, which was consistent with the characteristics of the rabbit model of aortic atherosclerosis. Compared with the model group, the aortic structure was clear;the degree of hepatocyte degeneration was reduced, the serum total cholesterol and low-density lipoprotein levels were significantly decreased (all P<0.01), the high-density lipoprotein level was elevated (P<0.01), and the total liver cholesterol was decreased significantly (P<0.01) in the rabbits of the herbal cake-partitioned moxibustion group and the simvastatin group;compared with the model group, the protein (PvO.Ol or P<0.05) and mRNA (P<0.01) expressions of rabbit LXRa in the herbal cake-partiti oned moxibusti on group and the simvastati n group were in creased. Conclusion: Herbal cake-partitioned moxibustion can improve the aortic and hepatic lesions, regulate blood lipid and liver lipid levels, in crease the expressi on of liver cholesterol reverse tran sport nu clear receptor LXRa, promote reverse cholesterol transport in the rabbits with aortic atherosclerosis, therefore produces an antiatherogenic effect.展开更多
文摘Objective: To observe the effect of herbal cake-partitioned moxibustion on liver X receptor alpha (LXRa) in rabbits with atherosclerosis. Methods: Thirty-six male New Zea la nd rabbits were ran domly divided into a no rmal group, a model group, a herbal cake-partitioned moxibustion group and a simvastatin group according to the random number table method, with 9 rabbits in each group. Rabbits in the model group, the herbal cake-partitioned moxibustion group and the simvastatin group were modeled by high fat feeding method which took 12 weeks. After verification of the successful model, rabbits in the normal group were not treated, in the model group were bun died, in the herbal cake-partitioned moxibustio n group were treated with herbal cake-partitioned moxibustion, and those in the simvastatin group were treated with simvastatin, all for a total of 4 weeks. At the end of the experiment, the aorta and liver were observed for pathological changes;serum and liver were used to detect lipid levels;Western blot (WB) and real-time quantitative polymerase chain reaction (RT-qPCR) were used to detect LXRa protein and mRNA expression levels, respectively. Results: Compared with the normal group, the structure of aorta was disordered, the wall was rough and thick, the intima was unsmooth, and the vascular smooth muscle cells were arranged closely and disorderly in the model group, which was consistent with the characteristics of the rabbit model of aortic atherosclerosis. Compared with the model group, the aortic structure was clear;the degree of hepatocyte degeneration was reduced, the serum total cholesterol and low-density lipoprotein levels were significantly decreased (all P<0.01), the high-density lipoprotein level was elevated (P<0.01), and the total liver cholesterol was decreased significantly (P<0.01) in the rabbits of the herbal cake-partitioned moxibustion group and the simvastatin group;compared with the model group, the protein (PvO.Ol or P<0.05) and mRNA (P<0.01) expressions of rabbit LXRa in the herbal cake-partiti oned moxibusti on group and the simvastati n group were in creased. Conclusion: Herbal cake-partitioned moxibustion can improve the aortic and hepatic lesions, regulate blood lipid and liver lipid levels, in crease the expressi on of liver cholesterol reverse tran sport nu clear receptor LXRa, promote reverse cholesterol transport in the rabbits with aortic atherosclerosis, therefore produces an antiatherogenic effect.
