Background:We explored the risk factors that affect the prognosis of patients with Paget disease of the breast(PD)and constructed a survival prediction model.Methods:The data of PD patients from 2004 to 2014 were coll...Background:We explored the risk factors that affect the prognosis of patients with Paget disease of the breast(PD)and constructed a survival prediction model.Methods:The data of PD patients from 2004 to 2014 were collected through the SEER database.The factors affecting the prognosis of PD patients were analysed by a single factor,and the prediction model of the independent risk factor transformation model diagram that affected their 1-,3-,and 5-year survival rates was screened by multivariate Cox proportional hazard regression analysis coefficients.The consistency index was used to evaluate its predictive value,and its predictive performance was tested by the 1000 bootstrap method.Then,the calibration curve was used to verify the predictive performance of the model,and the receiver operating characteristic curve and decision curve analysis were used to assess the sensitivity and practicability of the model compared to the American Cancer Commission staging(AJCC)staging system.Results:The consistency index of the model was 0.795(95%confidence interval 0.773–0.818).The calibration curve shows good consistency,while the receiver operating characteristic curve shows the sensitivity of the model to predict the survival rate of PD at 1,3,and 5 years.Decision curve analysis confirmed that the nomogram can predict the survival rate of PD patients at 1,3,and 5 years instead of the traditional AJCC system.Conclusion:Regarding the independent prognostic factors of age,marital status,AJCC classification,surgery,radiotherapy,chemotherapy and marital status conversion in PD patients,the nomogram established in sequence has higher accuracy and clinical value than the traditional AJCC system.展开更多
伴随着社会经济的快速发展,地铁、隧道、桥梁等建筑在人们的生活中占据的地位越来越高,预测分析建筑的结构变形数据,及时发现存在的安全隐患,至关重要.结合长短时记忆网络(Long Short Time Memory, LSTM)的优点,本文提出了一种基于双向...伴随着社会经济的快速发展,地铁、隧道、桥梁等建筑在人们的生活中占据的地位越来越高,预测分析建筑的结构变形数据,及时发现存在的安全隐患,至关重要.结合长短时记忆网络(Long Short Time Memory, LSTM)的优点,本文提出了一种基于双向长短时记忆网络(Bidirectional Long Short Time Memory, Bi-LSTM)的结构变形预测模型.该模型通过记忆时间节点前后的规律,预测当前节点变形数据,充分挖掘变形数据内部的关联信息.与WNN、LSTM、GRU模型进行对比,结果表明,该模型RMSE、MAPE、MAE分别下降了66.0%、61.2%、66.2%,是一种有效预测结构形变的方法.展开更多
The gap effect is a key factor in the design of the heat sealing in super- sonic vehicles subjected to an aerodynamic heat load. Built on S-A turbulence model and Roe discrete format, the aerodynamic environment aroun...The gap effect is a key factor in the design of the heat sealing in super- sonic vehicles subjected to an aerodynamic heat load. Built on S-A turbulence model and Roe discrete format, the aerodynamic environment around a gap on the surface of a supersonic aircraft was simulated by the finite volume method. As the presented results indicate, the gap effect depends not only on the attack angle, but also on the Mach number.展开更多
The global practical implementation of proton exchange membrane fuel cells(PEMFCs)heavily relies on the advancement of highly effective platinum(Pt)-based electrocatalysts for the oxygen reduction reaction(ORR).To ach...The global practical implementation of proton exchange membrane fuel cells(PEMFCs)heavily relies on the advancement of highly effective platinum(Pt)-based electrocatalysts for the oxygen reduction reaction(ORR).To achieve high ORR performance,electrocatalysts with highly accessible reactive surfaces are needed to promote the uncovering of active positions for easy mass transportation.In this critical review,we introduce different approaches for the emerging development of effective ORR electrocatalysts,which offer high activity and durability.The strategies,including morphological engineering,geometric configuration modification via supporting materials,alloys regulation,core-shell,and confinement engineering of single atom electrocatalysts(SAEs),are discussed in line with the goals and requirements of ORR performance enhancement.We review the ongoing development of Pt electrocatalysts based on the syntheses,nanoarchitecture,electrochemical performances,and stability.We eventually explore the obstacles and research directions on further developing more effective electrocatalysts.展开更多
The fracture behaviors and associated mechanisms of metallic materials under biaxial stress are vital for their manufacturability and service performance.In this work,the fracture behaviors of commercially pure titani...The fracture behaviors and associated mechanisms of metallic materials under biaxial stress are vital for their manufacturability and service performance.In this work,the fracture behaviors of commercially pure titanium(CP-Ti)under quasi-uniaxial and equi-biaxial tension were investigated by using the digital image correlation technique and finite element modeling.The fracture behaviors under quasi-uniaxial tension were characterized by a general normal fracture.In contrast,normal fracture firstly occurred per-pendicular to the rolling direction(RD)under equi-biaxial tension,followed by secondary shear fracture along the 45°direction relative to the RD.The normal fracture was attributed to the lower strain hard-ening ability in RD compared to the transverse direction(TD)induced by the TD-split type basal texture.The different hardening abilities introduced large shear stress in the 45°direction,which contributed sig-nificantly to the secondary shear fracture.An anisotropy parameter K(△S_(s)/σ_(s)),defined as the ratio of the equivalent effective traction stress to the yield strength,was proposed for the first time,to predict the fracture path with the impact of crystallographic preferred orientation.展开更多
The retinal pigment epithelium(RPE)and choroid are located behind the human retina and have multiple functions in the human visual system.Knowledge of the RPE and choroid cells and their gene expression profiles are f...The retinal pigment epithelium(RPE)and choroid are located behind the human retina and have multiple functions in the human visual system.Knowledge of the RPE and choroid cells and their gene expression profiles are fundamental for understanding retinal disease mechanisms and therapeutic strategies.Here,we sequenced the RNA of about 0.3 million single cells from human RPE and choroids across two regions and seven ages,revealing regional and age differences within the human RPE and choroid.Cell–cell interactions highlight the broad connectivity networks between the RPE and different choroid cell types.Moreover,the transcription factors and their target genes change during aging.The coding of somatic variations increases during aging in the human RPE and choroid at the single-cell level.Moreover,we identified ELN as a candidate for improving RPE degeneration and choroidal structure during aging.The mapping of the molecular architecture of the human RPE and choroid improves our understanding of the human vision support system and offers potential insights into the intervention targets for retinal diseases.展开更多
The human retina serves as a light detector and signals transmission tissue.Advanced insights into retinal disease mechanisms and therapeutic strategies require a deep understanding of healthy retina molecular events....The human retina serves as a light detector and signals transmission tissue.Advanced insights into retinal disease mechanisms and therapeutic strategies require a deep understanding of healthy retina molecular events.Here,we sequenced the m RNA of over 0.6 million single cells from human retinas across six regions at nine different ages.Sixty cell sub-types have been identified from the human mature retinas with unique markers.We revealed regional and age differences of gene expression profiles within the human retina.Cell-cell interaction analysis indicated a rich synaptic connection within the retinal cells.Gene expression regulon analysis revealed the specific expression of transcription factors and their regulated genes in human retina cell types.Some of the gene’s expression,such as DKK3,are elevated in aged retinas.A further functional investigation suggested that over expression of DKK3 could impact mitochondrial stability.Overall,decoding the molecular dynamic architecture of the human retina improves our understanding of the vision system.展开更多
N^(6)-methyladenosine(m^(6)A)modification,which is achieved by the METTL3/METTL14/WTAP methyltransferase complex,is the most abundant internal mRNA modification.Although recent evidence indicates that m^(6)A can regul...N^(6)-methyladenosine(m^(6)A)modification,which is achieved by the METTL3/METTL14/WTAP methyltransferase complex,is the most abundant internal mRNA modification.Although recent evidence indicates that m^(6)A can regulate neurodevelopment as well as synaptic function,the roles of m^(6)A modification in the cerebellum and related synaptic connections are not well established.Here,we report that Purkinje cell(PC)-specific WTAP knockout mice display early-onset ataxia concomitant with cerebellar atrophy due to extensive PC degeneration and apoptotic cell death.Loss of Wtap also causes the aberrant degradation of multiple PC synapses.WTAP depletion leads to decreased expression levels of METTL3/14 and reduced m^(6)A methylation in PCs.Moreover,the expression of GFAP and NF-L in the degenerating cerebellum is increased,suggesting severe neuronal injuries.In conclusion,this study demonstrates the critical role of WTAP-mediated m^(6)A modification in cerebellar PCs,thus providing unique insights related to neurodegenerative disorders.展开更多
To the Editor:Data from the Global Observatory on Donation and Transplantation (GODT) indicate that of the 126,670 solid organs reported to be transplanted in 2015, kidneys made up 66% of these organs.
基金This work was supported by The Guiding Project of Clinical Medical Technology Innovation in Hunan Province(2020SK51706)Clinical Research Center for Breast&Thyroid Disease Prevention in Hunan Province(2018SK4001)General guidance project of Hunan Provincial Health Commission(202204013843).
文摘Background:We explored the risk factors that affect the prognosis of patients with Paget disease of the breast(PD)and constructed a survival prediction model.Methods:The data of PD patients from 2004 to 2014 were collected through the SEER database.The factors affecting the prognosis of PD patients were analysed by a single factor,and the prediction model of the independent risk factor transformation model diagram that affected their 1-,3-,and 5-year survival rates was screened by multivariate Cox proportional hazard regression analysis coefficients.The consistency index was used to evaluate its predictive value,and its predictive performance was tested by the 1000 bootstrap method.Then,the calibration curve was used to verify the predictive performance of the model,and the receiver operating characteristic curve and decision curve analysis were used to assess the sensitivity and practicability of the model compared to the American Cancer Commission staging(AJCC)staging system.Results:The consistency index of the model was 0.795(95%confidence interval 0.773–0.818).The calibration curve shows good consistency,while the receiver operating characteristic curve shows the sensitivity of the model to predict the survival rate of PD at 1,3,and 5 years.Decision curve analysis confirmed that the nomogram can predict the survival rate of PD patients at 1,3,and 5 years instead of the traditional AJCC system.Conclusion:Regarding the independent prognostic factors of age,marital status,AJCC classification,surgery,radiotherapy,chemotherapy and marital status conversion in PD patients,the nomogram established in sequence has higher accuracy and clinical value than the traditional AJCC system.
文摘伴随着社会经济的快速发展,地铁、隧道、桥梁等建筑在人们的生活中占据的地位越来越高,预测分析建筑的结构变形数据,及时发现存在的安全隐患,至关重要.结合长短时记忆网络(Long Short Time Memory, LSTM)的优点,本文提出了一种基于双向长短时记忆网络(Bidirectional Long Short Time Memory, Bi-LSTM)的结构变形预测模型.该模型通过记忆时间节点前后的规律,预测当前节点变形数据,充分挖掘变形数据内部的关联信息.与WNN、LSTM、GRU模型进行对比,结果表明,该模型RMSE、MAPE、MAE分别下降了66.0%、61.2%、66.2%,是一种有效预测结构形变的方法.
基金supported by the National Natural Sciences Foundation of China(11272042)the Fundamental Research Funds for the Central Universities(2014YJS088)
文摘The gap effect is a key factor in the design of the heat sealing in super- sonic vehicles subjected to an aerodynamic heat load. Built on S-A turbulence model and Roe discrete format, the aerodynamic environment around a gap on the surface of a supersonic aircraft was simulated by the finite volume method. As the presented results indicate, the gap effect depends not only on the attack angle, but also on the Mach number.
基金A.A.,G.H.,S.H.,A.L.,and X.Y.J.thank the financial support from Kempe Foundation(SMK21-0011,SMK21-0020)A.L.acknowledges Swedish Research Council(2019-03865)European Union’s Horizon Europe research and innovation program under grant agreement No.101086667.X.Y.J.thanks the financial support from Horizon-EIC and Pathfinder challenges,Grant Number:101070976.
文摘The global practical implementation of proton exchange membrane fuel cells(PEMFCs)heavily relies on the advancement of highly effective platinum(Pt)-based electrocatalysts for the oxygen reduction reaction(ORR).To achieve high ORR performance,electrocatalysts with highly accessible reactive surfaces are needed to promote the uncovering of active positions for easy mass transportation.In this critical review,we introduce different approaches for the emerging development of effective ORR electrocatalysts,which offer high activity and durability.The strategies,including morphological engineering,geometric configuration modification via supporting materials,alloys regulation,core-shell,and confinement engineering of single atom electrocatalysts(SAEs),are discussed in line with the goals and requirements of ORR performance enhancement.We review the ongoing development of Pt electrocatalysts based on the syntheses,nanoarchitecture,electrochemical performances,and stability.We eventually explore the obstacles and research directions on further developing more effective electrocatalysts.
基金financially supported by the National Nat-ural Science Foundation of China(Nos.52101137,51971077,and U1737206)Shenzhen Fundamental Research Fund(Nos.JCYJ20210324122801005 and RCBS20210609103711035).
文摘The fracture behaviors and associated mechanisms of metallic materials under biaxial stress are vital for their manufacturability and service performance.In this work,the fracture behaviors of commercially pure titanium(CP-Ti)under quasi-uniaxial and equi-biaxial tension were investigated by using the digital image correlation technique and finite element modeling.The fracture behaviors under quasi-uniaxial tension were characterized by a general normal fracture.In contrast,normal fracture firstly occurred per-pendicular to the rolling direction(RD)under equi-biaxial tension,followed by secondary shear fracture along the 45°direction relative to the RD.The normal fracture was attributed to the lower strain hard-ening ability in RD compared to the transverse direction(TD)induced by the TD-split type basal texture.The different hardening abilities introduced large shear stress in the 45°direction,which contributed sig-nificantly to the secondary shear fracture.An anisotropy parameter K(△S_(s)/σ_(s)),defined as the ratio of the equivalent effective traction stress to the yield strength,was proposed for the first time,to predict the fracture path with the impact of crystallographic preferred orientation.
基金supported by the National Natural Science Foundation of China(No.81790643,81970839,82271105,and 82121003)the Sichuan Science and Technology Program(China)(No.2021YFS0033,2021YFS0404,2021YFS0369,and 2020ZYD037)the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(China)(No.2019-I2M-5-032).
文摘The retinal pigment epithelium(RPE)and choroid are located behind the human retina and have multiple functions in the human visual system.Knowledge of the RPE and choroid cells and their gene expression profiles are fundamental for understanding retinal disease mechanisms and therapeutic strategies.Here,we sequenced the RNA of about 0.3 million single cells from human RPE and choroids across two regions and seven ages,revealing regional and age differences within the human RPE and choroid.Cell–cell interactions highlight the broad connectivity networks between the RPE and different choroid cell types.Moreover,the transcription factors and their target genes change during aging.The coding of somatic variations increases during aging in the human RPE and choroid at the single-cell level.Moreover,we identified ELN as a candidate for improving RPE degeneration and choroidal structure during aging.The mapping of the molecular architecture of the human RPE and choroid improves our understanding of the human vision support system and offers potential insights into the intervention targets for retinal diseases.
基金supported by the National Natural Science Foundation of China(81790643,81970839,82271105,82121003)the Sichuan Science and Technology Program(2021YFS0033,2021YFS0369,2021YFS0404,2021JDGD0036)the Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(2019I2M-5-032)。
文摘The human retina serves as a light detector and signals transmission tissue.Advanced insights into retinal disease mechanisms and therapeutic strategies require a deep understanding of healthy retina molecular events.Here,we sequenced the m RNA of over 0.6 million single cells from human retinas across six regions at nine different ages.Sixty cell sub-types have been identified from the human mature retinas with unique markers.We revealed regional and age differences of gene expression profiles within the human retina.Cell-cell interaction analysis indicated a rich synaptic connection within the retinal cells.Gene expression regulon analysis revealed the specific expression of transcription factors and their regulated genes in human retina cell types.Some of the gene’s expression,such as DKK3,are elevated in aged retinas.A further functional investigation suggested that over expression of DKK3 could impact mitochondrial stability.Overall,decoding the molecular dynamic architecture of the human retina improves our understanding of the vision system.
基金supported by the National Natural Science Foundation of China(82121003,81970841,and 81790643)the Department of Science and Technology of Sichuan Province(2021YFS0386,2021YFS0369,20ZYD038,20ZYD037,2020JDZH0026,2021JDZH0022)+2 种基金the CAMS Innovation Fund for Medical Sciences(2019-12M-5-032)Huanhua Distingished Scholar grantthe Department of Chengdu Science and Technology(2021-YF05-01316-SN)。
文摘N^(6)-methyladenosine(m^(6)A)modification,which is achieved by the METTL3/METTL14/WTAP methyltransferase complex,is the most abundant internal mRNA modification.Although recent evidence indicates that m^(6)A can regulate neurodevelopment as well as synaptic function,the roles of m^(6)A modification in the cerebellum and related synaptic connections are not well established.Here,we report that Purkinje cell(PC)-specific WTAP knockout mice display early-onset ataxia concomitant with cerebellar atrophy due to extensive PC degeneration and apoptotic cell death.Loss of Wtap also causes the aberrant degradation of multiple PC synapses.WTAP depletion leads to decreased expression levels of METTL3/14 and reduced m^(6)A methylation in PCs.Moreover,the expression of GFAP and NF-L in the degenerating cerebellum is increased,suggesting severe neuronal injuries.In conclusion,this study demonstrates the critical role of WTAP-mediated m^(6)A modification in cerebellar PCs,thus providing unique insights related to neurodegenerative disorders.
文摘To the Editor:Data from the Global Observatory on Donation and Transplantation (GODT) indicate that of the 126,670 solid organs reported to be transplanted in 2015, kidneys made up 66% of these organs.
