[ Objective ] The study was to explore whether glutaredoxin (GRX2) and thioredoxin (TRX2) genes vary between Puerpiao chicken and Tengchongxue chicken in Yunnan Province. [ Methods ] Using Sanhuang chicken as the ...[ Objective ] The study was to explore whether glutaredoxin (GRX2) and thioredoxin (TRX2) genes vary between Puerpiao chicken and Tengchongxue chicken in Yunnan Province. [ Methods ] Using Sanhuang chicken as the control, the GRX2 and TRX2 genes were cloned from skirt tissues of Puerp/ao chicken and Tengchongxue chicken and then subjected to bioinformatics analysis. [ Results ] The sequencing results of both the GRX2 and TRX2 genes were completely consistent among Puerp/ao chicken, Tengchongxue chicken and Sanhuang chicken. And the sequences of GRX2 and TRX2 genes share a 100% homology with the results accessed in GenBank, and no any mutation was observed. [ Conclusion] The result provides scientific basis for investigating the antioxidant capacity of different chickens and utilizing elite local chicken resources.展开更多
基金Supported by Major Innovation Program for Extraction of Plant-derived NaturalDisease-resistant Components and Industrialization(KX140564)
文摘[ Objective ] The study was to explore whether glutaredoxin (GRX2) and thioredoxin (TRX2) genes vary between Puerpiao chicken and Tengchongxue chicken in Yunnan Province. [ Methods ] Using Sanhuang chicken as the control, the GRX2 and TRX2 genes were cloned from skirt tissues of Puerp/ao chicken and Tengchongxue chicken and then subjected to bioinformatics analysis. [ Results ] The sequencing results of both the GRX2 and TRX2 genes were completely consistent among Puerp/ao chicken, Tengchongxue chicken and Sanhuang chicken. And the sequences of GRX2 and TRX2 genes share a 100% homology with the results accessed in GenBank, and no any mutation was observed. [ Conclusion] The result provides scientific basis for investigating the antioxidant capacity of different chickens and utilizing elite local chicken resources.