Antioxidant Activity and Soothing Efficacy of Tambourissa Trichophylla Leaf Extract in Vitro Study

Present study is aimed to investigate in vitro biological efficacy of Tambourissa trichophylla leaf extract(TTLE),a plant-derived ingredient in cosmetic,on antioxidant activity and soothing efficacy.The total phenols content of TTLE was determined by the Folin-Ciocalteu method,antioxidant activity was tested by DPPH free radical scavenging assay,hyaluronidase inhibition activity was performed by using in vitro enzyme inhibitory assays based on spectrophotometric evaluation,and the soothing efficacy of TTLE was performed by the expression of nitric oxide(NO)and interleukin-6(IL-6)produced by LPS-induced RAW264.7 cells.The results shown that the total phenolic content of TTLE was as high as 54%,the DPPH scavenging activity was 81.18%at the concentration of 25μg/mL,the hyaluronidase inhibition activity was 69.04%at the concentration of 5 mg/mL,and the inhibition of NO and IL-6 were 15.70%and 76.57%at 0.05 mg/mL,respectively.TTLE has a high content of total phenols,high antioxidant activity and soothing effects,which indicated that TTLE was a promising ingredient in the development and application of cosmetics.

化妆品原料光甘草定的安全性评估

为评估化妆品原料光甘草定的安全性,为光甘草定在化妆品中的应用提供数据支持,按照《化妆品安全评估技术导则》(2021年版)对光甘草定用于化妆品的安全性进行了评估。结果显示,光甘草定在质量分数0.05%用量下的皮肤刺激性、眼刺激性、光毒性、光敏性、致敏性、遗传毒性、亚慢性毒性等风险较低,当使用的质量分数不高于0.05%时,用于无吸入暴露风险的驻留类面部、体用产品、唇膏及各种淋洗类产品中,应用风险可接受。在使用过程中应参考《中国药典》对原料中可能含有的有机溶剂残留进行质量控制,并在标签上增加必要的警示语,避免同时使用多种含有光甘草定的化妆品。

In Vitro Study on the Soothing and Oil Control Efficacy of P-Hydroxyacetophenone

p-Hydroxyacetophenone(p-HAP)was used as preservatives in cosmetics recently,their new efficacy was rarely reported.Here,We evaluate the soothing and oil control efficacy of p-HAP in vitro cells,providing a new efficacy basis for its application in cosmetics.The content of nitric oxide(NO),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)were determined in LPS-induced RAW264.7 and lipid contents were determined in testosterone-induced SZ95.The results revealed that p-HAP attenuated pro-inflammatory cytokines,such as NO,TNF-αand IL-6(P<0.01).Simultaneously,0.05mg/mL of p-HAP also inhibited the formation of lipid droplets in human sebocytes,with 17.64%of inhibition(P<0.001).These findings may be beneficial in the application of p-HAP as an efficacy ingredient in cosmetics,which may have a soothing and oil control efficacy according to the inhibition of pro-inflammatory cytokines and lipid contents.

Research of Several Evaluation Methods of Soothing Efficacy in Cosmetics

In order to search an appropriate evaluation method for the soothing effect of cosmetics,the LPS-induced macrophage model,SDS-stimulated zebrafish embryos model and the clinical assessment were used.The results showed that the concentration of NO and IL-6 was significantly reduced in LPS-induced RAW264.7 cells when applied to two essences.The inhibition rate of NO-release was 85.87%and 84.57%,while IL-6 was 44.18%and 38.76%,respectively.Furthermore,the spontaneous rotating of zebrafish embryos was significantly decreased to 51.85%and 76.54%in SDS-stimulated zebrafish embryos model.In the 28 days of clinical assessment,the concentration of hydration was significantly increased to 55.56%and 39.61%,while the erythrodesis was significantly decreased to 4.75%and 6.68%.This indicates that the three evaluation methods of soothing efficacy in this research were effective,and can be used in the evaluation of cosmetics.

烟酰胺腺嘌呤二核苷酸及其前体防治衰老相关疾病作用机制研究进展

烟酰胺腺嘌呤二核苷酸(nicotinamide adenine dinucleotide,NAD+)是多种脱氢酶的辅酶,在糖酵解、三羧酸循环等生理过程中发挥着重要作用,并参与能量代谢、蛋白质翻译和修饰、DNA修复和细胞应激等多种生物过程以对抗疾病。大量研究显示,衰老伴随着细胞和组织NAD+水平的下降,而这种下降与代谢性疾病、神经退行性疾病、免疫炎症、肿瘤等许多衰老相关疾病存在因果关系。NAD+及其前体(烟酸、烟酰胺单核苷酸、烟酰胺核糖等)在延缓衰老、防治许多衰老相关疾病等方面具有重要作用。本文主要综述了逆转NAD+年龄依赖性下降防治衰老相关疾病的调控机制,并简述了NAD+代谢、NAD+前体功能及有效补充NAD+的途径,为衰老相关疾病的预防和治疗方案提供参考。