AIM To compare the effects of liposomes andglyco-poly-L-lysine on liver targeted uptake andexpression of plasmid in rat liver.METHODS After binding with lipofectamine orgalactose-terminal glyco-poly-L-lysine,theplasmi...AIM To compare the effects of liposomes andglyco-poly-L-lysine on liver targeted uptake andexpression of plasmid in rat liver.METHODS After binding with lipofectamine orgalactose-terminal glyco-poly-L-lysine,theplasmid could be expressed in eukaryotic cellswhen injected into Wistar rats by intravenousroute.At different time intervals after the injection,the distribution and expression of the plasmid inliver of rats were observed and compared using insitu hybridization and immunohistochemistry.RESULTS The expression of the plasmid bindingto liposomes or G-PLL could be markedly observed24 h later,and began to decrease one week later,but it still could be observed up to three weeks.Both liposomes and G-PLL could deliver theplasmid to the liver effectively,but the effect of thelatter was better than the former concerning thedistribution and expression of the plasmid targeteduptake in the liver.CONCLUSION G-PLL is better than liposome asthe targeted carrier for delivering exogenous genesto the liver.展开更多
FURTHER development of the'doping'concept in the area of conductive polymers is based onthe following facts:polyaniline(PAn)doped by camphorsulfonic acid(CSA)and cast fromm-cresol solution has a conductivity 1...FURTHER development of the'doping'concept in the area of conductive polymers is based onthe following facts:polyaniline(PAn)doped by camphorsulfonic acid(CSA)and cast fromm-cresol solution has a conductivity 10~3 times higher than that cast from chloroform solution.The first metallic behavior of PAn cast from m-cresol was observed,i.e.conductivity decreas-展开更多
基金the National Natural Science Foundation of China(№39570336).
文摘AIM To compare the effects of liposomes andglyco-poly-L-lysine on liver targeted uptake andexpression of plasmid in rat liver.METHODS After binding with lipofectamine orgalactose-terminal glyco-poly-L-lysine,theplasmid could be expressed in eukaryotic cellswhen injected into Wistar rats by intravenousroute.At different time intervals after the injection,the distribution and expression of the plasmid inliver of rats were observed and compared using insitu hybridization and immunohistochemistry.RESULTS The expression of the plasmid bindingto liposomes or G-PLL could be markedly observed24 h later,and began to decrease one week later,but it still could be observed up to three weeks.Both liposomes and G-PLL could deliver theplasmid to the liver effectively,but the effect of thelatter was better than the former concerning thedistribution and expression of the plasmid targeteduptake in the liver.CONCLUSION G-PLL is better than liposome asthe targeted carrier for delivering exogenous genesto the liver.
文摘FURTHER development of the'doping'concept in the area of conductive polymers is based onthe following facts:polyaniline(PAn)doped by camphorsulfonic acid(CSA)and cast fromm-cresol solution has a conductivity 10~3 times higher than that cast from chloroform solution.The first metallic behavior of PAn cast from m-cresol was observed,i.e.conductivity decreas-
