Objective:The degree of liver cirrhosis is one of the most important diagnostic and prognostic assessments in chronic liver disease.Among the etiologies of liver cirrhosis,hepatitis B virus(HBV)infection-induced liver...Objective:The degree of liver cirrhosis is one of the most important diagnostic and prognostic assessments in chronic liver disease.Among the etiologies of liver cirrhosis,hepatitis B virus(HBV)infection-induced liver damage is most common in Asia-Pacific regions,particularly in China.Many current conventionally used preoperative estimation of liver cirrhosis models.展开更多
Objective:Currently,liver resection is the most effective curative treatment for patients with resectable hepatocellular carcinoma(HCC).Severe post-hepatectomy liver failure(PHLF)is a serious complication for HCC pati...Objective:Currently,liver resection is the most effective curative treatment for patients with resectable hepatocellular carcinoma(HCC).Severe post-hepatectomy liver failure(PHLF)is a serious complication for HCC patients undergoing liver resection.Models have been used to predict severe PHLF in patients with HCC,such as the Child-Pugh score,model for end-stage liver disease(MELD)score.展开更多
Objective: To construct a HepG2 cell line which stably expressing Hepatitis B e antigen(HBeAg)and investigate the effects of HBeAg on the proliferation,migration and invasion of HepG2 cells. Methods: The lentivirus ca...Objective: To construct a HepG2 cell line which stably expressing Hepatitis B e antigen(HBeAg)and investigate the effects of HBeAg on the proliferation,migration and invasion of HepG2 cells. Methods: The lentivirus carrying HBeAg gene was constructed and packaged. HepG2 cells were infected with the lentivirus and screened with puromycin to obtain HepG2 cells which stably expressing HBeAg(HepG2-HBeAg cells).The expression levels of HBeAg mRNA and protein were detected by RT-qPCR and Western blot,respectively. The content of HBeAg secretion in cell supernatant in both HepG2-HBeAg cells and control cells(HepG2-NC cells and HepG2 cells)were detected by IFMA assay. Furthermore,CCK-8 proliferation assay,colony formation assay and transwell migration and invasion assays were conducted to compare the abilities of cell proliferation,migration and invasion,respectively. Results: The expression of HBeAg in the HepG2-HBeAg cell was significantly higher than those in HepG2 cells and HepG2-NC cells. Secreted HBeAg in the supernatant of HepG2-HBeAg cells was 26. 33±2. 13 PEIU/mL but was undetectable in supernatant of control cells. The proliferation,migration and invasion were all significantly lower in HepG2-HBeAg cells compared to control cells(P<0. 01). Conclusion: A HepG2 cell line which stably expressing HBeAg was constructed successfully,and the over-expression of HBeAg could attenuate the proliferation,migration and invasion of hepatocellular carcinoma cells.展开更多
文摘Objective:The degree of liver cirrhosis is one of the most important diagnostic and prognostic assessments in chronic liver disease.Among the etiologies of liver cirrhosis,hepatitis B virus(HBV)infection-induced liver damage is most common in Asia-Pacific regions,particularly in China.Many current conventionally used preoperative estimation of liver cirrhosis models.
文摘Objective:Currently,liver resection is the most effective curative treatment for patients with resectable hepatocellular carcinoma(HCC).Severe post-hepatectomy liver failure(PHLF)is a serious complication for HCC patients undergoing liver resection.Models have been used to predict severe PHLF in patients with HCC,such as the Child-Pugh score,model for end-stage liver disease(MELD)score.
基金supported by the National Natural Science Foundation of China(No.81360315)Guangxi Medical and Health Technology Development and Application Project(No.S201629)
文摘Objective: To construct a HepG2 cell line which stably expressing Hepatitis B e antigen(HBeAg)and investigate the effects of HBeAg on the proliferation,migration and invasion of HepG2 cells. Methods: The lentivirus carrying HBeAg gene was constructed and packaged. HepG2 cells were infected with the lentivirus and screened with puromycin to obtain HepG2 cells which stably expressing HBeAg(HepG2-HBeAg cells).The expression levels of HBeAg mRNA and protein were detected by RT-qPCR and Western blot,respectively. The content of HBeAg secretion in cell supernatant in both HepG2-HBeAg cells and control cells(HepG2-NC cells and HepG2 cells)were detected by IFMA assay. Furthermore,CCK-8 proliferation assay,colony formation assay and transwell migration and invasion assays were conducted to compare the abilities of cell proliferation,migration and invasion,respectively. Results: The expression of HBeAg in the HepG2-HBeAg cell was significantly higher than those in HepG2 cells and HepG2-NC cells. Secreted HBeAg in the supernatant of HepG2-HBeAg cells was 26. 33±2. 13 PEIU/mL but was undetectable in supernatant of control cells. The proliferation,migration and invasion were all significantly lower in HepG2-HBeAg cells compared to control cells(P<0. 01). Conclusion: A HepG2 cell line which stably expressing HBeAg was constructed successfully,and the over-expression of HBeAg could attenuate the proliferation,migration and invasion of hepatocellular carcinoma cells.
