To the Editor:Chronic kidney disease(CKD)is a global burden of the public health.The global prevalence of CKD exceeded 10%while the awareness was around 10%.[1]In the era of big data,improving the identification of CK...To the Editor:Chronic kidney disease(CKD)is a global burden of the public health.The global prevalence of CKD exceeded 10%while the awareness was around 10%.[1]In the era of big data,improving the identification of CKD using informatic tools is important.Computable phenotype is proven as an efficient tool to facilitate the process of patient identification using electronic health record(EHR)data.展开更多
Microtus fortis is the only mammalian host that exhibits intrinsic resistance against Schistosoma japonicum infection.However,the underlying molecular mechanisms of this resistance are not yet known.Here,we perform th...Microtus fortis is the only mammalian host that exhibits intrinsic resistance against Schistosoma japonicum infection.However,the underlying molecular mechanisms of this resistance are not yet known.Here,we perform the first de novo genome assembly of M.fortis,comprehensive gene annotation analysis,and evolution analysis.Furthermore,we compare the recovery rate of schistosomes,pathological changes,and liver transcriptomes between M.fortis and mice at different time points after infection.We observe that the time and type of immune response in M.fortis are different from those in mice.M.fortis activates immune and inflammatory responses on the 10th day post infection,such as leukocyte extravasation,antibody activation,Fc-gamma receptor-mediated phagocytosis,and the interferon signaling cascade,which play important roles in preventing the development of schistosomes.In contrast,an intense immune response occurrs in mice at the late stages of infection and could not eliminate schistosomes.Infected mice suffer severe pathological injury and continuous decreases in cell cycle,lipid metabolism,and other functions.Our findings offer new insights into the intrinsic resistance mechanism of M.fortis against schistosome infection.The genome sequence also provides the basis for future studies of other important traits in M.fortis.展开更多
Dear Editor,Recent studies show that induced pluripotent stem cells(iPSCs)generated through ectopic expression of transcription factors retain an epigenetic memory of their original somatic cells(Kim et al.,2010;Polo ...Dear Editor,Recent studies show that induced pluripotent stem cells(iPSCs)generated through ectopic expression of transcription factors retain an epigenetic memory of their original somatic cells(Kim et al.,2010;Polo et al.,2010)or aberrant silencing of a single imprinted gene cluster(Liu et al.,2010;Stadtfeld et al.,2010),which affects their developmental and differentiation potentials.展开更多
基金National Natural Science Foundation of China(Nos.82100741,82003529,91846101,81771938,81900665,82090021)Beijing Municipal Science and Technology Commission(Grant No.7212201)+5 种基金the University of Michigan Health System-Peking University Health Science Center Joint Institute for Translational and Clinical Research(Nos.BMU2020JI011,BMU2019JI005,BMU2018JI012)Beijing Nova Programme Interdisciplinary Cooperation Project(No.Z191100001119008)National Key R&D Program of the Ministry of Science and Technology of China(No.2019YFC2005000)the National Key Research and Development Program of China(No.2018AAA0102100)PKU-Baidu Fund(Nos.2020BD005,2019BD017)CAMS Innovation Fund for Medical Sciences(No.2019-I2M-5-046)
文摘To the Editor:Chronic kidney disease(CKD)is a global burden of the public health.The global prevalence of CKD exceeded 10%while the awareness was around 10%.[1]In the era of big data,improving the identification of CKD using informatic tools is important.Computable phenotype is proven as an efficient tool to facilitate the process of patient identification using electronic health record(EHR)data.
基金This work was supported by the Key Project in the National Science&Technology Pillar Program from the Ministry of Science and Technology(2015BAI09B04)the National Natural Science Foundation of China(31872256,31472188)+2 种基金the National Key Research and Development Program of China(2017YFD0501306)the Science and Technology Service Network Initiative of Chinese Academy of Sciences(KFJ-STS-QYZD-126,ZDBS-SSW-DQC-02)CAS Youth Innovation Promotion Association,and SA-SIBS Scholarship Program.
文摘Microtus fortis is the only mammalian host that exhibits intrinsic resistance against Schistosoma japonicum infection.However,the underlying molecular mechanisms of this resistance are not yet known.Here,we perform the first de novo genome assembly of M.fortis,comprehensive gene annotation analysis,and evolution analysis.Furthermore,we compare the recovery rate of schistosomes,pathological changes,and liver transcriptomes between M.fortis and mice at different time points after infection.We observe that the time and type of immune response in M.fortis are different from those in mice.M.fortis activates immune and inflammatory responses on the 10th day post infection,such as leukocyte extravasation,antibody activation,Fc-gamma receptor-mediated phagocytosis,and the interferon signaling cascade,which play important roles in preventing the development of schistosomes.In contrast,an intense immune response occurrs in mice at the late stages of infection and could not eliminate schistosomes.Infected mice suffer severe pathological injury and continuous decreases in cell cycle,lipid metabolism,and other functions.Our findings offer new insights into the intrinsic resistance mechanism of M.fortis against schistosome infection.The genome sequence also provides the basis for future studies of other important traits in M.fortis.
基金supported in part by grants from the Chinese Academy of Sciences(KSCX2-YW-R-110,KSCX2-YW-R-229,XDA01010403 to J.L.,KSCX1-YW-02,KJCX2-YW-M15 to J.W.)the Ministry of Science and Technology(2007CB947101,2009CB941101 to J.L.,2006CB503900,2010CB912102 to J.W.)+1 种基金the National Natural Science Foundation of China(30871430 to J.L.,30821065 to J.W.)the Shanghai Municipal Commission for Science and Technology(07DZ22919,08DJ1400502,09PJ1410900 to J.L.,07dz05907 to J.W.).
文摘Dear Editor,Recent studies show that induced pluripotent stem cells(iPSCs)generated through ectopic expression of transcription factors retain an epigenetic memory of their original somatic cells(Kim et al.,2010;Polo et al.,2010)or aberrant silencing of a single imprinted gene cluster(Liu et al.,2010;Stadtfeld et al.,2010),which affects their developmental and differentiation potentials.
