With the help of the first principle calculation,the solid-state reaction experiment was conducted to investigate the alteration in the sintering and the microwave dielectric properties of Mg_(3)B_(2)O_(6)ceramic with...With the help of the first principle calculation,the solid-state reaction experiment was conducted to investigate the alteration in the sintering and the microwave dielectric properties of Mg_(3)B_(2)O_(6)ceramic with many Zn^(2+)substitutions.These properties were characterized using the scanning electron microscopy,network analyzer,X-ray diffraction,Raman spectroscopy,energy-dispersive spectroscopy,and thermomechanical and differential-thermal analyses.The coexistence of Mg_(3)B_(2)O_(6),Mg_(2)B_(2)O_(5)and ZnO ceramics could be observed with increasing Zn^(2+)addition,and the lattice distortion occurred in the Mg_(2)B_(2)O_(5)and Mg_(3)B_(2)O_(6)ceramics due to the substitution of Mg^(2+)with Zn^(2+).The electron density and the bond property of the MgO_(6)octahedron changed,and a quantitative method was used to discuss the variation in sintering,substitution and phase formation properties.The densification window was decreased to 1100℃,and the dielectric properties improved with the formation of a three-phase borate solid solution(dielectric constant=6.73,quality factor=112,000 GHz at 16 GHz(Q=7000),temperature coefficient of resonant frequency=-61.2 ppm℃^(-1),and relative density=97.0%).展开更多
[Objectives]The effects of"Glimmer"treatment on dry matter accumulation,yield and cadmium accumulation in different types of rice varieties were investigated,in order to provide a theoretical basis for the r...[Objectives]The effects of"Glimmer"treatment on dry matter accumulation,yield and cadmium accumulation in different types of rice varieties were investigated,in order to provide a theoretical basis for the regulation of low cadmium accumulation in rice grains.[Methods]Under the treatment of cadmium contaminated soil with a concentration of 0.58 mg/kg,the effects of"Glimmer"treatment on dry matter accumulation,yield and cadmium content of different rice varieties were studied by pot experiment.[Results]Spraying"Glimmer"can increase the biological yield of rice,and the different types of rice showed the order of conventional rice>two-line hybrid rice>three-line hybrid rice.Among the conventional rice,most significant increase was observed in Huanghuazhan,and in two-line hybrid rice,C Liangyou 651 increased the most.The"Glimmer"treatment had an obvious yield increasing effect on conventional rice,among which such two varieties as Xiangwanxian 13 and Huanghuazhan had the largest increases,followed by the two-line hybrid rice,and a little effect was observed on the three-line hybrid rice.The"Glimmer"treatment resulted in a significant reduction in cadmium content in different types of rice,which decreased by 13.33%-26.09%,and seven varieties showed a reduction over 21.74%.[Conclusions]The"Glimmer"treatment can significantly reduce the cadmium content in rice,and generally promote the dry matter accumulation and yield increase of rice,of which conventional rice performed better.展开更多
DNA methylation(DNAm)is one of the major epigenetic mechanisms in humans and is important in diverse cellular processes.The variation of DNAm in the human population is related to both genetic and environmental factor...DNA methylation(DNAm)is one of the major epigenetic mechanisms in humans and is important in diverse cellular processes.The variation of DNAm in the human population is related to both genetic and environmental factors.However,the DNAm profiles have not been investigated in the Chinese population of diverse ethnicities.Here,we performed double-strand bisulfite sequencing(DSBS)for 32 Chinese individuals representing four major ethnic groups including Han Chinese,Tibetan,Zhuang,and Mongolian.We identified a total of 604,649 SNPs and quantified DNAm at more than 14 million Cp Gs in the population.We found global DNAm-based epigenetic structure is different from the genetic structure of the population,and ethnic difference only partially explains the variation of DNAm.Surprisingly,non-ethnic-specific DNAm variations showed stronger correlation with the global genetic divergence than these ethnic-specific DNAm.Differentially methylated regions(DMRs)among these ethnic groups were found around genes in diverse biological processes.Especially,these DMR-genes between Tibetan and nonTibetans were enriched around high-altitude genes including EPAS1 and EGLN1,suggesting DNAm alteration plays an important role in high-altitude adaptation.Our results provide the first batch of epigenetic maps for Chinese populations and the first evidence of the association of epigenetic changes with Tibetans'high-altitude adaptation.展开更多
The most common and abundant DNA modification is 5-methylcytosine(5mC),which has been well-established as an epigenetic mark regulating gene expression in eukaryotes(Jones,2012).Another DNA modification N^6-methyl...The most common and abundant DNA modification is 5-methylcytosine(5mC),which has been well-established as an epigenetic mark regulating gene expression in eukaryotes(Jones,2012).Another DNA modification N^6-methyldeoxyadenosine(6mA),previously reported as a widespread DNA methylation in prokaryotes.展开更多
This paper discusses "geometric property(T)". This is a property of metric spaces introduced in earlier works of the authors for its applications to K-theory. Geometric property(T) is a strong form of "...This paper discusses "geometric property(T)". This is a property of metric spaces introduced in earlier works of the authors for its applications to K-theory. Geometric property(T) is a strong form of "expansion property", in particular, for a sequence(Xn)of bounded degree finite graphs, it is strictly stronger than(Xn) being an expander in the sense that the Cheeger constants h(Xn) are bounded below.In this paper, the authors show that geometric property(T) is a coarse invariant,i.e., it depends only on the large-scale geometry of a metric space X. The authors also discuss how geometric property(T) interacts with amenability, property(T) for groups,and coarse geometric notions of a-T-menability. In particular, it is shown that property(T) for a residually finite group is characterised by geometric property(T) for its finite quotients.展开更多
Embryonic stem (ES) cells are potent resources for cell therapy,and monoclonal antibodies (mAbs) against native cell surface markers of ES cells could be useful tools for therapeutic applications.Here,we report th...Embryonic stem (ES) cells are potent resources for cell therapy,and monoclonal antibodies (mAbs) against native cell surface markers of ES cells could be useful tools for therapeutic applications.Here,we report the development of a feasible approach,which could be used in mass production,for experimentally producing rabbit mAbs against native cell surface antigens on the cell surface.Two of the 14 mAbs,which were selected at random,could be bound to the cell surface antigens of mES cells.The immunocytochemistry (ICC) and Western blot results showed that mAb 39 recognises conformational epitopes.The target antigen of mAb 39 was then successfully purified using an improved immunoprecipitation approach in which mAb was bounded to intact mES cells before the cells were lysed.The LC-LTQ mass spectrum analysis showed that the target antigen of mAb 39 was Glut3.This result was further confirmed by Western blot using commercially available antibodies against Glut3.Further experiments showed that mAb 39 exhibited an antiproliferative effect on mES cells.We also found that Glut3 was differentially expressed among the mES cell population as detected by flow cytometry.展开更多
With the support of density functional theory(DFT)calculation,the amelioration of sintering and dielectric properties of the Mg_(3)B_(2)O_(6)(MBO)ceramic was realized through the substitution of magnesium with nickel....With the support of density functional theory(DFT)calculation,the amelioration of sintering and dielectric properties of the Mg_(3)B_(2)O_(6)(MBO)ceramic was realized through the substitution of magnesium with nickel.The TE-mode cylindrical cavity method was used to measure the dielectric properties at different frequencies.The thermo-mechanical analysis and simultaneous thermal analysis were used to characterize the chemical and mechanical properties.The phase composition was determined through the X-ray diffraction(XRD)and Raman spectrum.The microstructure was investigated using the scanning electron microscopy(SEM).Magnesium substitution with nickel(4 mol%)could ionize the B-0 bond of BO3,modify the vibration mode,improve the order degree,densify the microstmcture,decrease the intrinsic densification temperature,and ameliorate the dielectric properties of the MBO ceramics.The maximum values were achieved for the ceramics with 4 mol%nickel and sintered at 1175℃,that is,97.2%for relative density,72,600 GHz(10 GHz),75,600 GHz(11.4 GHz),and 92,200 GHz(15 GHz)for Q×f,7.1(10 GHz),7.01(11.4 GHz),and 6.91(15 GHz)for£r,and-56.3 ppm/℃ for if.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.61771104 and 62071106)Jiangxi Innovative Talent Program,and Sichuan Science and Technology Program(Grant No.2021JDTD0026)。
文摘With the help of the first principle calculation,the solid-state reaction experiment was conducted to investigate the alteration in the sintering and the microwave dielectric properties of Mg_(3)B_(2)O_(6)ceramic with many Zn^(2+)substitutions.These properties were characterized using the scanning electron microscopy,network analyzer,X-ray diffraction,Raman spectroscopy,energy-dispersive spectroscopy,and thermomechanical and differential-thermal analyses.The coexistence of Mg_(3)B_(2)O_(6),Mg_(2)B_(2)O_(5)and ZnO ceramics could be observed with increasing Zn^(2+)addition,and the lattice distortion occurred in the Mg_(2)B_(2)O_(5)and Mg_(3)B_(2)O_(6)ceramics due to the substitution of Mg^(2+)with Zn^(2+).The electron density and the bond property of the MgO_(6)octahedron changed,and a quantitative method was used to discuss the variation in sintering,substitution and phase formation properties.The densification window was decreased to 1100℃,and the dielectric properties improved with the formation of a three-phase borate solid solution(dielectric constant=6.73,quality factor=112,000 GHz at 16 GHz(Q=7000),temperature coefficient of resonant frequency=-61.2 ppm℃^(-1),and relative density=97.0%).
基金Supported by Hunan Provincial Financial FundHunan Hybrid Rice Research Center Project(38018136)
文摘[Objectives]The effects of"Glimmer"treatment on dry matter accumulation,yield and cadmium accumulation in different types of rice varieties were investigated,in order to provide a theoretical basis for the regulation of low cadmium accumulation in rice grains.[Methods]Under the treatment of cadmium contaminated soil with a concentration of 0.58 mg/kg,the effects of"Glimmer"treatment on dry matter accumulation,yield and cadmium content of different rice varieties were studied by pot experiment.[Results]Spraying"Glimmer"can increase the biological yield of rice,and the different types of rice showed the order of conventional rice>two-line hybrid rice>three-line hybrid rice.Among the conventional rice,most significant increase was observed in Huanghuazhan,and in two-line hybrid rice,C Liangyou 651 increased the most.The"Glimmer"treatment had an obvious yield increasing effect on conventional rice,among which such two varieties as Xiangwanxian 13 and Huanghuazhan had the largest increases,followed by the two-line hybrid rice,and a little effect was observed on the three-line hybrid rice.The"Glimmer"treatment resulted in a significant reduction in cadmium content in different types of rice,which decreased by 13.33%-26.09%,and seven varieties showed a reduction over 21.74%.[Conclusions]The"Glimmer"treatment can significantly reduce the cadmium content in rice,and generally promote the dry matter accumulation and yield increase of rice,of which conventional rice performed better.
基金the National Key Research and Development Program of China(2016YFC0900402)the Basic Science Center Program(32288101)+1 种基金the National Natural Science Foundation of China(32030020 and 31961130380)the Shanghai Municipal Science and Technology Major Project(2017SHZDZX01)。
文摘DNA methylation(DNAm)is one of the major epigenetic mechanisms in humans and is important in diverse cellular processes.The variation of DNAm in the human population is related to both genetic and environmental factors.However,the DNAm profiles have not been investigated in the Chinese population of diverse ethnicities.Here,we performed double-strand bisulfite sequencing(DSBS)for 32 Chinese individuals representing four major ethnic groups including Han Chinese,Tibetan,Zhuang,and Mongolian.We identified a total of 604,649 SNPs and quantified DNAm at more than 14 million Cp Gs in the population.We found global DNAm-based epigenetic structure is different from the genetic structure of the population,and ethnic difference only partially explains the variation of DNAm.Surprisingly,non-ethnic-specific DNAm variations showed stronger correlation with the global genetic divergence than these ethnic-specific DNAm.Differentially methylated regions(DMRs)among these ethnic groups were found around genes in diverse biological processes.Especially,these DMR-genes between Tibetan and nonTibetans were enriched around high-altitude genes including EPAS1 and EGLN1,suggesting DNAm alteration plays an important role in high-altitude adaptation.Our results provide the first batch of epigenetic maps for Chinese populations and the first evidence of the association of epigenetic changes with Tibetans'high-altitude adaptation.
基金supported by Recruitment program of Global Youth Expert of China (to X.G.)the Elite Youth Program of the Chinese Academy of Agricultural Science (to X.G.)the intramural research support from Biotechnology Research Institute, Chinese Academy of Agricultural Sciences
文摘The most common and abundant DNA modification is 5-methylcytosine(5mC),which has been well-established as an epigenetic mark regulating gene expression in eukaryotes(Jones,2012).Another DNA modification N^6-methyldeoxyadenosine(6mA),previously reported as a widespread DNA methylation in prokaryotes.
基金supported by the U.S.National Science Foundation(Nos.DMS1229939,DMS1342083,DMS1362772)
文摘This paper discusses "geometric property(T)". This is a property of metric spaces introduced in earlier works of the authors for its applications to K-theory. Geometric property(T) is a strong form of "expansion property", in particular, for a sequence(Xn)of bounded degree finite graphs, it is strictly stronger than(Xn) being an expander in the sense that the Cheeger constants h(Xn) are bounded below.In this paper, the authors show that geometric property(T) is a coarse invariant,i.e., it depends only on the large-scale geometry of a metric space X. The authors also discuss how geometric property(T) interacts with amenability, property(T) for groups,and coarse geometric notions of a-T-menability. In particular, it is shown that property(T) for a residually finite group is characterised by geometric property(T) for its finite quotients.
基金supported by the National Key Scientific Research Program of China,the Ministry of Science and Technology of the People’s Republic of China (No 2007947804)
文摘Embryonic stem (ES) cells are potent resources for cell therapy,and monoclonal antibodies (mAbs) against native cell surface markers of ES cells could be useful tools for therapeutic applications.Here,we report the development of a feasible approach,which could be used in mass production,for experimentally producing rabbit mAbs against native cell surface antigens on the cell surface.Two of the 14 mAbs,which were selected at random,could be bound to the cell surface antigens of mES cells.The immunocytochemistry (ICC) and Western blot results showed that mAb 39 recognises conformational epitopes.The target antigen of mAb 39 was then successfully purified using an improved immunoprecipitation approach in which mAb was bounded to intact mES cells before the cells were lysed.The LC-LTQ mass spectrum analysis showed that the target antigen of mAb 39 was Glut3.This result was further confirmed by Western blot using commercially available antibodies against Glut3.Further experiments showed that mAb 39 exhibited an antiproliferative effect on mES cells.We also found that Glut3 was differentially expressed among the mES cell population as detected by flow cytometry.
基金supported by the National Natural Science Foundation of China(Grant Nos.61771104 and 62071106)the Jiangxi Innovative Talent Program,and the Sichuan Science and Technology Program(Grant No.2021JDTD0026).
文摘With the support of density functional theory(DFT)calculation,the amelioration of sintering and dielectric properties of the Mg_(3)B_(2)O_(6)(MBO)ceramic was realized through the substitution of magnesium with nickel.The TE-mode cylindrical cavity method was used to measure the dielectric properties at different frequencies.The thermo-mechanical analysis and simultaneous thermal analysis were used to characterize the chemical and mechanical properties.The phase composition was determined through the X-ray diffraction(XRD)and Raman spectrum.The microstructure was investigated using the scanning electron microscopy(SEM).Magnesium substitution with nickel(4 mol%)could ionize the B-0 bond of BO3,modify the vibration mode,improve the order degree,densify the microstmcture,decrease the intrinsic densification temperature,and ameliorate the dielectric properties of the MBO ceramics.The maximum values were achieved for the ceramics with 4 mol%nickel and sintered at 1175℃,that is,97.2%for relative density,72,600 GHz(10 GHz),75,600 GHz(11.4 GHz),and 92,200 GHz(15 GHz)for Q×f,7.1(10 GHz),7.01(11.4 GHz),and 6.91(15 GHz)for£r,and-56.3 ppm/℃ for if.
基金supported by the National Natural Science Foundation of China(62375079,52072117,62375081,52221001,51972105,62090035,U19A2090,and 61905071)the National Key R&D Program of China(2022YFA1204300)+4 种基金the Key Program of Science and Technology Department of Hunan Province(2019XK2001 and 2020XK2001)the Key Research and Development Plan of Hunan Province(2023GK2012)the Open Project Program of Key Laboratory of Nanodevices and Applications,Suzhou Institute of Nano-Tech and Nano-Bionics,Chinese Academy of Sciences(22ZS01)the Hunan Provincial Natural Science Foundation of China(2021JJ30132)the China Scholarship Council.