Encoded by seven genes, angiopoietin-like （ANGPTL） family members structurally similar to the angiogenic regulating factor angiopoietin are known to possess biological activities in angiogenesis and metabolism. Here...Encoded by seven genes, angiopoietin-like （ANGPTL） family members structurally similar to the angiogenic regulating factor angiopoietin are known to possess biological activities in angiogenesis and metabolism. Here we reports for the first time the identification and expression analysis of all the seven members of bovine ANGPTL gene family, which were designated bANGPTL1 to bANGPTL7 in order. The seven bANGPTL genes consist of 4-9 exons, span 3800M-3000 bp and are located on different chromosomes. The deduced amino acid sequences of the members all possess an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, both characteristics of angiopoietins. Phylogenetic analysis showed that the 32 identified ANGPTL homologs from 9 species could be classified into two major groups. Real-time quantitative PCR （Q-PCR） analysis revealed that the bANGPTL family members have different expression patterns. This study will be helpful for investigation on the biological role of the bANGPTL family in this economically important species. Furthermore, it provides an insight into the molecular evolution of the emerging ANGPTL family展开更多
A new strategy was proposed to improve the utilization rate of corn straw by making non-ruminant feed products.The corn straw was ball milled,and the crushed straw was subjected to solid-state fermentation.After ball ...A new strategy was proposed to improve the utilization rate of corn straw by making non-ruminant feed products.The corn straw was ball milled,and the crushed straw was subjected to solid-state fermentation.After ball milling,the particle size of corn straw decreased signifcantly from 4.85±0.07 cm to 10–100μm,the content of neutral detergent fber and acid detergent fber decreased by 1.3%and 3.5%,respectively,and the total soluble sugar content increased by 16.2 mg/g.At the same time,the straw treated by ball milling as substrate could promote the rapid growth of Pediococcus acidilactici R30,the increase of organic acid production and further improvement of other nutrients during solid-state fermentation.In conclusion,the fermented straw products after ball milling could signifcantly improve the performance of pig feed in terms of digestibility and nutritional value,and better meet the feed needs of non-ruminant animals while saving resources.展开更多
基金supported by the Fundamental Research Funds for the Central Universities (Grant No. JUSRP10916)the Program of 'Qinglan Project' of Jiangsu Province
文摘Encoded by seven genes, angiopoietin-like （ANGPTL） family members structurally similar to the angiogenic regulating factor angiopoietin are known to possess biological activities in angiogenesis and metabolism. Here we reports for the first time the identification and expression analysis of all the seven members of bovine ANGPTL gene family, which were designated bANGPTL1 to bANGPTL7 in order. The seven bANGPTL genes consist of 4-9 exons, span 3800M-3000 bp and are located on different chromosomes. The deduced amino acid sequences of the members all possess an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, both characteristics of angiopoietins. Phylogenetic analysis showed that the 32 identified ANGPTL homologs from 9 species could be classified into two major groups. Real-time quantitative PCR （Q-PCR） analysis revealed that the bANGPTL family members have different expression patterns. This study will be helpful for investigation on the biological role of the bANGPTL family in this economically important species. Furthermore, it provides an insight into the molecular evolution of the emerging ANGPTL family
基金This work was supported by the National Natural Science Foundation of China(31701588)the Natural Science Foundation of Jiangsu Province(BK20170178)+2 种基金the Fundamental Research Funds for the Central Universities(JUSRP11965 and JUSRP21914)the Program of Introducing Talents of Discipline to Universities(111 project)(111-2-06)the Collaborative Innovation Center of Jiangsu Modern Industrial Fermentation and the Top-notch Academic Programs Project of Jiangsu Higher Education Institutions.
文摘A new strategy was proposed to improve the utilization rate of corn straw by making non-ruminant feed products.The corn straw was ball milled,and the crushed straw was subjected to solid-state fermentation.After ball milling,the particle size of corn straw decreased signifcantly from 4.85±0.07 cm to 10–100μm,the content of neutral detergent fber and acid detergent fber decreased by 1.3%and 3.5%,respectively,and the total soluble sugar content increased by 16.2 mg/g.At the same time,the straw treated by ball milling as substrate could promote the rapid growth of Pediococcus acidilactici R30,the increase of organic acid production and further improvement of other nutrients during solid-state fermentation.In conclusion,the fermented straw products after ball milling could signifcantly improve the performance of pig feed in terms of digestibility and nutritional value,and better meet the feed needs of non-ruminant animals while saving resources.
