To avoid the negative effects of antibiotics,using phage to prevent animal disease becomes a promising method in aquaculture.Here,a lytic phage provisionally named vB_Vca S_HC that can infect the pathogen(i.e.,Vibrio ...To avoid the negative effects of antibiotics,using phage to prevent animal disease becomes a promising method in aquaculture.Here,a lytic phage provisionally named vB_Vca S_HC that can infect the pathogen(i.e.,Vibrio campbellii 18)of prawn was isolated.The phage has an isometric head and a non-contractile tail.During phage infection,the induced host mortality in 5.5 h reached ca.96%,with a latent period of 1.5 h and a burst size of 172 PFU/cell.It has an 81,566 bp circular ds DNA genome containing 121 open reading frames(ORFs),and ca.71%of the ORFs are functionally unknown.Comparative genomic and phylogenetic analysis revealed that it is a novel phage belonging to Delepquintavirus,Siphoviridae,Caudovirales.In the phage genome,besides the ordinary genes related to structure assembly and DNA metabolism,there are 10 auxiliary metabolic genes.For the first time,the pyruvate phosphate dikinase(PPDK)gene was found in phages whose product is a key rate-limiting enzyme involving Embden-Meyerhof-Parnas(EMP)reaction.Interestingly,although the phage has a strong bactericidal activity and contains a potential lysogeny related gene,i.e.,the recombinase(Rec A)gene,we did not find the phage turned into a lysogenic state.Meanwhile,the phage genome does not contain any bacterial virulence gene or antimicrobial resistance gene.This study represents the first comprehensive characterization of a lytic V.campbellii phage and indicates that it is a promising candidate for the treatment of V.campbellii infections.展开更多
基金supported by the open task of Qingdao National Laboratory for Marine Science and Technology(QNLM2016ORP0311)the NSFC projects (41876174, U1906216)+4 种基金the Senior User Project of RV KEXUE (KEXUE2019GZ03) supported by Center for Ocean Mega-Science,Chinese Academy of Sciencesthe DICP&QIBEBT (DICP&QIBEBT UN201803)the QIBEBT (QIBEBT ZZBS 201805)Dalian National Laboratory For Clean Energy (DNL),CASCentral Public-interest Scientific Institution Basal Research Fund,CAFS (No. 2017HY-ZD1002)。
文摘To avoid the negative effects of antibiotics,using phage to prevent animal disease becomes a promising method in aquaculture.Here,a lytic phage provisionally named vB_Vca S_HC that can infect the pathogen(i.e.,Vibrio campbellii 18)of prawn was isolated.The phage has an isometric head and a non-contractile tail.During phage infection,the induced host mortality in 5.5 h reached ca.96%,with a latent period of 1.5 h and a burst size of 172 PFU/cell.It has an 81,566 bp circular ds DNA genome containing 121 open reading frames(ORFs),and ca.71%of the ORFs are functionally unknown.Comparative genomic and phylogenetic analysis revealed that it is a novel phage belonging to Delepquintavirus,Siphoviridae,Caudovirales.In the phage genome,besides the ordinary genes related to structure assembly and DNA metabolism,there are 10 auxiliary metabolic genes.For the first time,the pyruvate phosphate dikinase(PPDK)gene was found in phages whose product is a key rate-limiting enzyme involving Embden-Meyerhof-Parnas(EMP)reaction.Interestingly,although the phage has a strong bactericidal activity and contains a potential lysogeny related gene,i.e.,the recombinase(Rec A)gene,we did not find the phage turned into a lysogenic state.Meanwhile,the phage genome does not contain any bacterial virulence gene or antimicrobial resistance gene.This study represents the first comprehensive characterization of a lytic V.campbellii phage and indicates that it is a promising candidate for the treatment of V.campbellii infections.
