Isolation and Identification of a High-pathogenicity Strepto- coccus suis Serotype 7 Strain

In order to diagnose the diseased pigs in a certain large pig farm in Binzhou City, Shandong Province, the dead piglets with joint swelling were subjected to necroscopy, and the pathogenic bacterium was isolated and identified. One Gram-positive Streptococcus was isolated. The strain was subjected to characteristic culture, microscopic examination and molecular biological identification, and resistance detection, animal regression experiment and mouse pathogenicity test were carried out. The results showed that the isolate was identified to be Streptococcus suis serotype 7, which was resistant to multiple drugs; and the pathogenicity test showed that the strain had high pathogenicity to pigs, resulting in neurosis on partial pigs, and the strain had no pathogenicity to Kunming and BALB/c mice but certain pathogenicity to CD1 mice.

Hybrid 3D printed three-axis force sensor aided by machine learning decoupling

Identification of magnitude and orientation for spatially applied loading is highly desired in the fields of not only the machinery components but also human-machine interaction.Despite the fact that the 3-axis force sensor with different structures has been proposed to measure the spatial force,there are still some common limitations including the multi-step manufacturing-assembly processes and complicated testing of decoupling calibration.Here,we propose a rapid fabrication strategy with low-cost to achieve high-precision 3-axis force sensors.The sensor is designed to compose of structural Maltese cross base and sensing units.It is directly fabricated within one step by a hybrid 3D printing technology combining deposition modeling(FDM)with direct-ink-writing(DIW).In particular,a machine learning(ML)model is used to convert the strain signal to the force components.Instead of a mount of calibration tests,this ML model is trained by sufficient simulation data based on programmed batch finite element modeling.This sensor is capable of continuously identifying a spatial force with varying magnitude and orientation,which successfully quantify the applied force of traditional Chinese medicine physiotherapy including Gua Sha and massage.This work provides insight for design and rapid fabrication of multi-axis force sensors,as well as potential applications.

Naphthalimide-modified near-infrared cyanine dye with a large stokes shift and its application in bioimaging

Developing the novel fluorescent dyes with a larger Stokes shift is still a challenge in the research of fluorescence probes. In this work, a naphthalimide-modified near-infrared cyanine dye with an emission at 785 nm has been synthesized for lysosome-targeting imaging. This fluorescent dye showed a large Stokes shift（up to 165 nm） and favorable lysosome-targeting property, which facilitated it to be a potential candidate for studying of lysosomal functions. The result also indicated that the probe is a promising contrast agent for in vivo imaging in mouse models.