OsFK1 encodes C-14 sterol reductase,which is involved in sterol biosynthesis and affects premature aging of leaves in rice

The enzyme C-14 sterol reductase is involved in biosynthesis of brassinosteroids(BR)and sterols,as well as plant development.OsFK1,a member of the sterol biosynthesis pathway located in the endoplasmic reticulum(ER),encodes C-14 sterol reductase.However,there is little research on the function of C-14 sterol reductase in rice.Compared with the wild type,an osfk1 mutant showed dwarf phenotype and premature aging in the second leaf during the trefoil stage,and abnormal development of leaf veins during the tillering stage.The osfk1 mutant showed signs of aberrant PCD,as evidenced by TUNEL staining.This suggested that high ROS buildup caused DNA damage and ROS-mediated cell death in the mutant.The osfk1 mutant also showed decreased chlorophyll content and aberrant chloroplast structure.Sequencing of the osfk1 mutant allele revealed a non-synonymous G to A mutation in the final intron,leading to early termination.Here,we identified the OsFK1 allele,cloned it by Mutmap sequencing,and verified it by complementation.HPLC-MS/MS assays demonstrated that the osfk1 mutation caused lower phytosterol levels.These findings showed that the OsFK1 allele encoding C-14 sterol reductase is involved in phytosterol biosynthesis and mediates normal development of rice plants.

Study on the dynamic contact relationship between layers under temperature gradients in CRTSⅢ ballastless track

In areas with large temperature differences,the uneven distribution of temperatures in the CRTS III ballastless track slab due to daytime sunlight can cause warpage deformation,leading to periodic rail irregularities that increase the wheel-rail impact of high-speed vehicles and accelerate track structure damage.Therefore,it is necessary to study the dynamic contact relationship between the composite slab and the base plate during vehicle running.The results of the study show that:1)Under the influence of temperature gradients,the composite slab tends to deform elliptically.With a positive temperature gradient,the middle part of the track slab bulges upward,causing the slab to be supported by its four corners.Conversely,with a negative temperature gradient,the four corners of the track slab bulge upward,resulting in the slab being supported by its center.2)Temperature gradients can lead to separation between the composite slab and the base plate,reducing the contact area between layers.During vehicle running,the contact area between layers gradually increases,but the separation cannot be completely closed.3)The temperature gradient significantly affects the vertical displacement of the track.The vertical displacement in the middle of the slab increases with a positive temperature gradient.In contrast,the vertical displacement at the ends of the slab increases with a negative temperature gradient.4)The stress of self-compacting concrete at the side position significantly increases under a positive temperature gradient,with the vertical stress increasing by 2.7 times when the temperature gradient increases from 0 to 90℃·m^(-1).

Use of Plastic Correction Formula to Improve Accuracy of Welding Residual Stress Test with Blind-Hole Method

The blind-hole method is the most widely used approach to experimentally determine the distribution of residual stress. This paper aims to improve test accuracy of welding residual stress and conducts an experimental study on the strain release factors involved when using the blind-hole method for Q235 and Q345, two steels commonly used in building structures. The ranges of strain release factors A and B in the elastic stage, the effects of strain release factors on residual stress calculated values, and the plastic corrected strain release factors are analyzed considering of the effect of plastic deformation around the blind hole on measurement accuracy. Finally, a simplified calculation formula to determine strain release factors is proposed for use with the blind-hole method. Results show that in the elastic stage, strain release factor A for Q235 and Q345 ranges from-0.399 to-0.525 and strain release factor B from-0.791 to-0.960. Changing the strain release factors A and B shows that calculated residual tensile stress varies in relation to a decrease in both factor values. However, there is a increase in calculated residual compressive stress with a decrease in the strain release factor A value, but there is an decrease with a decrease in strain release factor B value. Calculated residual stress applied to elastic strain release factors is compared with that applied to amended plastic strain release factors for Q235 steel. The maximum deviation between calculated residual stress and test stress is reduced from 21.1 to 1.0%,and for Q345 steel from 26.5 to 1.2%. It is thus evident that the plastic correction formula proposed in this paper can be used in calculations when conducting a residual stress test.

Study on the influencing factors of peasants' subjective well-being

On the basis of data collected from Liupanshan poverty-ridden areas,the paper selects 24 variables under 4 groups to figure out the influencing factors of subjective well-being(SWB) of peasants in ecological migration with the method of Ordered Probit Regression.As is shown in the results,variables under peasants' personal endowment group and resource of the development group have little impact on peasants' SWB.The variables with observable impact are concentrated in the living condition group and the public atmosphere group.

Investigation of monitoring system for high-speed railway subgrade frost heave

This paper presents methods for monitoring frost heave, device requirements, testing principals, and data analysis re- quirements, such as manual leveling observation, automatic monitoring （frost heave, frost depth, and moisture）, track dynamic detection, and track status detection. We focused on the requirements of subgrade frost heave monitoring for high speed railways, and the relationship of different monitoring methods during different phases of the railway. The com- prehensive monitoring system of high speed railway subgrade frost heave provided the technical support for dynamic design during construction and safe operation of the rail system.

基于自适应采样的高超声速飞行器气动热全局快速预示

高超声速飞行器热防护系统设计中高精度气动热分析模型使得设计计算成本不断增加,基于数据驱动的气动热环境预示方法受到广泛关注。针对有限高精度模型计算成本下提升全局预示精度的问题,提出一种基于模糊聚类的批量自适应采样方法。根据预示误差分布特征通过聚类采用超球分割构建采样影响域,兼顾误差较大的重点采样域与全局探索;通过当地误差评分系数加权构建采样拒绝域,减小新增样本冗余;结合maxmin准则在综合确定的重点采样空间中新增样本,提升采样质量,进而实现预示模型全局精度快速提升。数值测试算例表明,所提方法与One-Shot、APSFC、CV-Voronoi方法相比能有效降低所需采样规模,加速提升预示精度。通过类HTV-2飞行器气动热快速预示实例,验证了方法的有效性与工程实用性。

A statistical study on the correlation between sporadic Ca+layer and Es in Beijing(40.5°N,116°E)

From April 2020 to June 2022,a Ca+lidar with dual-wavelength and narrow-band emitting lasers utilizing all-solid-state technology was employed to conduct observations in Beijing(40.41°N,116.01°E)for a total duration of 1797.79 hours over 280 nights.A total of 286 sporadic Ca+layers were observed,with heights ranging from 88 to 120 km and densities varying from 5.33 to 2200 cm^(-3).We simultaneously observed the ionosonde,located 28 km from the lidar,and found a correlation of 61.89%between the sporadic Ca+layer and the sporadic E layer.When the sporadic Ca+layer has a downward phase trend,there is a correlation of 76.84%.By excluding the influence of ionosonde sensitivity,we focus only on calcium ions that have a density exceeding 140 cm^(-3),which exhibit a correlation of82.25%.Above 100 km,the correlation significantly increases,reaching approximately 90%.Furthermore,the correlation between the sporadic Ca+layer and the sporadic E layer is particularly noticeable during the summer season,reaching around 90%.This phenomenon can be attributed to the variations in metal ions that occur during different seasons.

Recent advances in recyclable thermosets and thermoset composites based on covalent adaptable networks

Recyclable thermosets and thermoset composites with covalent adaptable networks(CANs,or dynamic covalent networks) have attracted considerable attention in recent years due to the combined merits of excellent mechanical and thermal properties,and chemical stabilities of traditional thermosets and recyclable,remoldable,and reprocessable attributes of thermoplastics.In this paper,we present an overview of the current strategies for synthesizing recyclable thermosets based on CANs,which involve recyclability,reprocessability,and possible rehealability.The recent literature examples are categorized based on the underlying controlled-cleavable linkages such as transesterification,DA/retro-DA chemistry,imine bonds,disulfide metathesis,dynamic B-O bonds,hemiaminals/hexahydrotriazines,and acetal linkages.Various degradation and malleability methods and resulting mechanical properties of the recycled thermosets and thermoset composites are presented.The emerging applications of recyclable thermosets and thermoset composites,with emphasis on their usage in adhesives,biomedical materials,wearable devices,coatings,and 3D printing materials,are also illustrated.Finally,a perspective on the challenges and future perspectives is briefly summarized.

Joint observations of sporadic sodium and sporadic E layers at middle and low latitudes in China

Sporadic sodium layers(SSLs)were studied with sodium(Na)lidar at Beijing(40.5°N,116.0°E)and Haikou(19.5°N,109.1°E)in China.The altitude distribution,strength factors,and occurrence time of SSL and sporadic E(Es)layer events were statistically analyzed at both observing sites.SSL occurrence had a maximum near22:00 local time,and Eslayer occurrence had a maximum before midnight.The altitude distributions of SSL and Es layer events were better correlated at Haikou than at Beijing.All the average values of height and strength factor for SSL and Eslayer events,as well as the average of the maximum frequency that can be reflected by the Eslayer(foEs),at Beijing were higher than those at Haikou.A better correlation between SSL and Eslayer events was also found at Haikou,and the formation of SSLs was considered to likely depend on the seasonal variability of chemical and dynamical processes.