A Double Adaptive Random Spare Reinforced Sine Cosine Algorithm

Many complex optimization problems in the real world can easily fall into local optimality and fail to find the optimal solution,so more new techniques and methods are needed to solve such challenges.Metaheuristic algorithms have received a lot of attention in recent years because of their efficient performance and simple structure.Sine Cosine Algorithm(SCA)is a recent Metaheuristic algorithm that is based on two trigonometric functions Sine&Cosine.However,like all other metaheuristic algorithms,SCA has a slow convergence and may fail in sub-optimal regions.In this study,an enhanced version of SCA named RDSCA is suggested that depends on two techniques:random spare/replacement and double adaptive weight.The first technique is employed in SCA to speed the convergence whereas the second method is used to enhance exploratory searching capabilities.To evaluate RDSCA,30 functions from CEC 2017 and 4 real-world engineering problems are used.Moreover,a nonparametric test called Wilcoxon signed-rank is carried out at 5%level to evaluate the significance of the obtained results between RDSCA and the other 5 variants of SCA.The results show that RDSCA has competitive results with other metaheuristics algorithms.

Oncolytic Activity of Wild-type Newcastle Disease Virus HK84 Against Hepatocellular Carcinoma Associated with Activation of Type I Interferon Signaling

Background and Aims:Hepatocellular carcinoma(HCC)is listed as one of the most common causes of cancer-related death.Oncolytic therapy has become a promising treatment because of novel immunotherapies and gene editing technology,but biosafety concerns remain the biggest limitation for clinical application.We studied the the antitumor activity and biosafety of the wild-type Newcastle disease virus HK84 strain(NDV/HK84)and 10 other NDV strains.Meth-ods:Cell proliferation and apoptosis were determined by cell counting Kit-8 and fluorescein isothiocyanate Annexin V apoptosis assays.Colony formation,wound healing,and a xenograft mouse model were used to evaluate in vivo and in vitro oncolytic effectiveness.The safety of NDV/HK84 was tested in nude mice by an in vivo luciferase imaging system.The replication kinetics of NDV/HK84 in normal tis-sues and tumors were evaluated by infectious-dose assays in eggs.RNA sequencing analysis was performed to explore NDV/HK84 activity and was validated by quantitative real-time PCR.Results:The cell counting Kit-8 assays of vi-ability found that the oncolytic activity of the NDV strains differed with the multiplicity of infection(MOI).At an MOI of 20,the oncolytic activity of all NDV strains except the DK/JX/21358/08 strain was>80%.The oncolytic activities of the NDV/HK84 and DK/JX/8224/04 strains were>80%at both MOI=20 and MOI=2.Only NDV/HK84 had>80%oncolytic activities at both MOI=20 and MOI=2.We chose NDV/HK84 as the candidate virus to test the oncolytic effect of NDV in HCC in the in vitro and in vivo experiments.NDV/HK84 killed human SK-HEP-1 HCC cells without affecting healthy cells.Conclusions:Intratumor infection with NDV/HK84 strains compared with vehicle controls or positive controls indicated that NDV/HK84 strain specifically inhib-ited HCC without affecting healthy mice.High-throughput RNA sequencing showed that the oncolytic activity of NDV/HK84 was dependent on the activation of type I interferon signaling.