The effect of pentoxifylline on IL-6 and PDGF expression in human keloid fibroblasts

Objective:The aim of this study was to investigate the effect of different dose of pentoxifylline(PTX)on intedeukin-6(IL-6)and platelet-derived growth factor(PDGF)expression in human normal skin fibroblast(NF)and keloid fibroblast(KF),to explore the pathogenesis of PTX on prevention and treatment of KF.Methods:Human NF and KF were passaged till the 5 th to 7 th generations by tissue block adhering wall method,and then were cultured in vitro with different dose of PTX.Levels of IL-6 and PDGF were detected by ELISA at various periods.Results:(1)Compared with control group,the expressions of IL-6 in NF were significantly inhibited with 0.5 mg/ml PTX for 72 h,1.0 mg/ml and 2.0 mg/ml PTX for 24 h,48 h and 72 h separately(p<.01);The lower expressions of IL-6 were also showed in KF with 0.25 mg/ml PTX for 48 h and 72 h,0.5 mg/ml,1.0 mg/ml and 2.0 mg/ml PTX for 24 h,48 h and 72 h separately(p<.01).(2)After cultured with 0.25 mg/ml PTX for 72 h,0.5 mg/ml,1.0 mg/ml and 2.0 mg/ml PTX for 24 h,48 h and 72 h separately,the expressions of PDGF in NF were decreased significantly compared to the controls(p<.01);The reduced PDGF expressions were also found in KF after cultured with 0.25 mg/ml,0.5 mg/ml,1.0 mg/ml and 2.0 mg/ml PTX for 24 h,48 h and 72 h separately(p<.05).Repeated measures analysis of variance indicated the difference source of cells and doses with statistical significance.Conclusions:The expressions of IL-6 and PDGF in KF were higher than those in NF.PTX plays a notable inhibitory role in the expression of IL-6 and PDGF,which could be used as a promising medicine for Keloid treatment.