Blue LED promotes the chemosensitivity of human hepatoma to Sorafenib by inducing DNA damage

Background:Phototherapies based on sunlight,infrared,ultraviolet,visible,and laser-based treatments present advantages like high curative effects,small invasion,and negligible adverse reactions in cancer treatment.We aimed to explore the potential therapeutic effects of blue light emitting diode(LED)in human hepatoma cells and decipher the underlying cellular and molecular mechanisms.Methods:Wound healing and transwell assays were employed to probe the inhibition of the invasion and migration of hepatocellular carcinoma cells in the presence of blue LED.The sphere-forming test was used to evaluate the effect of LED blue light irradiation on cancer stem cell properties.Immunofluorescence and western blotting were used to detect the changes inγ-H2AX.The Cell Counting Kit-8 assay,5-ethynyl-2′-deoxyuridine staining,and colony formation assay were used to detect the combined effect of blue LED and sorafenib on cell proliferation inhibition.Results:We demonstrated that the irradiation of blue LED light in hepatoma cells could lead to cell proliferation reduction along with the increase of cell apoptosis.Simultaneously,blue LED irradiation also markedly suppressed the migration and invasion ability of human hepatoma cells.Sphere formation analysis further revealed the decreased cancer stemness of hepatoma cells upon blue LED irradiation.Mechanistically,blue LED irradiation significantly promoted the expression of the phosphorylation of the core histone protein H2AX(γ-H2AX),a sensitive molecular marker of DNA damage.In addition,we found that the combined treatment of blue LED irradiation and sorafenib increased cancer cell sensitivity to sorafenib.Conclusion:Collectively,we demonstrated that blue LED irradiation exhibited anti-tumor effects on liver cancer cells by inducing DNA damage and could enhance chemosensitivity of cancer cells,which represents a potential approach for human hepatoma treatment.

OPTIMAL DIVIDEND-PENALTY STRATEGIES FOR INSURANCE RISK MODELS WITH SURPLUS-DEPENDENT PREMIUMS

This paper concerns an optimal dividend-penalty problem for the risk models with surplus-dependent premiums.The objective is to maximize the difference of the expected cumulative discounted dividend payments received until the moment of ruin and a discounted penalty payment taken at the moment of ruin.Since the value function may be not smooth enough to be the classical solution of the HJB equation,the viscosity solution is involved.The optimal value function can be characterized as the smallest viscosity supersolution of the HJB equation and the optimal dividend-penalty strategy has a band structure.Finally,some numerical examples with gamma distribution for the claims are analyzed.

利用发酵饲料养猪效果好

发酵饲料作为新型环保型饲料,具有适口性好,活菌含量高,营养更全面,绿色无残留等特点。利用其养猪能够调节猪肠道的微生态平衡,提高饲料利用率,降低饲养成本,增强免疫功能,减少发病死亡,提高生产性能,改善酮体品质,减少环境污染,是饲料“禁抗”后的最佳替代物,代表着绿色健康、生态环保、节粮型畜牧业的发展方向,有很高的应用价值和广阔的市场前景。

Research on Model of Virtual Organization and Its Practice

This paper discusses an organizational model to be used for both conventional and virtual organizations. The model deals with variable relationships within an organization and provides a framework for overall organizational design that may include relationship among different design variables and external relationship with environment. Based on the researches of virtual organization, this paper also illustrates the new model of organization in the real world such as Beijing 2008 Olympic games and Dongfeng Automobile group.

Exponential change of measure for general piecewise deterministic Markov processes

We consider a general piecewise deterministic Markov process(PDMP) X = {X_t}_(t≥0) with a measure-valued generator A, for which the conditional distribution function of the inter-occurrence time is not necessarily absolutely continuous. A general form of the exponential martingales that are associated with X is given by■By considering this exponential martingale to be a likelihood-ratio process, we define a new probability measure and show that the process X is still a general PDMP under the new probability measure. We additionally find the new measure-valued generator and its domain. To illustrate our results, we investigate the continuous-time compound binomial model.

Ruin probability in Sparre Andersen risk model with claim inter-arrival times distributed as Erlang

This article deals with the ruin probability in a Sparre Andersen risk process with the inter-claim times being Erlang distributed in the framework of piecewise deterministic Markov process （PDMP）. We construct an exponential martingale by virtue of the extended generator of the PDMP to change the measure. Some results are derived for the ruin probabilities, such as the general expressions for ruin probability, Lundberg bounds, CramerLundberg approximations, and finite-horizon ruin probability.