PET/CT联合CT三维重建在鉴别早期肺腺癌不同病理亚型中的价值

背景与目的早期肺腺癌中病理亚型以贴壁为主型的浸润性腺癌(lepidic predominant invasive adenocarcinoma,LPA)与原位腺癌(adenocarcinomainsitu,AIS)、微浸润性腺癌(microinvasiveadenocarcinoma,MIA)的良好预后相似,临床上也迫切需要能够区分LPA与非LPA型浸润性腺癌(non-lepidicpredominantinvasive adenocarcinoma,non-LPA)的手段,本研究拟通过正电子发射型计算机断层显像(positronemissioncomputed tomography, PET)/计算机断层扫描(computed tomography, CT)的最大标准化摄取值(maximal standard uptake value,SUVmax)和CT三维重建后参数探讨术前影像学表现为部分实性结节(part-solidnodules,PSNs)的早期肺腺癌不同病理亚型间的关系。方法回顾性分析2016年1月-2019年1月于江苏省苏北人民医院胸外科行解剖性肺切除术且影像学表现为PSNs的早期肺腺癌患者资料,所有患者胸部增强CT和PET/CT资料完整可获取,利用Mimics软件行三维重建,获取肿瘤体积、肿瘤三维平均CT值(3-dimensional mean-CT value, 3Dm-CT)、SUVmax等数据,采用SPSS 25.0进行统计分析,Graph Pad Prism 8.3.0绘制受试者工作曲线(receiver operating curve, ROC),P<0.05为差异有统计学意义。结果最终共计67例患者纳入本研究,按病理亚型不同将所有患者分为两组,AIS、MIA及浸润性腺癌(invasive adenocarcinoma, IAC)中的LPA归为低危组28例(41.8%),其余non-LPA如腺泡型(acinar pattern-predominantadeno-carcinoma,APA)、乳头型(papillarypattern-predominantadenocarcinoma,PPA)、微乳头型(micropapillary pattern-predominant adeno-carcinoma, MPA)归为高危组39例(58.2%),两组间SUVmax(t=3.153,P=0.002)、肿瘤体积(t=3.331, P=0.001)、实性/磨玻璃成分体积(t=2.74, P=0.006)/(t=3.127, P=0.002)、实性/磨玻璃成分3Dm-CT(t=3.655, P<0.001)/(t=7.082, P<0.001)均具有显著统计学意义。ROC曲线提示:SUVmax[曲线下面积(area under curve, AUC)=0.727]、肿瘤体积(AUC=0.740)、磨玻璃成分体积(AUC=0.725)、实性成分3Dm-CT(AUC=0.763)、磨玻璃成分3Dm-CT(AUC=0.756)预测效能最佳。将上述AUC>0.7的协变量纳入多因素ROC曲线分析,获得联合预测因子(AUC=0.835)具有中等以上预测价值。结论 PET/CT中SUVmax和CT三维重建参数与影像学表现为PSNs的早期肺腺癌的不同病理亚型具有显著相关性,联合SUVmax、肿瘤体积、磨玻璃成分体积和实性/磨玻璃成分3Dm-CT对鉴别表现为PSNs的早期肺腺癌的病理亚型具有一定价值。

Effects of two different glycoprotein platelet Ⅱb/Ⅲa inhibitors and the clinical endpoints in patients with intracranial Pipeline flow diverter implant

Objective:To compare the antiplatelet effect and major adverse cerebrovascular events of Pipeline for intracranial aneurysms using glycoproteinⅡb/Ⅲa antagonists(GPI)eptifibatide and tirofiban.Methods:Retrospective analysis of relevant data of patients using GPIs combined with oral antiplatelet therapy in Nanfang Hospital of Southern Medical University from December 2017 to December 2019.The study was approved by the ethics Committee of Nanfang Hospital of Southern Medical University.According to the random use of GPIs drugs,they were assigned to the eptifibatide group and tirofiban group.Basic data,platelet inhibition rates at baseline,24 h and 72 h after administration,short-term major adverse cerebrovascular events,and bleeding complications were compared between the two groups.Results:A total of 47 patients were included in this study,including 24 patients in eptifibatide group and 23 patients in tirofiban group.There was no significant difference in average age(53.75 vs.53.91 years)and body mass index(BMI)(24.39 vs.22.73 kg/m2)between eptifibatide group and tirofiban group.There was no significant difference in coagulation factor function(R),fibrinogen function(K),fibrinolysis function(EPL),comprehensive coagulation index(Cl),arachidonic acid pathway inhibition rate(AA%)and adenosine diphosphate inhibition rate(ADP%).However,the baseline level of residual platelet function MA(ADP)in eptifibatide group was significantly higher than that in tirofiban group(50.79 vs.35.29 mm,P=0.0026).There was a statistical difference in the platelet aggregation function MA(65.38 vs.62.54 mm,p=0.0442),the rate of spontaneous hemorrhagic stroke(4.3%vs.0%)and the rate of asymptomatic minor bleeding(26.08%vs.4.1%)in the two groups(P<0.05).Conclusion:Both eptifibatide and tirofiban can effectively inhibit platelets,but the effect of etifeptide is better than that of tirofiban in preventing intracranial microhemorrhage and asymptomatic cerebral infarction.

Electrokinetic Phenomena of Modified Polytetrafluoroethylene Membranes in the Oily Sewage from Oil Field

Experimental investigations of electrokinetic phenomena of modified polytetrafluoroethylene membranes in the oily sewage from oil field were performed by using the streaming potential method. The zeta potentials of the membranes in the oily waste water are estimated on the basis of Helmholtz—Smoluchowski equation. The experiment and calculation results show that the membranes are charged negatively, whose zeta poten-tials maintain at around -20mV. And the aperture of membranes, the temperature and the filtration flux have little influence on the streaming potentials and the zeta potentials of the membranes.Also the suspended particulates in the oily sewage are charged negatively. The membranes have strong ability to withhold the suspended substance and powerful antipollution competence because of the role of the charges on the mem-branes.

Molecular Analysis of Pathogenicity Differences of Avian Paramyxovirus 1 Genotypes VI and VII in Chickens

Background:Genotypes VI and VII of(APMV-1)have different host range and pathogenicity in pigeons and chickens.However,the molecular determinants of these differences are still unclear.Methods:Here,we aligned the DNA sequences of 56 genotype VI and 33 genotype VII APMV-1 strains.Sequence alignment results revealed that there are 17 amino acids sites differed between APMV-1 strains of these two genotypes.We then constructed a plasmid based on the full-length genome of rSG10 APMV-1 strain,which belongs to genotype VII but was mutated with these 17 VI-genotype-specific amino acids,and rescued as rSG10-17 strain.The restriction digestion and ligation and overlapping PCR methods were used in the construction of plasmids with amino acids mutation.This virus was evaluated for its virulence and growth characteristics.Results and conclusion:The results indicated that the virulence and the growth characteristics have no obvious difference between the rSG10-17 virus and its parental strain rSG10.The simultaneous mutation of 17 genotype-specific amino acids did not affect the virulence of APMV-1 in chickens.Further analysis of these amino acids is required by taking into consideration of the functions of encoded proteins.

The Influence of Newcastle Disease Virus Major Proteins on Virulence

The Newcastle disease virus(NDV)negative-strand RNA genome contains six genes.These genes encode nucleoprotein(NP),phosphoprotein(P),matrix protein(M),fusion protein(F),hemagglutinin-neuraminidase(HN),and RNA-dependent RNA polymerase(L)proteins.The six proteins affect the virulence of NDV in different ways,but available information on the six proteins is disparate and scattered across many databases and sources.A comprehensive overview of the proteins determining NDV virulence is lacking.This review summarizes the virulence of NDV as a complex trait determined by these six different proteins.

Optimization of the key position parameters for tractor steering wheel based on a driver’s arm muscle load analysis

Steering wheel is the most frequently used manual device in tractors,whose position directly affects the handling comfort of the driver and fatigue degree of the arm muscles.In this study,the biomechanical modelling software AnyBody was used for an inverse kinetics analysis of the rotation process of tractor steering wheel,calculate the muscle activation degree of the driver’s arm and compare it with the calculated results of surface EMG tests to verify the reliability of the biomechanical model.Based on the biomechanical model,the effects of three position parameters(steering wheel inclination,front-back distance,and upper-lower height)on the activation degree of the driver’s arm muscles were evaluated.The results demonstrated that steering wheel inclination has the most significant effect on the degree of muscle activation,followed by the upper-lower height and then front-back distance.Considering the interaction among factors,a regression orthogonal test was designed,and the test results revealed that the minimum muscle activation(1.2887)can be obtained with the steering wheel inclination of 31°,front-back distance of 431 mm and upper-lower height of 375 mm.The findings can provide a reference for optimizing the structure and position parameters of tractor steering wheels.

Design and experiment of centralized pneumatic deep precision fertilization device for rice transplanter

To improve the precision of deep fertilization of paddy fields,a six-row centralized pneumatic deep precision fertilization device for a rice transplanter was designed.This device included a spiral fertilizer distribution system,centralized pneumatic fertilizer delivery system,an opener system,and a fertilization control system.The centralized airflow distribution method was used in the fertilizer delivery system to ensure that the airflow in each fertilizer pipe was evenly distributed.The rotational speeds of the power take-off(PTO)and fertilizer shaft were measured synchronously using photoelectric sensors and matched proportionately in real-time using PID closed-loop control algorithms to achieve precise fertilization rates at each working speed of the rice transplanter.There were two key considerations in the design of the control system to ensure precise fertilization.Firstly,a photoelectric sensor was used to measure the speed of the PTO;the high rotational speed of the PTO could provide a high signal frequency and improve the precision of the measurement of the transplanter’s working speed.Secondly,the fertilizer shaft speed measurement subprogram was set to sleep for a short period to reduce the vibration caused by the engine.During the tests of pneumatic fertilizer delivery system,single-factor tests on airflow distribution methods were conducted.The results showed that the coefficient of variation of the airflow speed for the centralized airflow distribution method was 1.67%,which was the least among the coefficients of the three distribution methods.In the bench tests,the rotational speeds of the fertilizer shaft were set at 10 r/min,20 r/min,30 r/min,and 40 r/min.The maximum coefficient of variation of the fertilization consistency in different rows was 1.49%at the rotational speed of 20 r/min.The maximum coefficient of variation of the fertilization stability was 2.86%at the rotational speed of 40 r/min,while the average fertilizer amount per lap for each distributor was 26.25 g/r.The results of the dynamic fertilization tests showed that the maximum relative error of the fertilizer distribution amount was 2.00%when the target fertilizer rates were 20,30,and 40 kg/667 m2.The results of the field tests showed that the average relative error of the fertilization amount was 3.53%,which satisfies the design standard.This research provides a reference for optimizing pneumatic fertilizer delivery systems and improving fertilization control systems and other pneumatic precision fertilizer application devices.

The dichotomous and incomplete adaptive immunity in COVID-19 patients with different disease severity

The adaptive immunity that protects patients from coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is not well characterized.In particular,the asymptomatic patients have been found to induce weak and transient SARS-CoV-2 antibody responses,but the underlying mechanisms remain unknown;meanwhile,the protective immunity that guide the recovery of these asymptomatic patients is elusive.Here,we characterized SARS-CoV-2-specific B-cell and T-cell responses in 10 asymptomatic patients and 64 patients with other disease severity(mild,n=10,moderate,n=32,severe,n=12)and found that asymptomatic or mild symptomatic patients failed to mount virus-specific germinal center(GC)B cell responses that result in robust and prolonged humoral immunity,assessed by GC response indicators including follicular helper T(TFH)cell and memory B cell responses as well as serum CXCL13 levels.Alternatively,these patients mounted potent virus-specific TH1 and CD8+T cell responses.In sharp contrast,patients of moderate or severe disease induced vigorous virus-specific GC B cell responses and associated TFH responses;however,the virus-specific TH1 and CD8+T cells were minimally induced in these patients.These results,therefore,uncovered the protective immunity in asymptomatic patients and also revealed the strikingly dichotomous and incomplete humoral and cellular immune responses in COVID-19 patients with different disease severity,providing important insights into rational design of effective COVID-19 vaccines.

Comfort evaluation and position parameter optimization of the steering wheel in agricultural machinery based on a three-level evaluation index system

The aim of this study is to evaluate the comfort and optimize the position parameters of steering wheel.Taking the H point of driver as the reference point,three position parameters of steering wheel were determined,which were used as experimental factors.A comprehensive evaluation index system of the comfort was established.The comfort range and optimal levels of three parameters were determined by a single factor test,based on which a response surface optimization and validation test was carried out.The optimization and validation test results show that the expected comprehensive score of the comfort is 0.864,and the average relative error between the predicted and the measured value is 4.18%,indicating that the optimization results are reliable.The findings can provide reference for the comfort optimization design of steering wheel in agricultural devices.

Disease severity dictates SARS-CoV-2-specific neutralizing antibody responses in COVID-19

COVID-19 patients exhibit differential disease severity after SARS-CoV-2 infection.It is currently unknown as to the correlation between the magnitude of neutralizing antibody(NAb)responses and the disease severity in COVID-19 patients.In a cohort of 59 recovered patients with disease severity including severe,moderate,mild,and asymptomatic,we observed the positive correlation between serum neutralizing capacity and disease severity,in particular,the highest NAb capacity in sera from the patients with severe disease,while a lack of ability of asymptomatic patients to mount competent NAbs.Furthermore,the compositions of NAb subtypes were also different between recovered patients with severe symptoms and with mild-tomoderate symptoms.These results reveal the tremendous heterogeneity of SARS-CoV-2-specific NAb responses and their correlations to disease severity,highlighting the needs of future vaccination in COVID-19 patients recovered from asymptomatic or mild illness.

Optimizing the adhesion of soil-touching parts based on biomimetic concepts using the Taguchi method

Several studies highlighted that the surface shape modifications which are inspired by the soil-burrowing animals have positively impacted the soil-tool adhesion;however,it is still unclear what the optimum dimensions of the domed surface are for minimizing soil-tool adhesion.In this study,twenty-seven domed discs were created according to Taguchi orthogonal array L27(3^(3))to determine the optimum dimensions of the domed surface that minimize normal adhesion force and disc sinkage simultaneously,as well as comparing the effect of the optimized domed disc versus the flat disc on the normal adhesion force under different soil conditions.The results revealed that disc coverage ratio and dome height to diameter ratio are essential to design parameters that influence the normal adhesion force of discs.According to the signal-to-noise ratio analysis,it was observed that the combination of 60%disc coverage ratio,25%dome height to diameter ratio,and 10 mm dome base diameter was found to be the most appropriate for the well-balanced improvement of both normal adhesion force and disc sinkage simultaneously.In all treatments,the optimized domed disc produced less normal adhesion force than the flat disc(about 7%-18%,according to soil condition).It can be concluded that properly designed domed surfaces can significantly reduce the normal adhesion force when compared to flat surfaces.

Characteristics of Suicides Caused by Drug Overdose in the State of Maryland

Suicidal drug overdose is a major public health issue.In the United States,every year more than 33,000 people commit suicides.Our study focused on the characteristics of suicide victims in the state of Maryland.Material and methods:This study was a retrospective review of autopsy cases of all suicide deaths caused by drug(s)or drug(s)with alcohol intoxication investigated by the OCME in Maryland over a 7-year period from January 2004 to December 2011.All deaths investigated by the OCME that require autopsy examination are subject to comprehensive toxicology testing for drugs and alcohol.The screen tests were performed using gas chromatography(GC)and radioimmunoassay techniques.All detected drugs and/or metabolites were confirmed using GC-mass spectrometry(GC-MS).Results:From 2004 to 2011,434 deaths were certified as suicide.Of the 434 suicidal overdose deaths,84%were white,11%were African-American,and about 5%were either Hispanic or Asian.The male and female ratio was almost equal.Their ages ranged 15-82 years.Of the 434 suicidal drug overdose deaths,277 victims(63.8%)consumed a single drug type and 157(36.2%)consumed more than one type of drug.Of the 277 single-drug overdose cases,71.1%suicides were due to prescription drugs,23.5%due to over-the-counter drugs,and 5.4%due to street/recreational drugs.Among single-type prescription drugs,analgesic(N=76),antidepressant(N=45),and neuroleptic(N=35)classes were the three leading type of drugs used in suicidal deaths.Oxycodone,morphine,quetiapine,and amitriptyline were the most common prescription drugs in suicidal overdose.Diphenhydramine was the leading over-the-counter drug.Of the 157 victims who consumed more than one drug,combined prescription drugs were present in 54.1%,mixed prescription and overthe-counter drugs in 29.3%,and prescription drugs/over-the-counter drugs and street drugs in 16.6%of cases.Of the multiple-drug overdose suicides,66.2%cases involved antidepressants.Discussion:Suicide is found in every age,racial,and ethnic group.In the USA,poisoning is thethird-leading method of suicide,following firearm injuries and hanging/strangulation.Our study indicates that prescription drugs,such as those in the opioid analgesic,neuroleptic,and antidepressant class(e.g.,oxycodone,morphine,quetiapine,amitriptyline,doxepin,and citalopram),are of special concern in the context of suicidal overdose deaths in the state of Maryland.Therefore,addressing the problems of intentional drug overdose in our society is worthy of public attention.

Evaluation of Medical Malpractice Litigations in China, 2002–2011

In the past 10 years,medical malpractice litigations have become a serious public health and social issues in China.This study aims to evaluate the medical malpractice litigations in Beijing.A retrospective study was conducted using the database from the Key Laboratory of Evidence Science(KLES)at China University of Political Science and Law(CUPL)to demonstrate the extent and characteristics of the alleged medical malpractice cases and the conclusions of forensic evaluation from 2002 to 2011 in Beijing.A total of 1515 alleged medical malpractice cases were investigated by forensic medical experts at KLES‑CUPL during the study period.Of the 1515 cases,45.2%were male patients and 54.8%were female patients.The age of the patients ranged from newborn to 90 years,with average age of 44 years.The alleged medical malpractice cases involved 11.3%of newborns.From 2002 to 2006,the average number of medical malpractice litigations was 123 cases per year,while from 2007 to 2011,the average number of medical malpractice litigations was 180 per year,a 46%increase.Of the 1515 cases,588(38.8%)litigations were due to the patients’death.Clinical departments involved in claims most often were obstetrics(18.9%),followed by orthopedics(15.1%)and general surgery(11.5%).Medical error was found in 945(62.4%)cases.Of the 945 confirmed medical malpractice cases,the top medical misadventures were diagnostic errors(36.5%),improper performance of procedures(36.4%),and medication error(12.6%).Although tertiary hospitals were more likely to be sued than the secondary and primary hospitals(63.6%vs.36.4%),there was no significant difference between tertiary and secondary hospitals in the percentage of confirmed malpractice cases(62.3%vs.59.4%).The primary hospitals had the highest confirmed malpractice cases(73.2%).