Antimicrobial indole alkaloids from Tabernaemontana corymbosa

Four unreported monoterpene indole alkaloids,tabernaecorymines B−E(1−4),together with twenty-one known indole alkaloids(5−25)were obtained from the stem bark of Tabernaemontana corymbosa.Their structures and absolute configurations were elucidated by extensive spectroscopy,quantum chemical calculations,DP4+probability analyses and Mo2(OAc)4-induced electronic circular dichroism experiment.The antibacterial and antifungal activities of these compounds were evaluated and some of them showed significant activity against Staphylococcus aureus,Bacillus subtilis,Streptococcus dysgalactiae and Candida albicans.

云南不同产地臭灵丹精油化学成分及抗菌活性(英文)

采用水蒸气蒸馏的方法,对采自云南4个不同地区(昆明,丽江,普洱,新平)的臭灵丹叶子进行挥发油提取,并利用GC-MS和GC-FID气相色谱联用技术分析其化学组成,分别从中鉴定出39、37、33、44个化合物。鉴定的化合物各占挥发油总量的93.32%(昆明)、80.57%(丽江)、92.24%(普洱)和93.85%(新平)。研究结果表明,采自这四个地区植物样品提取所得挥发油化学组成不尽相同:昆明产臭灵丹挥发油富含芳香族化合物(38.06%);而从丽江和普洱样品中获得的挥发油化学成分以倍半萜(分别为19.89%和44.65%)和氧化倍半萜(分别为40.85%和35.11%)为主;非氧化单萜和非氧化倍半萜化合物(分别为42.23%和33.25%)则是新平产臭灵丹挥发油的主要化合物类型。同时,对这些不同产地的臭灵丹挥发油也进行了抗菌活性测试。从以上实验研究结果推测环境差异是影响臭灵丹挥发油化学组成差异的重要因素,而不同的化学组成可能是导致4种样品抗菌敏感程度不同的原因。