The flavonoid compounds are important secondary metabolites with versatile human nutritive benefits and fulfill a multitude of functions during plant growth and development.The abundance of different flavonoid compoun...The flavonoid compounds are important secondary metabolites with versatile human nutritive benefits and fulfill a multitude of functions during plant growth and development.The abundance of different flavonoid compounds are finely tuned with species-specific pattern by a ternary MBW complex,which consists of a MYB,a bHLH,and a WD40 protein,but the essential role of SlAN11,which is a WD40 protein,is not fully understood in tomato until now.In this study,a tomato WD40 protein named as SlAN11 was characterized as an effective transcription regulator to promote plant anthocyanin and seed proanthocyanidin(PA)contents,with late flavonoid biosynthetic genes activated in 35S::SlAN11 transgenic lines,while the dihydroflavonol flow to the accumulation of flavonols or their glycosylated derivatives was reduced by repressing the expression of SlFLS in this SlAN11-overexpressed lines.The above changes were reversed in 35S::SlAN11-RNAi transgenic lines except remained levels of flavonol compounds and SlFLS expression.Interestingly,our data revealed that SlAN11 gene could affect seed dormancy by regulating the expressions of abscisic acid(ABA)signaling-related genes SlABI3 and SlABI5,and the sensitivity to ABA treatment in seed germination is conversely changed by SlAN11-overexpressed or-downregulated lines.Yeast two-hybrid assays demonstrated that SlAN11 interacted with bHLH but not with MYB proteins in the ternary MBW complex,whereas bHLH interacted with MYB in tomato.Our results indicated that low level of anthocyanins in tomato fruits,with low expression of bHLH(SlTT8)and MYB(SlANT1 and SlAN2)genes,remain unchanged upon modification of SlAN11 gene alone in the transgenic lines.These results suggest that the tomato WD40 protein SlAN11,coordinating with bHLH and MYB proteins,plays a crucial role in the fine adjustment of the flavonoid biosynthesis and seed dormancy in tomato.展开更多
In 2019,Ne Zha:I’m the Destiny won more than 5-billion-yuan box office in Chinese movie market.Although the box office is not the only criterion for judging the quality of an animated movie,Ne Zha breaks a certain st...In 2019,Ne Zha:I’m the Destiny won more than 5-billion-yuan box office in Chinese movie market.Although the box office is not the only criterion for judging the quality of an animated movie,Ne Zha breaks a certain stereotype in storytelling,technical means,and the setting of the positive and negative characters.This potentially indicates that in producing an animated film,Chinese artists start to pay more attention to how to refine a movie production,or how to make the story more interesting.One of the attractive elements of this movie is that,Ne Zha has made a breakthrough in creation of a character of Children’s hero,and is totally different from its previous versions:with his joy and sorrow,audiences see the characteristics of Ne Zha as a nice as well as an aggressive boy.This partially because in setting the role of Ne Zha,boundaries between the good and the evil or between beauty and ugly are not so clear in the animation,while it features that Chinese cartoons are set to focus more on authenticity rather than their instructive functions.Additionally,the change of the image of Ne Zha and the subversive characters re-created in the movie have metaphorical significances,featuring how“Chinese school”reconstructs the subjectivity of Chinese stories in contemporary world.展开更多
BACKGROUND Percutaneous vertebroplasty(PVP)has been widely used in osteoporotic vertebral compression fracture(OVCF).Following surgery,the bone cement would be positioned permanently.However,in some cases of lumbar de...BACKGROUND Percutaneous vertebroplasty(PVP)has been widely used in osteoporotic vertebral compression fracture(OVCF).Following surgery,the bone cement would be positioned permanently.However,in some cases of lumbar degenerative disease,the cemented vertebrae needs to be fixed after decompression and fusion procedure.It is difficult to implant traditional pedicle screws into the cemented vertebrae because of the bone cement filling.At present,the main treatment strategy is to skip the cemented vertebra and conduct a long segment fixation.This article presents a cortical bone trajectory(CBT)fixation technique for cemented vertebrae.CASE SUMMARY PVP involving the L3 and L4 was performed in an 82-year-old man due to OVCF.During the surgery,bone cement leakage occurred,resulting in compression of the root of the right L3 nerve.We performed a partial facetectomy to retrieve the leaked bone cement and to relieve the patient’s neurological symptoms.After 3 mo,the patient developed lumbar disc herniation in L3/4,potentially due to instability caused by the previous surgery.Therefore,it was necessary to perform intervertebral fusion and fixation.It was difficult to implant traditional trajectory pedicle screws in L3 and L4 because of the bone cement filling.Hence,we implanted CBT screws in the L3 and L4 vertebrae.As a result,the patient’s symptoms resolved and he reported satisfaction with the surgery at follow-up after 8 mo.CONCLUSION It is feasible to utilize CBT in cemented vertebrae for the treatment of lumbar degenerative disease.展开更多
As the only way for air to enter the lungs,the trachea plays an indispensable role in conducting,cleaning,and humidifying air.Tumors,trauma,and congenital disease of the trachea are serious threats to patients[1].Loca...As the only way for air to enter the lungs,the trachea plays an indispensable role in conducting,cleaning,and humidifying air.Tumors,trauma,and congenital disease of the trachea are serious threats to patients[1].Local tracheal disease can be treated with a balloon or stent,but for extensive tracheal disease,tracheal reconstruction after surgical resection is the only treatment[2–4].However,the tension of the anastomotic site also increases with expansion of the resection range,which causes a series of complications,such as poor anastomotic healing,anastomotic fistulae,and tracheal rupture,and even life-threatening complications in severe cases[5].展开更多
This paper is built on the study of one key blog-Wang Xiaofeng＇s No Guess （2006-2011） to explore how a Chinese intellectual uses his satirical skills in the blogosphere, how his blog reshapes the form of political ...This paper is built on the study of one key blog-Wang Xiaofeng＇s No Guess （2006-2011） to explore how a Chinese intellectual uses his satirical skills in the blogosphere, how his blog reshapes the form of political culture, and how the blogosphere through such interventions proceeds in the development of political communications in China. Two key issues are addressed in the paper. Firstly, drawing on the concept of ＂blogging culture＂, the author argues that blogging has potentially reconfigured political information around people＇s everyday lives, offering alternative modes of ＂public talk＂. The case of Wang Xiaofeng shows that satire can be used to make fun of the state, policies, and established ideologies, improving a previously restricted communicative environment toward more open. Secondly, the rise in the value placed on individualism in China, and the rise of peer-to-peer media mean that bloggers who pursue self-expression simultaneously through such self-expression. In other words, Wang Xiaofeng＇s No Guess blog demonstrates that individual opinions across the blogosphere have implicitly challenged political discourse; however, they always have to struggle with an ongoing censorship, negotiate an unstable discursive space and thus, can only enjoy a limited success.展开更多
News frames is a general application of the Frame Theory in journalistic practice,and the setting of the Frame Theory in news media,to some extent,may make the news agency have more choices of the topics,more channels...News frames is a general application of the Frame Theory in journalistic practice,and the setting of the Frame Theory in news media,to some extent,may make the news agency have more choices of the topics,more channels of the report,and more impacts on readers and audiences.It is for this reason that news media are very interested in setting up their news frame to guide their reportage.It won’t be surprised that when important affairs took place,the media set a theme for their coverage;while at the same time,audiences recognized that they are allowed to know the facts as well to evaluate the events properly.The coverage of disaster news is one of the concrete examples.However,when reading the reportage framework of the news in China,it can be seen that media would be likely to set similar frames for the focus of the report,and this potentially created complexity and difficulty in analyzing disaster news events in terms of content classification,reporting form,and news-making on effectiveness.The outbreak of the 2020 COVID-19 gathered media to work on a centralized proposal–anti-epidemic,so that textual,audio-visual contents and other forms of reporting show a diversified perspective for disaster news.This reporting form is a new challenge for Chinese news media,reflected in their practice on how Chinese government and people fought against the virus,how Chinese medical community dispatched their team to assist COVID-19 fight,and how Chinese media responded to the vilification of foreign media during that period.This paper takes three established media Hubei Daily,CCTV and China Daily as examples for an in-depth analysis.展开更多
Objective:To observe the ultrasonographic characteristics of conjoined tendon repair in direct anterior approach for total hip arthroplasty(DAA-THA),and to evaluate the efficacy of musculoskeletal ultrasound in determ...Objective:To observe the ultrasonographic characteristics of conjoined tendon repair in direct anterior approach for total hip arthroplasty(DAA-THA),and to evaluate the efficacy of musculoskeletal ultrasound in determining the healing after joint tendon repair.Methods:A total of 60 patients who required primary total hip arthroplasty in Yulin Orthopedic Hospital of Chinese and Western Medicine from July 2020 to July 2021 were selected;the patients were divided into two groups,an observation group,group A(n=30),and a control group,group B(n=30),according to different intraoperative methods.There was no significant difference in gender,age,and diagnosis between the two groups.Direct anterior approach was used for both the groups.For group A,the joint capsule and conjoined tendon(superior gemellus,obturator internus,and inferior gemellus)were repaired in situ,whereas for group B,only the joint capsule was repaired in situ,while the conjoined tendon was not repaired.The healing of the tendon was observed.Results:(1)in terms of diagnosis,after conjoined tendon repair,26 cases in group A showed good tendon continuity,good tension,and a small amount of effusion echo around,three cases showed partial interruption of tendon echo,low echo,or no echo inside with insufficient structural clarity,and a case showed complete interruption;in group B,all 30 cases had continuous interruption,poor tension,tendon retraction,and thickening;the healing rate of group A’s conjoined tendon repair was 96.67%;(2)in terms of prognostic assessment,one month after the surgery,the Harris score of group A was significantly higher than that of group B(P<0.05);however,there was no significant difference in the terms of the Harris score between the two groups 3-6 months after surgery(P>0.05);the effective tension of conjoined tendon and the effective muscle strength of group A were significantly higher than those of group B(P<0.05).Conclusion:Musculoskeletal ultrasound has high diagnostic value in the healing of conjoined tendon and provides dynamic clinical observation after conjoined tendon repair in DAA-THA;it is proven that DAA-THA with conjoined tendon repair on the premise of reconstructing the joint capsule can well restore its tension,enhance its muscle strength,significantly improve early joint stability and joint function,as well as facilitate the rapid recovery of patients.展开更多
目的:研究长期高脂饮食为主的肥胖个体的肠道细菌多样性,比较不同健康状态的肥胖者肠道菌群的差异。方法:对14个肥胖个体根据健康状况分为3组(健康组,H组;胃肠道疾病组,D组;慢性病组,T组),提取3组样品粪便细菌宏基因组DNA,扩增其16S r ...目的:研究长期高脂饮食为主的肥胖个体的肠道细菌多样性,比较不同健康状态的肥胖者肠道菌群的差异。方法:对14个肥胖个体根据健康状况分为3组(健康组,H组;胃肠道疾病组,D组;慢性病组,T组),提取3组样品粪便细菌宏基因组DNA,扩增其16S r RNA基因全长序列,利用三代测序技术测定样品宏基因组DNA的16S r RNA基因全长序列,采用相关生物信息学与统计学方法分析。结果:肥胖个体肠道细菌多样性降低,高丰度细菌含量增加,低丰度细菌含量减少,拟杆菌属是肥胖人群肠道细菌中含量最高的菌属。慢性病肥胖患者肠道菌群的B/F值(拟杆菌门/厚壁菌门)降低,同时产气柯林斯菌和Lachnospira peotinoschiza的相对含量显著高于健康组的肥胖个体(LDA>2)。此外,患有腹泻等胃肠道疾病的肥胖个体(D组)变形菌门下的一些致病菌相对含量增加。结论:肥胖个体的健康状态影响其肠道菌群结构和组成,肥胖引发的不同并发症可能与肠道菌群相关。展开更多
基金The research was supported by the National Natural Science Foundation of China(No.31500205,No.31770644,and No.81703656)Scientific Research Fund of Sichuan Provincial Education Department(No.17ZB0456 and No.13TD0023)+2 种基金Yunnan Engineering Laboratory for Agro-environment Pollution Control and Eco-remediation/The Innovation Team for Farmland Non-pollution Production of Yunnan Province(No.2017HC015)the doctoral Fund Project(No.14zx7157 and No.13zx7116)Longshan academic talent research supporting program(No.17LZXT09)of Southwest University of Science and Technology.
文摘The flavonoid compounds are important secondary metabolites with versatile human nutritive benefits and fulfill a multitude of functions during plant growth and development.The abundance of different flavonoid compounds are finely tuned with species-specific pattern by a ternary MBW complex,which consists of a MYB,a bHLH,and a WD40 protein,but the essential role of SlAN11,which is a WD40 protein,is not fully understood in tomato until now.In this study,a tomato WD40 protein named as SlAN11 was characterized as an effective transcription regulator to promote plant anthocyanin and seed proanthocyanidin(PA)contents,with late flavonoid biosynthetic genes activated in 35S::SlAN11 transgenic lines,while the dihydroflavonol flow to the accumulation of flavonols or their glycosylated derivatives was reduced by repressing the expression of SlFLS in this SlAN11-overexpressed lines.The above changes were reversed in 35S::SlAN11-RNAi transgenic lines except remained levels of flavonol compounds and SlFLS expression.Interestingly,our data revealed that SlAN11 gene could affect seed dormancy by regulating the expressions of abscisic acid(ABA)signaling-related genes SlABI3 and SlABI5,and the sensitivity to ABA treatment in seed germination is conversely changed by SlAN11-overexpressed or-downregulated lines.Yeast two-hybrid assays demonstrated that SlAN11 interacted with bHLH but not with MYB proteins in the ternary MBW complex,whereas bHLH interacted with MYB in tomato.Our results indicated that low level of anthocyanins in tomato fruits,with low expression of bHLH(SlTT8)and MYB(SlANT1 and SlAN2)genes,remain unchanged upon modification of SlAN11 gene alone in the transgenic lines.These results suggest that the tomato WD40 protein SlAN11,coordinating with bHLH and MYB proteins,plays a crucial role in the fine adjustment of the flavonoid biosynthesis and seed dormancy in tomato.
文摘In 2019,Ne Zha:I’m the Destiny won more than 5-billion-yuan box office in Chinese movie market.Although the box office is not the only criterion for judging the quality of an animated movie,Ne Zha breaks a certain stereotype in storytelling,technical means,and the setting of the positive and negative characters.This potentially indicates that in producing an animated film,Chinese artists start to pay more attention to how to refine a movie production,or how to make the story more interesting.One of the attractive elements of this movie is that,Ne Zha has made a breakthrough in creation of a character of Children’s hero,and is totally different from its previous versions:with his joy and sorrow,audiences see the characteristics of Ne Zha as a nice as well as an aggressive boy.This partially because in setting the role of Ne Zha,boundaries between the good and the evil or between beauty and ugly are not so clear in the animation,while it features that Chinese cartoons are set to focus more on authenticity rather than their instructive functions.Additionally,the change of the image of Ne Zha and the subversive characters re-created in the movie have metaphorical significances,featuring how“Chinese school”reconstructs the subjectivity of Chinese stories in contemporary world.
文摘BACKGROUND Percutaneous vertebroplasty(PVP)has been widely used in osteoporotic vertebral compression fracture(OVCF).Following surgery,the bone cement would be positioned permanently.However,in some cases of lumbar degenerative disease,the cemented vertebrae needs to be fixed after decompression and fusion procedure.It is difficult to implant traditional pedicle screws into the cemented vertebrae because of the bone cement filling.At present,the main treatment strategy is to skip the cemented vertebra and conduct a long segment fixation.This article presents a cortical bone trajectory(CBT)fixation technique for cemented vertebrae.CASE SUMMARY PVP involving the L3 and L4 was performed in an 82-year-old man due to OVCF.During the surgery,bone cement leakage occurred,resulting in compression of the root of the right L3 nerve.We performed a partial facetectomy to retrieve the leaked bone cement and to relieve the patient’s neurological symptoms.After 3 mo,the patient developed lumbar disc herniation in L3/4,potentially due to instability caused by the previous surgery.Therefore,it was necessary to perform intervertebral fusion and fixation.It was difficult to implant traditional trajectory pedicle screws in L3 and L4 because of the bone cement filling.Hence,we implanted CBT screws in the L3 and L4 vertebrae.As a result,the patient’s symptoms resolved and he reported satisfaction with the surgery at follow-up after 8 mo.CONCLUSION It is feasible to utilize CBT in cemented vertebrae for the treatment of lumbar degenerative disease.
基金the projects from the National Natural Science Foundation of China(Grant Nos.81770091,81970091,82000099)Shanghai Pulmonary Hospital InnovationTeam(Grant Nos.FKCX1906).
文摘As the only way for air to enter the lungs,the trachea plays an indispensable role in conducting,cleaning,and humidifying air.Tumors,trauma,and congenital disease of the trachea are serious threats to patients[1].Local tracheal disease can be treated with a balloon or stent,but for extensive tracheal disease,tracheal reconstruction after surgical resection is the only treatment[2–4].However,the tension of the anastomotic site also increases with expansion of the resection range,which causes a series of complications,such as poor anastomotic healing,anastomotic fistulae,and tracheal rupture,and even life-threatening complications in severe cases[5].
文摘This paper is built on the study of one key blog-Wang Xiaofeng＇s No Guess （2006-2011） to explore how a Chinese intellectual uses his satirical skills in the blogosphere, how his blog reshapes the form of political culture, and how the blogosphere through such interventions proceeds in the development of political communications in China. Two key issues are addressed in the paper. Firstly, drawing on the concept of ＂blogging culture＂, the author argues that blogging has potentially reconfigured political information around people＇s everyday lives, offering alternative modes of ＂public talk＂. The case of Wang Xiaofeng shows that satire can be used to make fun of the state, policies, and established ideologies, improving a previously restricted communicative environment toward more open. Secondly, the rise in the value placed on individualism in China, and the rise of peer-to-peer media mean that bloggers who pursue self-expression simultaneously through such self-expression. In other words, Wang Xiaofeng＇s No Guess blog demonstrates that individual opinions across the blogosphere have implicitly challenged political discourse; however, they always have to struggle with an ongoing censorship, negotiate an unstable discursive space and thus, can only enjoy a limited success.
文摘News frames is a general application of the Frame Theory in journalistic practice,and the setting of the Frame Theory in news media,to some extent,may make the news agency have more choices of the topics,more channels of the report,and more impacts on readers and audiences.It is for this reason that news media are very interested in setting up their news frame to guide their reportage.It won’t be surprised that when important affairs took place,the media set a theme for their coverage;while at the same time,audiences recognized that they are allowed to know the facts as well to evaluate the events properly.The coverage of disaster news is one of the concrete examples.However,when reading the reportage framework of the news in China,it can be seen that media would be likely to set similar frames for the focus of the report,and this potentially created complexity and difficulty in analyzing disaster news events in terms of content classification,reporting form,and news-making on effectiveness.The outbreak of the 2020 COVID-19 gathered media to work on a centralized proposal–anti-epidemic,so that textual,audio-visual contents and other forms of reporting show a diversified perspective for disaster news.This reporting form is a new challenge for Chinese news media,reflected in their practice on how Chinese government and people fought against the virus,how Chinese medical community dispatched their team to assist COVID-19 fight,and how Chinese media responded to the vilification of foreign media during that period.This paper takes three established media Hubei Daily,CCTV and China Daily as examples for an in-depth analysis.
文摘Objective:To observe the ultrasonographic characteristics of conjoined tendon repair in direct anterior approach for total hip arthroplasty(DAA-THA),and to evaluate the efficacy of musculoskeletal ultrasound in determining the healing after joint tendon repair.Methods:A total of 60 patients who required primary total hip arthroplasty in Yulin Orthopedic Hospital of Chinese and Western Medicine from July 2020 to July 2021 were selected;the patients were divided into two groups,an observation group,group A(n=30),and a control group,group B(n=30),according to different intraoperative methods.There was no significant difference in gender,age,and diagnosis between the two groups.Direct anterior approach was used for both the groups.For group A,the joint capsule and conjoined tendon(superior gemellus,obturator internus,and inferior gemellus)were repaired in situ,whereas for group B,only the joint capsule was repaired in situ,while the conjoined tendon was not repaired.The healing of the tendon was observed.Results:(1)in terms of diagnosis,after conjoined tendon repair,26 cases in group A showed good tendon continuity,good tension,and a small amount of effusion echo around,three cases showed partial interruption of tendon echo,low echo,or no echo inside with insufficient structural clarity,and a case showed complete interruption;in group B,all 30 cases had continuous interruption,poor tension,tendon retraction,and thickening;the healing rate of group A’s conjoined tendon repair was 96.67%;(2)in terms of prognostic assessment,one month after the surgery,the Harris score of group A was significantly higher than that of group B(P<0.05);however,there was no significant difference in the terms of the Harris score between the two groups 3-6 months after surgery(P>0.05);the effective tension of conjoined tendon and the effective muscle strength of group A were significantly higher than those of group B(P<0.05).Conclusion:Musculoskeletal ultrasound has high diagnostic value in the healing of conjoined tendon and provides dynamic clinical observation after conjoined tendon repair in DAA-THA;it is proven that DAA-THA with conjoined tendon repair on the premise of reconstructing the joint capsule can well restore its tension,enhance its muscle strength,significantly improve early joint stability and joint function,as well as facilitate the rapid recovery of patients.
文摘目的:研究长期高脂饮食为主的肥胖个体的肠道细菌多样性,比较不同健康状态的肥胖者肠道菌群的差异。方法:对14个肥胖个体根据健康状况分为3组(健康组,H组;胃肠道疾病组,D组;慢性病组,T组),提取3组样品粪便细菌宏基因组DNA,扩增其16S r RNA基因全长序列,利用三代测序技术测定样品宏基因组DNA的16S r RNA基因全长序列,采用相关生物信息学与统计学方法分析。结果:肥胖个体肠道细菌多样性降低,高丰度细菌含量增加,低丰度细菌含量减少,拟杆菌属是肥胖人群肠道细菌中含量最高的菌属。慢性病肥胖患者肠道菌群的B/F值(拟杆菌门/厚壁菌门)降低,同时产气柯林斯菌和Lachnospira peotinoschiza的相对含量显著高于健康组的肥胖个体(LDA>2)。此外,患有腹泻等胃肠道疾病的肥胖个体(D组)变形菌门下的一些致病菌相对含量增加。结论:肥胖个体的健康状态影响其肠道菌群结构和组成,肥胖引发的不同并发症可能与肠道菌群相关。
