Tetrahydrobiopterin(BH4)is an essential co-factor in the biosynthesis of monoamine neurotransmitters.A nano-Pt/Pd modified electrode as the electrochemical detector(ED)for high-performance liquid chromatography(HPLC)c...Tetrahydrobiopterin(BH4)is an essential co-factor in the biosynthesis of monoamine neurotransmitters.A nano-Pt/Pd modified electrode as the electrochemical detector(ED)for high-performance liquid chromatography(HPLC)coupled with microdialysis sampling,is used to explore the effect of administration of BH4 on the monoamine neurotransmitters in the rat striatum.The researches demonstrate that the contents of dopamine(DA),5-hydroxytryptamine(5-HT),5-hydroxyindoleacetic acid(5-HIAA)and homovanillic acid(HVA)increase significantly with the administration of BH4.The pharmaceutical kinetics is carried out to research into the time course of BH_(4) effect on the concentration of monoamine neurotransmitters in rat striatum,which pro-vides reliable data for pathology and pharmacology research on neuroscience.展开更多
基金the National Natural Science Foundation of China(Grant No.20175006)the Doctoral Programs Foundation of the Minis try of Education of China(Grant No.20030269014)the BAS Company of Japan.
文摘Tetrahydrobiopterin(BH4)is an essential co-factor in the biosynthesis of monoamine neurotransmitters.A nano-Pt/Pd modified electrode as the electrochemical detector(ED)for high-performance liquid chromatography(HPLC)coupled with microdialysis sampling,is used to explore the effect of administration of BH4 on the monoamine neurotransmitters in the rat striatum.The researches demonstrate that the contents of dopamine(DA),5-hydroxytryptamine(5-HT),5-hydroxyindoleacetic acid(5-HIAA)and homovanillic acid(HVA)increase significantly with the administration of BH4.The pharmaceutical kinetics is carried out to research into the time course of BH_(4) effect on the concentration of monoamine neurotransmitters in rat striatum,which pro-vides reliable data for pathology and pharmacology research on neuroscience.
