Small interfering RNA(siRNA) has been proved to be a powerful tool for silencing target gene in cells, raising the possibility that siRNA can be employed as a therapy for treating cancers and other genetic diseases. H...Small interfering RNA(siRNA) has been proved to be a powerful tool for silencing target gene in cells, raising the possibility that siRNA can be employed as a therapy for treating cancers and other genetic diseases. However, siRNA transfection has the limitation due to the difficulty in the delivery of siRNA to target cells and tissues. To explore an efficient biocompatible siRNA delivery system, cationic diphenylalanine vesicles(CDPVs) were constructed to transfer survivin siRNA to human ovarian cancer cells. The morphology of CDPVs was characterized by scanning electron microscopy(SEM) and the distribution of survivin siRNA was characterized by confocal laser scanning microscopy, which reveal that diphenylalanine and the survivin siRNA were successfully co-delivered. After co-incubation for 48 h, the CDPVs/siRNA exhibited enhanced tumor cell growth inhabitation and apoptosis inducted in human SK-OV-3 ovarian carcinoma cells. Overall, CDPVs is an efficient siRNA delivery system and has a promising prospect for cancer therapy.展开更多
Drug carrier materials need to possess good biological safety.Presently,most biosafety evaluation studies use rodent animal models,including rats and rabbits.However,the cost of raising these animals is relatively hig...Drug carrier materials need to possess good biological safety.Presently,most biosafety evaluation studies use rodent animal models,including rats and rabbits.However,the cost of raising these animals is relatively high and the experimental period is long.Caenorhabditis elegans(C.elegans)presents an ideal toxicological evaluation model due to its simple structure,easy cultivation,short life cycle,and evolutionary conservation.In this paper,we used C.elegans to test the biological safety of our pH-responsive carrier system(FFPFF self-assembling into a nanosphere structure,FFPFF Nps),which was designed for anti-tumor drug delivery.Our results showed that exposure to high doses of FFPFF Nps did not have a significant impact on the survival rate,growth,development,movement,and reproduction of C.elegans.The preliminary evaluation of the overall biological model of C.elegans shows that FFPFF Nps has good biological safety and warrants further study.展开更多
文摘Small interfering RNA(siRNA) has been proved to be a powerful tool for silencing target gene in cells, raising the possibility that siRNA can be employed as a therapy for treating cancers and other genetic diseases. However, siRNA transfection has the limitation due to the difficulty in the delivery of siRNA to target cells and tissues. To explore an efficient biocompatible siRNA delivery system, cationic diphenylalanine vesicles(CDPVs) were constructed to transfer survivin siRNA to human ovarian cancer cells. The morphology of CDPVs was characterized by scanning electron microscopy(SEM) and the distribution of survivin siRNA was characterized by confocal laser scanning microscopy, which reveal that diphenylalanine and the survivin siRNA were successfully co-delivered. After co-incubation for 48 h, the CDPVs/siRNA exhibited enhanced tumor cell growth inhabitation and apoptosis inducted in human SK-OV-3 ovarian carcinoma cells. Overall, CDPVs is an efficient siRNA delivery system and has a promising prospect for cancer therapy.
基金supported by the National Natural Science Foundation of China(No.31401086)the Science and Technology Development Program of Jilin Province,China(No.20200404114YY).
文摘Drug carrier materials need to possess good biological safety.Presently,most biosafety evaluation studies use rodent animal models,including rats and rabbits.However,the cost of raising these animals is relatively high and the experimental period is long.Caenorhabditis elegans(C.elegans)presents an ideal toxicological evaluation model due to its simple structure,easy cultivation,short life cycle,and evolutionary conservation.In this paper,we used C.elegans to test the biological safety of our pH-responsive carrier system(FFPFF self-assembling into a nanosphere structure,FFPFF Nps),which was designed for anti-tumor drug delivery.Our results showed that exposure to high doses of FFPFF Nps did not have a significant impact on the survival rate,growth,development,movement,and reproduction of C.elegans.The preliminary evaluation of the overall biological model of C.elegans shows that FFPFF Nps has good biological safety and warrants further study.
