HPLC法同时测定炒栀子配方颗粒中7种成分

目的建立HPLC法同时测定炒栀子配方颗粒中京尼平苷酸、去乙酰车叶草酸甲酯、绿原酸、栀子苷、芦丁、西红花苷Ⅰ、西红花苷Ⅱ的含量。方法该药物70%甲醇提取液的分析采用Agilent 5TC-C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1%甲酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长238、440 nm。结果7种成分在各自范围内线性关系良好(r>0.9995),平均加样回收率98.18%~99.45%,RSD 1.24%~2.68%。结论该方法准确简便,可操作性强,可用于炒栀子配方颗粒的质量控制。

葛根与粉葛HPLC特征图谱差异性研究

目的:研究葛根与粉葛特征图谱的差异性。方法:采用Agilent ZORBAX SB⁃C_(18)(250 mm×4.6 mm,5μm)色谱柱;以乙腈⁃0.2%冰醋酸溶液为流动相,梯度洗脱;流速为1.0 mL/min;检测波长为250 nm;柱温为30℃;进样量为10μL。利用“中药色谱指纹图谱相似度评价系统(2012版)”建立21批葛根与粉葛的特征图谱,并进行相似度评价及化学模式识别研究。结果:建立了葛根与粉葛的HPLC特征图谱,共确定15个共有峰,21批样品相似度为0.946~1.000,其中葛根样品相似度均>0.985。聚类分析和主成分分析均可用于区分葛根与粉葛。偏最小二乘判别分析结果显示大豆苷元、染料木素、葛根素、染料木苷等12个成分是引起差异的主要成分。结论:该研究建立的特征图谱方法可以区分葛根与粉葛,可为其品种的鉴别和质量控制提供参考。

败毒饮的高效液相指纹图谱及4种成分的含量测定

目的建立败毒饮的指纹图谱,并对4种成分的含量进行测定。方法采用安捷伦ZORBAX SB-C_(18)色谱柱(4.6 mm×150 mm,5 μm),以乙腈-0.1%磷酸为流动相进行梯度洗脱,检测波长334 nm,柱温30℃。通过相似度软件对11批败毒饮的指纹图谱进行质量评价,对指认的4个成分进行含量测定。与单味药进行比对,确定共有峰归属。结果建立了败毒饮的特征指纹图谱,得到16个共有峰,相似度在0.995以上,通过对照品指认出绿原酸、咖啡酸、黄芩苷、蒙花苷,其分别在19.92~199.2 ng(r^(2)=0.9994)、7.92~79.2 ng(r^(2)=0.9995)、248~2480 ng(r^(2)=0.9994)、78.16~781.6 ng(r^(2)=0.9995)与峰面积呈现良好的线性关系。结论所建立的方法简单,准确可靠,可用于败毒饮的质量控制。

A Dietary Supplement Jinghuosu Ameliorates Reproductive Damage Induced by Tripterygium Glycosides

Objective To determine the possible protective effects of Jinghuosu,a dietary supplement(DS),on tripterygium glycosides(TG)-induced reproductive system injury in rats and its underlying mechanisms.Methods A reproductive damage model was established in rats by feeding of TGs.Twenty-eight male Sprague Dawley rats were randomly divided into 4 groups using a random number table(n=7 in each):control(C)group,model(M)group,DS group and L-carnitine(LC)group.Rats in M,DS and LC groups received 40 mg/kg TGs orally.Starting from the 5th week,after administration of TGs for 4 h every day,rats in DS and LC groups were administered with 2.7 g/kg DS and 0.21 g/kg LC,respectively,for protective treatment over the next 4 weeks.Rats in Group C continued to receive the control treatment.Hematoxylin-eosin staining was used for histopathological analysis of rat testicular tissues.Enzyme-linked immunosorbent assay was performed to measure alkaline phosphatase(ALP),lactate dehydrogenase,alcohol dehydrogenase,total antioxidant capacity(T-AOC),superoxide dismutase,glutathione peroxidase(GSH-Px),and malondialdehyde(MDA)concentrations.Chemiluminescence assay was used to determine the serum testosterone content.Quantitative real-time PCR and Western blotting were conducted to analyze the expression of genes and proteins related to the testosterone synthesis pathway and the nuclear factor erythroid 2-related factor 2/heme oxygenase 1 antioxidant pathway.Results Oral administration of TGs induced significant increases in the testicular levels of zinc transporter 1 and MDA(P<0.05).On the other hand,sperm concentration,sperm motility,and serum testosterone,serum zinc,testicular zinc,Zrt-,Irt-like protein 1,ALP,luteinizing hormone(LH)receptor,steroidogenic acute regulatory protein,Cytochrome P450 family 11 subfamily A member 1,3β-hydroxysteroid dehydrogenase 1 T-AOC,GSH-Px,nuclear factor erythroid 2-related factor 2,heme oxygenase-1 and NAD(P)H:quinone oxidoreductase 1 levels decreased following TGs exposure(P<0.05).All of these phenotypes were evidently reversed by DS(P<0.05).Conclusion DS Jinghuosu protects against TG-induced reproductive system injury in rats,probably by improving zinc homeostasis,enhancing the testosterone synthesis and attenuating oxidative stress.

宫腔内人工授精妊娠结局的多因素分析

目的探讨宫腔内人工授精(IUI)妊娠结局的影响因素,为提高IUI妊娠率提供依据。方法分析2017年1月至2018年12月就诊于我院生殖中心接受IUI的患者共计534个周期。对不育类型、不孕年限、不育原因构成、男女双方年龄、男女双方体重指数(BMI)、精子浓度、前向运动精子百分率(PR)、精子DNA碎片(DFI)、精子自发顶体反应(AR)、处理后前向运动精子总数(PTMSC)、输卵管通畅程度、周期治疗方案、每周期排卵数、每周期授精次数、人工授精周期数等因素对妊娠结局的关系进行χ~2检验和Logistic回归分析。结果共纳入行IUI治疗的患者315例共计435个周期,总周期妊娠率为19.48%(104/534)。精子自发顶体反应:AR<10%的患者妊娠率(24.06%)高于AR≥10%的患者妊娠率(14.93%),差异有统计学意义(P=0.008);每周期排2~3个卵的患者妊娠率37.71%高于排1个卵患者的16.92%,差异具有统计学意义(P<0.001);精子自发AR<10%组妊娠几率是自发AR≥10%组的2.001倍(P=0.004);每周期排2~3个卵组妊娠几率是排1个卵组的4.148倍(P<0.001)。结论在IUI助孕治疗中,男方精子自发顶体反应率<10%,女方每周期排2~3成熟卵泡均可提高IUI妊娠率。