[ Objective] To develop a dot-ELISA （ dot-enzyme linked immunosorbent assay） method for detection of Salmonella in forage. [ Method] Forage samples including 100 finished feed samples, 85 fishmeal samples and 50 pro...[ Objective] To develop a dot-ELISA （ dot-enzyme linked immunosorbent assay） method for detection of Salmonella in forage. [ Method] Forage samples including 100 finished feed samples, 85 fishmeal samples and 50 protamine samples were randomly collected from a feed factory in Xining City, and Salmonella in these samples were detected by dot-ELISA. The Salmonella isolates were further identified using biochemical tests and serological tests. [Result] As detected by dot-ELISA, the positive rates of Salmonella in the finished feed, fishmeal and protamine were 38.00%, 62.35% and 60.00%, respectively. The coincidence rate between the dot-ELISA and routine identification method was 82.23%. [ Conclusion] The dot-ELISA has good specificity and sensitivity and is highly coincidental with the routine identification method. Therefore, dot-ELISA can be used for detection of Salmonella in forage.展开更多
文摘[ Objective] To develop a dot-ELISA （ dot-enzyme linked immunosorbent assay） method for detection of Salmonella in forage. [ Method] Forage samples including 100 finished feed samples, 85 fishmeal samples and 50 protamine samples were randomly collected from a feed factory in Xining City, and Salmonella in these samples were detected by dot-ELISA. The Salmonella isolates were further identified using biochemical tests and serological tests. [Result] As detected by dot-ELISA, the positive rates of Salmonella in the finished feed, fishmeal and protamine were 38.00%, 62.35% and 60.00%, respectively. The coincidence rate between the dot-ELISA and routine identification method was 82.23%. [ Conclusion] The dot-ELISA has good specificity and sensitivity and is highly coincidental with the routine identification method. Therefore, dot-ELISA can be used for detection of Salmonella in forage.
