前列地尔联合胰激肽原酶治疗早期糖尿病肾病的疗效及对炎性因子的影响

目的探讨前列地尔联合胰激肽原酶治疗早期糖尿病肾病的疗效及对炎性因子的影响。方法选取2017年1月至2018年4月磁县人民医院内分泌肾内科收治的80例早期糖尿病肾病患者为研究对象,按照治疗方法不同分为对照组和试验组,各40例。对照组采取饮食控制、适当运动、阿托伐他汀降血脂、氯沙坦钾降血压、注射胰岛素降血糖以及注射胰激肽原酶进行治疗;试验组在对照组的基础上给予前列地尔治疗。观察两组患者的临床疗效,治疗前后肾功能、空腹血糖、炎性因子[白细胞介素(IL)-6、肿瘤坏死因子α(TNF-α)、高敏C反应蛋白(hs-CRP)]的变化。结果试验组总有效率显著高于对照组[95.0%(38/40)比70.0%(28/40)](P<0.05)。试验组患者治疗后24 h尿蛋白排泄率、血肌酐、血尿素氮、血胱抑素C及空腹血糖均显著低于对照组[(88.0±24.3)mg比(112.6±36.6)mg、(44.8±6.4)μmol/L比(53.8±6.3)μmol/L、(4.5±1.2)mmol/L比(5.5±1.2)mmol/L、(0.7±0.3)mg/L比(1.2±0.4)mg/L、(6.3±0.7)mmol/L比(7.8±0.9)mmol/L](P<0.01)。试验组患者IL-6、TNF-α及hs-CRP均显著低于对照组[(35.7±13.0)ng/L比(69.2±12.6)ng/L、(17.5±14.2)ng/L比(24.7±14.0)ng/L、(3.7±1.2)mg/L比(6.2±1.4)mg/L](P<0.05或P<0.01)。结论前列地尔联合胰激肽原酶治疗早期糖尿病肾病临床疗效显著,可改善患者肾功能及空腹血糖情况,同时显著降低患者的血清炎性因子水平,延缓病情进展。

谷氨酸棒杆菌乳酰谷胱甘肽裂合酶基因克隆、表达及活性分析

乳酰谷胱甘肽裂合酶是生物体内降解丙酮醛的重要酶之一。食品级微生物谷氨酸棒杆菌ATCC13032基因组上的NCgl0106预测为乳酰谷胱甘肽裂合酶基因,但尚缺乏实验验证。本试验首先通过pCR技术扩增出预测的谷氨酸棒状杆菌ATCC13032乳酰谷胱甘肽裂合酶基因lac,并将之与表达载体pET-28a连接,转化BL21(DE3)感受态细胞,成功获得工程菌E.coli BL21(DE3)/pET-28a-lac。然后,使用IpTG诱导E.coli BL21(DE3)/pET-28a-lac表达重组蛋白Lac。SDS-PAGE试验结果表明:重组Lac蛋白在大肠杆菌进行了可溶性表达,其分子量约为14 kDa。最后对重组Lac蛋白进行了分离纯化、活性检测及酶学特性分析。应用His-tag纯化获得的重组蛋白溶液浓度为604.9μg/mL;活性检测显示重组Lac蛋白能催化丙酮醛和谷胱甘肽生成S-D-乳酰谷胱甘肽,表明谷氨酸棒杆菌lac基因是乳酰谷胱甘肽裂合酶基因;酶学特性分析表明重组乳酰谷胱甘肽裂合酶的最适反应温度为60℃,最适反应pH为7,金属离子K^+和Ca^(2+)对其活性略有增强作用,Ba^(2+)对酶活性几乎没有影响,Zn^(2+)、Mg^(2+)和Fe^(2+)对酶活性有一定的抑制作用,Co^(2+)和Mn^(2+)几乎完全抑制其活性,酶反应米氏常数K_m值为0.223 2 mmol/L,最大反应速率为0.025 mmol/(L·min)。

Mechanical Properties and Microstructure of Blended Cement Containing Modified Quartz Tailing

The amount of inert quartz tailing used in concrete construction is limited due to the low strength development of cementitious materials that may be caused by the quartz tailing. We manage to increase the strength of blended cement by modifying quartz tailing through solid-phase reaction of quartz tailing with carbide slag at high temperature. The mineral composition and morphology of the modified quartz tailing were examined by X-ray diffraction（XRD） and scanning electron microscopy（SEM）. The mechanical properties and microstructure of blended cement mortars containing modified quartz tailing were investigated. Results showed that the strengths of blended cement mortars containing modified quartz tailing were close to those of the corresponding blended cement mortars containing quartz tailing at early age of 3 d, but increased significantly to be similar to that of plain Portland cement mortars at late ages of 90 d. This is attributed to the microstructure densification and the enhancement of interface between quartz tailing and cement paste due to the hydration of b-C_2 S surface layer on modified quartz tailing.