背景和目的:2016年美国国家癌症研究所(National Cancer Institute,NCI)宣布不再使用NCI-60细胞系进行药物筛选,提示传统的肿瘤细胞系失去作为药物研发和基础研究工具的价值。NCI-60细胞“退休”原因是基于癌症细胞系和动物的实验结果...背景和目的:2016年美国国家癌症研究所(National Cancer Institute,NCI)宣布不再使用NCI-60细胞系进行药物筛选,提示传统的肿瘤细胞系失去作为药物研发和基础研究工具的价值。NCI-60细胞“退休”原因是基于癌症细胞系和动物的实验结果没有在临床试验中获得对应的预期,导致绝大部分潜在药物临床试验失败。癌症细胞系失去价值归因于肿瘤细胞经过长期培养后,其增殖和转移等主要生物学行为和与之有关的关键蛋白质系统发生了根本改变,已不能代表患者的真实癌症特征。现阶段需要创立一种来源于患者新鲜癌症组织和具有清晰临床背景的新癌症模型。本研究旨在为药物研发和基础研究建立经济的患者来源的可以无限传代的乳腺癌原代细胞系。方法:乳腺癌组织在贵州医科大学附属医院乳腺外科收集。肿瘤组织样本收集得到贵州医科大学附属医院伦理委员会批准(伦理编号:2022伦理第313号),收集和使用肿瘤组织均遵守赫尔辛基宣言,患者的乳腺癌组织消化分离后在BCMI培养基中培养,待乳腺癌细胞增殖到一定数量时更换成DMEM培养基。乳腺癌细胞经短串联重复序列(short tandem repeat,STR)检测确定细胞特异性遗传学标志和来源。克隆形成实验和动物实验分析乳腺癌原代细胞系形成肿瘤的能力。结果:成功建立了6种乳腺癌原代细胞系。他们具有清晰的临床病理学特征,包括病理学标志性分子检测、临床诊断、治疗方案和结果以及确定的预后结果。STR检测确定了6种乳腺癌原代细胞系特异性遗传标志和确定了该细胞系的来源。克隆形成实验和动物移植实验说明乳腺癌原代细胞系增殖能力显著大于传统乳腺癌细胞系,二者在形成肿瘤能力方面存在明显的差异。结论:构建的6种乳腺癌原代细胞系为乳腺癌药物研发和基础研究提供了新的癌症模型。展开更多
The Closeby Habitable Exoplanet Survey(CHES) mission is proposed to discover habitable-zone Earth-like planets of nearby solar-type stars(~10 pc away from our solar system) via microarcsecond relative astrometry.The m...The Closeby Habitable Exoplanet Survey(CHES) mission is proposed to discover habitable-zone Earth-like planets of nearby solar-type stars(~10 pc away from our solar system) via microarcsecond relative astrometry.The major scientific objectives of CHES are:to search for Earth Twins or terrestrial planets in habitable zones orbiting100 FGK nearby stars;further to conduct a comprehensive survey and extensively characterize nearby planetary systems.The primary payload is a high-quality,low-distortion,high-stability telescope.The optical subsystem is a coaxial three-mirror anastigmat(TMA) with a 1.2 m-aperture,0°.44 × 0°.44 field of view and 500 nm-900 nm working wave band.The camera focal plane is composed of a mosaic of 81 scientific CMOS detectors each with4 k × 4 k pixels.The heterodyne laser interferometric calibration technology is employed to ensure microarcsecond level(1 μas) relative astrometry precision to meet the requirements for detection of Earth-like planets.The CHES satellite operates at the Sun-Earth L2 point and observes all the target stars for 5 yr.CHES will offer the first direct measurements of true masses and inclinations of Earth Twins and super-Earths orbiting our neighbor stars based on microarcsecond astrometry from space.This will definitely enhance our understanding of the formation of diverse nearby planetary systems and the emergence of other worlds for solar-type stars,and finally provide insights to the evolution of our own solar system.展开更多
A first generation sodium Laser Guide Star Adaptive Optics System （LGS-AOS） was developed and integrated into the Lijiang 1.8 m telescope in 2013. The LGS-AOS has three sub-systems： （1） a 20W long pulsed sodium l...A first generation sodium Laser Guide Star Adaptive Optics System （LGS-AOS） was developed and integrated into the Lijiang 1.8 m telescope in 2013. The LGS-AOS has three sub-systems： （1） a 20W long pulsed sodium laser, （2） a 300-millimeter-diameter laser launch telescope, and （3） a 37-element com- pact adaptive optics system. On 2014 January 25, we obtained high resolution images of an my 8.18 star, HIP 43963, during the first light of the LGS-AOS. In this paper, the sodium laser, the laser launch telescope, the compact adaptive optics system and the first light results will be presented.展开更多
The Coude Echelle Spectrograph(CES)was originally mounted on Xinglong 2.16 m telescope in 1994.When the High Resolution Fiber-fed Spectrograph(HRS)was commissioned at Xinglong 2.16 m telescope,the red path of CES was ...The Coude Echelle Spectrograph(CES)was originally mounted on Xinglong 2.16 m telescope in 1994.When the High Resolution Fiber-fed Spectrograph(HRS)was commissioned at Xinglong 2.16 m telescope,the red path of CES was moved to Lijiang 1.8 m telescope,following some redesign and reinstallation.The CES of Lijiang 1.8 m telescope has the spectral resolution(R=λ/?λ)ranging from 20000 to 50000 corresponding to different slit widths.With a 2 k×2 k PI CCD,CES has a wavelength coverage between 570 nm to 1030 nm.In particular,the resolution of 37000 at 0.5 mm slit corresponds to 1.3′′on the sky.The limiting magnitude is V=11.5 with the use of the tip-tilt feedback system,and the S/N is about 40 for 1 hour exposure at 600 nm(R=37000).During the installation of CES,the tip-tilt mirror technology had successfully fulfilled high precision guiding and tracking for high resolution spectral observation and significantly improved the observation efficiency.展开更多
Fused magnesium aluminate spinel carbon materials were prepared using fused magnesium aluminate spinel and flake graphite as the main raw materials and phenolic resin as the binder.The effects of the Al-Si alloy addit...Fused magnesium aluminate spinel carbon materials were prepared using fused magnesium aluminate spinel and flake graphite as the main raw materials and phenolic resin as the binder.The effects of the Al-Si alloy addition(0,2%,4%,6%,8%and 10%,by mass)on the properties of fused magnesium aluminate spinel carbon materials were studied.The results show that fiber materials are generated in the samples with Al-Si alloy addition,which can significantly improve the cold and hot modulus of rupture,the cold elastic modulus,and the thermal shock resistance of the samples.When the addition of Al-Si alloy is 6%-8%,the comprehensive performance of the sample is the optimum.展开更多
Polyhalogenated carbazoles(PHCZs)are recently raising much attention due to their toxicity and ubiquitous environmental distribution.However,little knowledge is known about their ambient occurrences and the potential ...Polyhalogenated carbazoles(PHCZs)are recently raising much attention due to their toxicity and ubiquitous environmental distribution.However,little knowledge is known about their ambient occurrences and the potential source.In this study,we developed an analytical method based on GC-MS/MS to simultaneously determine 11 PHCZs in PM_(2.5) from urban Beijing,China.The optimized method provided low method limit of quantifications(MLOQs,1.45–7.39 fg/m^(3))and satisfied recoveries(73.4%–109.5%).This method was applied to analyze the PHCZs in the outdoor PM_(2.5)(n=46)and fly ash(n=6)collected from 3 kinds of surrounding incinerator plants(steel plant,medical waste incinerator and domestic waste incinerator).The levels of Σ_(11)PHCZs in PM_(2.5) ranged from 0.117 to 5.54 pg/m^(3)(median 1.18 pg/m^(3)).3-chloro-9H-carbazole(3-CCZ),3-bromo-9H-carbazole(3-BCZ),and 3,6-dichloro-9Hcarbazole(36-CCZ)were the dominant compounds,accounting for 93%.3-CCZ and 3-BCZ were significantly higher in winter due to the high PM_(2.5) concentration,while 36-CCZ was higher in spring,which may be related to the resuspending of surface soil.Furthermore,the levels of11PHCZs in fly ash ranged from 338 to 6101 pg/g.3-CCZ,3-BCZ and 36-CCZ accounted for 86.0%.The congener profiles of PHCZs between fly ash and PM_(2.5) were highly similar,indicating that combustion process could be an important source of ambient PHCZs.To the best of our knowledge,this is the first research providing the occurrences of PHCZs in outdoor PM_(2.5).展开更多
Large-aperture solar telescopes play an important role in solar observations and research,and require high temporal and spatial resolution[1].To solve some fundamental problems such as the solar dynamo,coronal heating...Large-aperture solar telescopes play an important role in solar observations and research,and require high temporal and spatial resolution[1].To solve some fundamental problems such as the solar dynamo,coronal heating,and the triggering of major solar eruptions,the spatial resolution for solar-atmosphere observation should reach at least 0.1 arcsec[2].展开更多
The precise alignment of a high-performance telescope is a key factor to ensure the imaging quality.However,for telescopes with a wide field of view,the images are sometimes under-sampled.To study the effects of under...The precise alignment of a high-performance telescope is a key factor to ensure the imaging quality.However,for telescopes with a wide field of view,the images are sometimes under-sampled.To study the effects of undersampled images on the precision of telescope alignment,numerical simulations are implemented with the stochastic parallel gradient descent algorithm.The results show that the alignment program can converge stably and quickly.However,with the reduction of the full width at half-maximum of images,the relative residual errors increase from 9.5%to 19.5%,and the wavefront errors raise from 0.0972λto 0.1074λ,indicating that the accuracy of the alignment decreases.展开更多
文摘背景和目的:2016年美国国家癌症研究所(National Cancer Institute,NCI)宣布不再使用NCI-60细胞系进行药物筛选,提示传统的肿瘤细胞系失去作为药物研发和基础研究工具的价值。NCI-60细胞“退休”原因是基于癌症细胞系和动物的实验结果没有在临床试验中获得对应的预期,导致绝大部分潜在药物临床试验失败。癌症细胞系失去价值归因于肿瘤细胞经过长期培养后,其增殖和转移等主要生物学行为和与之有关的关键蛋白质系统发生了根本改变,已不能代表患者的真实癌症特征。现阶段需要创立一种来源于患者新鲜癌症组织和具有清晰临床背景的新癌症模型。本研究旨在为药物研发和基础研究建立经济的患者来源的可以无限传代的乳腺癌原代细胞系。方法:乳腺癌组织在贵州医科大学附属医院乳腺外科收集。肿瘤组织样本收集得到贵州医科大学附属医院伦理委员会批准(伦理编号:2022伦理第313号),收集和使用肿瘤组织均遵守赫尔辛基宣言,患者的乳腺癌组织消化分离后在BCMI培养基中培养,待乳腺癌细胞增殖到一定数量时更换成DMEM培养基。乳腺癌细胞经短串联重复序列(short tandem repeat,STR)检测确定细胞特异性遗传学标志和来源。克隆形成实验和动物实验分析乳腺癌原代细胞系形成肿瘤的能力。结果:成功建立了6种乳腺癌原代细胞系。他们具有清晰的临床病理学特征,包括病理学标志性分子检测、临床诊断、治疗方案和结果以及确定的预后结果。STR检测确定了6种乳腺癌原代细胞系特异性遗传标志和确定了该细胞系的来源。克隆形成实验和动物移植实验说明乳腺癌原代细胞系增殖能力显著大于传统乳腺癌细胞系,二者在形成肿瘤能力方面存在明显的差异。结论:构建的6种乳腺癌原代细胞系为乳腺癌药物研发和基础研究提供了新的癌症模型。
基金financially supported by the Strategic Priority Research Program on Space Science of the Chinese Academy of Sciences (Grant No. XDA 15020800)the National Natural Science Foundation of China (Grant Nos. 12033010, 41604152 and U1938111)Foundation of Minor Planets of the Purple Mountain Observatory and Youth Innovation Promotion Association CAS (Grant No. 2018178)。
文摘The Closeby Habitable Exoplanet Survey(CHES) mission is proposed to discover habitable-zone Earth-like planets of nearby solar-type stars(~10 pc away from our solar system) via microarcsecond relative astrometry.The major scientific objectives of CHES are:to search for Earth Twins or terrestrial planets in habitable zones orbiting100 FGK nearby stars;further to conduct a comprehensive survey and extensively characterize nearby planetary systems.The primary payload is a high-quality,low-distortion,high-stability telescope.The optical subsystem is a coaxial three-mirror anastigmat(TMA) with a 1.2 m-aperture,0°.44 × 0°.44 field of view and 500 nm-900 nm working wave band.The camera focal plane is composed of a mosaic of 81 scientific CMOS detectors each with4 k × 4 k pixels.The heterodyne laser interferometric calibration technology is employed to ensure microarcsecond level(1 μas) relative astrometry precision to meet the requirements for detection of Earth-like planets.The CHES satellite operates at the Sun-Earth L2 point and observes all the target stars for 5 yr.CHES will offer the first direct measurements of true masses and inclinations of Earth Twins and super-Earths orbiting our neighbor stars based on microarcsecond astrometry from space.This will definitely enhance our understanding of the formation of diverse nearby planetary systems and the emergence of other worlds for solar-type stars,and finally provide insights to the evolution of our own solar system.
基金supported by the Creative Foundation of the Chinese Academy of Sciences,China
文摘A first generation sodium Laser Guide Star Adaptive Optics System （LGS-AOS） was developed and integrated into the Lijiang 1.8 m telescope in 2013. The LGS-AOS has three sub-systems： （1） a 20W long pulsed sodium laser, （2） a 300-millimeter-diameter laser launch telescope, and （3） a 37-element com- pact adaptive optics system. On 2014 January 25, we obtained high resolution images of an my 8.18 star, HIP 43963, during the first light of the LGS-AOS. In this paper, the sodium laser, the laser launch telescope, the compact adaptive optics system and the first light results will be presented.
基金supported by the National Natural Science Foundation of China(11603072,11727806 and 11573069)the National Science Foundation of Yunnan(2016FA001)the CAS“Light of West China” Program and the Youth Innovation Promotion Association of the CAS.
文摘The Coude Echelle Spectrograph(CES)was originally mounted on Xinglong 2.16 m telescope in 1994.When the High Resolution Fiber-fed Spectrograph(HRS)was commissioned at Xinglong 2.16 m telescope,the red path of CES was moved to Lijiang 1.8 m telescope,following some redesign and reinstallation.The CES of Lijiang 1.8 m telescope has the spectral resolution(R=λ/?λ)ranging from 20000 to 50000 corresponding to different slit widths.With a 2 k×2 k PI CCD,CES has a wavelength coverage between 570 nm to 1030 nm.In particular,the resolution of 37000 at 0.5 mm slit corresponds to 1.3′′on the sky.The limiting magnitude is V=11.5 with the use of the tip-tilt feedback system,and the S/N is about 40 for 1 hour exposure at 600 nm(R=37000).During the installation of CES,the tip-tilt mirror technology had successfully fulfilled high precision guiding and tracking for high resolution spectral observation and significantly improved the observation efficiency.
基金National Key Research and Development Program of China(2021YFB3701404)National Natural Science Foundation of China(51932008)for the support of the work
文摘Fused magnesium aluminate spinel carbon materials were prepared using fused magnesium aluminate spinel and flake graphite as the main raw materials and phenolic resin as the binder.The effects of the Al-Si alloy addition(0,2%,4%,6%,8%and 10%,by mass)on the properties of fused magnesium aluminate spinel carbon materials were studied.The results show that fiber materials are generated in the samples with Al-Si alloy addition,which can significantly improve the cold and hot modulus of rupture,the cold elastic modulus,and the thermal shock resistance of the samples.When the addition of Al-Si alloy is 6%-8%,the comprehensive performance of the sample is the optimum.
基金supported by the National Key Research and Development Program of China(Nos.2018YFA0901104 and 2019YFE0111100)the National Natural Science Foundation of China(No.91743206)the Special Project of Eco-Environmental Technology for Peak Carbon Dioxide Emissions and Carbon Neutrality(No.RCEES-TDZ-2021-23).
文摘Polyhalogenated carbazoles(PHCZs)are recently raising much attention due to their toxicity and ubiquitous environmental distribution.However,little knowledge is known about their ambient occurrences and the potential source.In this study,we developed an analytical method based on GC-MS/MS to simultaneously determine 11 PHCZs in PM_(2.5) from urban Beijing,China.The optimized method provided low method limit of quantifications(MLOQs,1.45–7.39 fg/m^(3))and satisfied recoveries(73.4%–109.5%).This method was applied to analyze the PHCZs in the outdoor PM_(2.5)(n=46)and fly ash(n=6)collected from 3 kinds of surrounding incinerator plants(steel plant,medical waste incinerator and domestic waste incinerator).The levels of Σ_(11)PHCZs in PM_(2.5) ranged from 0.117 to 5.54 pg/m^(3)(median 1.18 pg/m^(3)).3-chloro-9H-carbazole(3-CCZ),3-bromo-9H-carbazole(3-BCZ),and 3,6-dichloro-9Hcarbazole(36-CCZ)were the dominant compounds,accounting for 93%.3-CCZ and 3-BCZ were significantly higher in winter due to the high PM_(2.5) concentration,while 36-CCZ was higher in spring,which may be related to the resuspending of surface soil.Furthermore,the levels of11PHCZs in fly ash ranged from 338 to 6101 pg/g.3-CCZ,3-BCZ and 36-CCZ accounted for 86.0%.The congener profiles of PHCZs between fly ash and PM_(2.5) were highly similar,indicating that combustion process could be an important source of ambient PHCZs.To the best of our knowledge,this is the first research providing the occurrences of PHCZs in outdoor PM_(2.5).
基金supported by the National Natural Science Foundation of China(Grant No.11727805)the Youth Innovation Promotion Association,Chinese Academy of Sciences(Grant No.2018412)。
文摘Large-aperture solar telescopes play an important role in solar observations and research,and require high temporal and spatial resolution[1].To solve some fundamental problems such as the solar dynamo,coronal heating,and the triggering of major solar eruptions,the spatial resolution for solar-atmosphere observation should reach at least 0.1 arcsec[2].
基金supported by the National Natural Science Foundation of China(No.11873008)
文摘The precise alignment of a high-performance telescope is a key factor to ensure the imaging quality.However,for telescopes with a wide field of view,the images are sometimes under-sampled.To study the effects of undersampled images on the precision of telescope alignment,numerical simulations are implemented with the stochastic parallel gradient descent algorithm.The results show that the alignment program can converge stably and quickly.However,with the reduction of the full width at half-maximum of images,the relative residual errors increase from 9.5%to 19.5%,and the wavefront errors raise from 0.0972λto 0.1074λ,indicating that the accuracy of the alignment decreases.
