[ Objective] To investigate the optimal culture system for in vitro fertilization （IVF） of oocyte in bovine. [ Method] The IVF of mature bovine oocytes was conducted and the fertilized eggs were cultured in vitro wi...[ Objective] To investigate the optimal culture system for in vitro fertilization （IVF） of oocyte in bovine. [ Method] The IVF of mature bovine oocytes was conducted and the fertilized eggs were cultured in vitro with different culture systems. In the traditional culture system, the TALP medium and BO medium were used as fertilization medium, respectively; and M-199 medium supplemented with 5% FCS was used as fertilized egg culture medium. In the transitional culture system, the TALP medium and BO medium were used as fertilization medium; 60% TALP and 40% M-199 supplemented with 5% FCS and 60% BO and 40% M-199 supplemented with 5% FCS were used as fertilized egg culture medium, respectively. The effects of different culture systems on fertilization rate and cleavage rate were observed. E Result] In the transitional culture system （with TALP medium as fertilization medium and with 60% TALP and 40% M-199 supplemented with 5% FCS as fertilized egg culture medium）, the fertilization rate and cleavage rate were increased to 77.8% and 55.6%, respectively. [ Conclusion] The transitional culture system （with TALP medium as fertilization medium and with 60% TALP and 40% M-199 supplemented with 5% FCS as fertilized egg culture medium） is the optimal culture system for IVF of bovine oocyte.展开更多
Background Cancer of the esophagus and gastroesophageal junction remains a virulent malignancy with poor prognosis. Rapid progresses were made in chemotherapeutic agents and the development of molecular markers allowe...Background Cancer of the esophagus and gastroesophageal junction remains a virulent malignancy with poor prognosis. Rapid progresses were made in chemotherapeutic agents and the development of molecular markers allowed better identification of candidates for targeted therapy. This study aimed to identify the candidate peptides used for anti-angiogenic therapy of esophageal cancer by in vivo screening C7C peptide library for peptides binding specifically to blood vessels of human esophageal cancer.Methods The phage displayed C7C peptide library was injected intravenously into mice bearing human esophageal tumor xenografts under renal capsule. After 5 rounds of screening, 13 clones were picked up individually and sequenced.During each round of screening, titers of phage recovery were calculated from tumor xenograft and control tissues.Homing of these 9 peptides to tumor vessel was detected by calculating phage titers in the tumor xenograft and control tissues （lung and spleen） after each phage was injected into mice model, and compared with the distribution of phage M13 and Ⅷ-related antigen in tumor xenograft by immunohistochemical staining. Comparisons among groups of data were made using one-way analysis of variance （ANOVA）, followed by the Bonferroni multiple comparisons test.Results The number of phage recovered from tumor tissue of each round increased gradually in tumor group while decreased in control groups （P 〈0.01 in tumor and spleen, P 〈0.05 in lung）. Immunohistochemical staining showed similar staining pattern with M13 antibody or Ⅷ-related antigen antibody, suggesting that phages displaying the selected peptides could home to blood vessel of human esophageal cancer. According to their DNA, 9 corresponding peptide sequences were deduced. And the homing ability to blood vessel of phages displaying the selected peptides was confirmed by comparing with their recovery in tumor and control tissues. Two motifs, YSXNXW and PXNXXN, were also obtained by analyzing the homology of these peptide sequences. The staining distribution of phage with the sequence of PNPNNST was similar to that of the blood vessel marker factor Ⅷ-related antigen staining. After sequencing, each phage with the selected peptide of PNPNNST with 1.0×1011 pfu/ml was injected intravenously into mice. The homing ability to tumor vessel of these 9 kinds of peptides in the xenograft was higher than control tissues （lung and spleen）.Conclusion Nine peptides obtained from in vivo screening homed to the blood vessel of human esophageal cancer,and the two motifs of YSXNXW and PXNXXN are the possible biochemical recognition units binding to vascular endothelial cells of esophageal cancer.展开更多
基金supported by the Scientific Research & Development Fund of Tianjin Agricultural University
文摘[ Objective] To investigate the optimal culture system for in vitro fertilization （IVF） of oocyte in bovine. [ Method] The IVF of mature bovine oocytes was conducted and the fertilized eggs were cultured in vitro with different culture systems. In the traditional culture system, the TALP medium and BO medium were used as fertilization medium, respectively; and M-199 medium supplemented with 5% FCS was used as fertilized egg culture medium. In the transitional culture system, the TALP medium and BO medium were used as fertilization medium; 60% TALP and 40% M-199 supplemented with 5% FCS and 60% BO and 40% M-199 supplemented with 5% FCS were used as fertilized egg culture medium, respectively. The effects of different culture systems on fertilization rate and cleavage rate were observed. E Result] In the transitional culture system （with TALP medium as fertilization medium and with 60% TALP and 40% M-199 supplemented with 5% FCS as fertilized egg culture medium）, the fertilization rate and cleavage rate were increased to 77.8% and 55.6%, respectively. [ Conclusion] The transitional culture system （with TALP medium as fertilization medium and with 60% TALP and 40% M-199 supplemented with 5% FCS as fertilized egg culture medium） is the optimal culture system for IVF of bovine oocyte.
文摘Background Cancer of the esophagus and gastroesophageal junction remains a virulent malignancy with poor prognosis. Rapid progresses were made in chemotherapeutic agents and the development of molecular markers allowed better identification of candidates for targeted therapy. This study aimed to identify the candidate peptides used for anti-angiogenic therapy of esophageal cancer by in vivo screening C7C peptide library for peptides binding specifically to blood vessels of human esophageal cancer.Methods The phage displayed C7C peptide library was injected intravenously into mice bearing human esophageal tumor xenografts under renal capsule. After 5 rounds of screening, 13 clones were picked up individually and sequenced.During each round of screening, titers of phage recovery were calculated from tumor xenograft and control tissues.Homing of these 9 peptides to tumor vessel was detected by calculating phage titers in the tumor xenograft and control tissues （lung and spleen） after each phage was injected into mice model, and compared with the distribution of phage M13 and Ⅷ-related antigen in tumor xenograft by immunohistochemical staining. Comparisons among groups of data were made using one-way analysis of variance （ANOVA）, followed by the Bonferroni multiple comparisons test.Results The number of phage recovered from tumor tissue of each round increased gradually in tumor group while decreased in control groups （P 〈0.01 in tumor and spleen, P 〈0.05 in lung）. Immunohistochemical staining showed similar staining pattern with M13 antibody or Ⅷ-related antigen antibody, suggesting that phages displaying the selected peptides could home to blood vessel of human esophageal cancer. According to their DNA, 9 corresponding peptide sequences were deduced. And the homing ability to blood vessel of phages displaying the selected peptides was confirmed by comparing with their recovery in tumor and control tissues. Two motifs, YSXNXW and PXNXXN, were also obtained by analyzing the homology of these peptide sequences. The staining distribution of phage with the sequence of PNPNNST was similar to that of the blood vessel marker factor Ⅷ-related antigen staining. After sequencing, each phage with the selected peptide of PNPNNST with 1.0×1011 pfu/ml was injected intravenously into mice. The homing ability to tumor vessel of these 9 kinds of peptides in the xenograft was higher than control tissues （lung and spleen）.Conclusion Nine peptides obtained from in vivo screening homed to the blood vessel of human esophageal cancer,and the two motifs of YSXNXW and PXNXXN are the possible biochemical recognition units binding to vascular endothelial cells of esophageal cancer.
