阿戈美拉汀在精神专科医院住院患者中的临床应用分析

目的:调查阿戈美拉汀在精神专科医院住院患者中的临床使用情况,为临床合理用药提供参考。方法:调查本院2016年9月~2020年8月住院患者阿戈美拉汀的使用情况,统计分析阿戈美拉汀的使用率及用药合理性:超说明书使用情况、是否按照规范使用前检查肝功能、是否规律复查肝功能、血生化异常发生率,以及血生化异常病例的处理情况和治疗转归。结果:阿戈美拉汀使用率为1.23%,59.02%超适应症用药,4.10%超年龄使用,60.33%使用前即明确肝功能,77.91%住院期间规律复查肝功能,92.30%合并使用其他经肝脏代谢的药物。住院期间血生化异常的发生率为13.48%,药物性肝损伤(DILI)发生率为1.48%。结论:阿戈美拉汀存在超说明书用药。用药前应检查肝功能,用药后应规律复查功能,同时加强相关用药教育。

Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae pv.oryzae and nitrogen deficiency

Bacterial blight of rice caused by Xanthomonas oryzae pv.oryzae（Xoo） is one of high nitrogen（N） responsive diseases.Rice plants became more disease resistant with decreasing N suggesting that the crosstalk between disease resistance and N utilization pathways might exist.However,the co-regulatory components in such crosstalk have not been elucidated.Here,we comparatively analyzed the gene expression profiling of rice under Xoo inoculation,low N treatment,or a combination of both stresses,and identified the differentially-expressed genes（DEGs） in overlapping responses.These DEGs were involved in different biological processes,including innate immunity and nitrogen metabolism.The randomly-selected DEGs expression was validated by quantitative real-time PCR assays.Temporal expression of six genes from different functional categories suggested that N condition was the dominant factor when both stresses were present.These DEGs identified provide novel insights into the coordinated regulatory mechanism in biotic and abiotic stress responses in rice.

响应面法优化蛇莓花色苷浸提工艺及稳定性研究

目的用响应面法优化乙醇浸提蛇莓花色苷的工艺条件并研究其稳定性。方法在单因素试验基础上,选取乙醇体积分数、液料比、提取时间、提取温度为自变量,花色苷提取率为响应值,采用Box-Behnken设计试验及响应面法分析,优化浸提工艺。研究pH值、温度、有机酸、氧化剂对花色苷稳定性的影响。结果优化的最佳工艺为乙醇体积分数80%,液料比10∶1,提取时间93 min,提取温度36℃,在该最优条件下得到花色苷提取率为1.126%。稳定性实验表明蛇莓花色苷在酸性、低温条件下稳定性好,添加柠檬酸和没食子酸可增强其稳定性,而在氨基乙酸、H2O2中稳定性差。结论响应面法优化所得工艺科学合理、切实可行,可用于蛇莓花色苷的提取;对蛇莓花色苷操作时应注意条件以保持其稳定性。