病例女,53岁,因"咳嗽、胸部震痛4周,伴痰中带血丝及咳粉红色泡沫痰3周"入院。院外抗炎治疗无效。查体:体温36.9℃,血压116/62 mm Hg(1 mm Hg≈0.133 k Pa),心率为77次/min,心尖区可闻及舒张期杂音,双下肢轻度凹陷性水肿。实...病例女,53岁,因"咳嗽、胸部震痛4周,伴痰中带血丝及咳粉红色泡沫痰3周"入院。院外抗炎治疗无效。查体:体温36.9℃,血压116/62 mm Hg(1 mm Hg≈0.133 k Pa),心率为77次/min,心尖区可闻及舒张期杂音,双下肢轻度凹陷性水肿。实验室检查:心肌酶谱正常,D-二聚体1049 ug/L(参考值0~450 ug/L)。胸部CT平扫:两肺小叶间隔轻度增厚(图1);心脏略增大,二尖瓣区见低密度肿块影(图2),CT值约为32 HU;心包以及双侧胸腔内弧形液性低密度影。展开更多
In order to improve the pectin-degrading efficiency in wet processing of Arabica coffee in Yunnan, Box-Behnken design and single factor experiment were used to optimize the fermentation conditions of five pectinolytic...In order to improve the pectin-degrading efficiency in wet processing of Arabica coffee in Yunnan, Box-Behnken design and single factor experiment were used to optimize the fermentation conditions of five pectinolytic Wickerhamomyces anomalus strains from the fermentation broth of Arabica coffee in Baoshan, Yunnan during wet processing with pectase activity as an indicator. The results showed that the five strains all synthesized pectin lyase(PL), polygalacturonase(PG), and pectin methylesterase(PM).Among them, strain CAP5 had strong ability to produce PG and PL,while strain CAP4 secreted a large amount of PM. Under optimized conditions, the activity of PG, PL, and PM of the five strains came in at 250.17~411.20 U/mL, 12.98~16.55 U/mL, and 208.52~322.83 U/mL,respectively. The four factors of nitrogen source concentration,fermentation time, Mn2+ concentration, and pH value were optimized and the optimal pectinase-producing fermentation conditions for five strains were as follows: peptone 2.2 g/L, fermentation time 30 h, Mn2+ 1.5 mmol/L, and pH 4.3. After fermentation under the optimized conditions, the maximum PG activity of CAP5 amounted to 411.20 U/mL, 114.03% higher than that before optimization.Meanwhile, the PG activity of strains CAP3, CAP4, CAP8, and CAP10 increased by 86.74%, 114.55%, 65.79%, and 66.07%,respectively, and the activity of PL and PM of the five strains rose 150.35%~218.56% and 341.07%~418.52%, respectively. These findings suggested that W. anomalus strains could be used as coffee starter and had great potential for the lysis of pectin.展开更多
文摘病例女,53岁,因"咳嗽、胸部震痛4周,伴痰中带血丝及咳粉红色泡沫痰3周"入院。院外抗炎治疗无效。查体:体温36.9℃,血压116/62 mm Hg(1 mm Hg≈0.133 k Pa),心率为77次/min,心尖区可闻及舒张期杂音,双下肢轻度凹陷性水肿。实验室检查:心肌酶谱正常,D-二聚体1049 ug/L(参考值0~450 ug/L)。胸部CT平扫:两肺小叶间隔轻度增厚(图1);心脏略增大,二尖瓣区见低密度肿块影(图2),CT值约为32 HU;心包以及双侧胸腔内弧形液性低密度影。
文摘In order to improve the pectin-degrading efficiency in wet processing of Arabica coffee in Yunnan, Box-Behnken design and single factor experiment were used to optimize the fermentation conditions of five pectinolytic Wickerhamomyces anomalus strains from the fermentation broth of Arabica coffee in Baoshan, Yunnan during wet processing with pectase activity as an indicator. The results showed that the five strains all synthesized pectin lyase(PL), polygalacturonase(PG), and pectin methylesterase(PM).Among them, strain CAP5 had strong ability to produce PG and PL,while strain CAP4 secreted a large amount of PM. Under optimized conditions, the activity of PG, PL, and PM of the five strains came in at 250.17~411.20 U/mL, 12.98~16.55 U/mL, and 208.52~322.83 U/mL,respectively. The four factors of nitrogen source concentration,fermentation time, Mn2+ concentration, and pH value were optimized and the optimal pectinase-producing fermentation conditions for five strains were as follows: peptone 2.2 g/L, fermentation time 30 h, Mn2+ 1.5 mmol/L, and pH 4.3. After fermentation under the optimized conditions, the maximum PG activity of CAP5 amounted to 411.20 U/mL, 114.03% higher than that before optimization.Meanwhile, the PG activity of strains CAP3, CAP4, CAP8, and CAP10 increased by 86.74%, 114.55%, 65.79%, and 66.07%,respectively, and the activity of PL and PM of the five strains rose 150.35%~218.56% and 341.07%~418.52%, respectively. These findings suggested that W. anomalus strains could be used as coffee starter and had great potential for the lysis of pectin.
