UPLC Q-Exactive Orbitrap MS分析栀子水提液中的化学成分

利用超高效液相色谱-四级杆-静电场轨道阱联用技术(UPLC-Q-Exactive Orbitrap-MS)得到各成分精确的分子量和二级质谱碎片信息,对栀子水提液中的成分进行结构推测和鉴定。采用ACQUITY UPLC BEH C18色谱柱(150 mm×2.1 mm,1.7μm),以乙腈-0.1%甲酸水溶液进行梯度洗脱,流速:0.3 mL/min,柱温:40℃。质谱采用电喷雾离子源(ESI),正负离子监测模式。结果从栀子水提液中检测出了54种化学成分,鉴定了44种化学成分的结构,为进一步研究栀子的药理活性和临床用药奠定物质基础。

单晶氮化硅纳米线的合成制备与光致发光性能研究

本文采用氮气保护高能球磨及硅粉直接氮化工艺制备高纯、单晶氮化硅纳米线。使用X射线衍射仪(XRD)、扫描电子显微镜(SEM)、高分辨率透射电子显微镜(HRTEM)和激光共聚焦显微拉曼光谱仪对实验产物的物相结构、微观结构及光学性能进行分析。在室温下使用325nm激光对样品进行激发,观察到样品具有较宽的发射光谱,波长范围为350~670nm;发光光谱中存在三个由缺陷跃迁引起的发光峰。

深圳市市售茯砖茶氟含量现状探究

目的通过深圳市市售茯砖茶氟含量的现状,探究茯砖茶氟含量超标的成因。方法在深圳市全市范围内采集75份茯砖茶,对每份样品进行氟含量的检测,按照GB19965—2005《砖茶含氟量》判定是否合格。结果所分析的75批次茯砖茶中,50批次氟含量低于GB 19965—2005的限值(300 mg/kg),合格率为66.7%,检测结果平均值为349 mg/kg。结论深圳市市售茯砖茶的合格率高于全国饮茶型地氟病监测点总的砖茶氟含量合格率,茯砖茶氟摄入过量风险较低,但仍存在部分不符合国家强制标准的产品在售,部分茯砖茶的氟含量较高与其原材料的选取有关,建议砖茶的相关产品标准明确氟含量的限量要求。

喀斯特地区非物质文化遗产空间格局及影响因素——以贵州省为例

厘清喀斯特地区非遗分布的空间格局有利于盘点资源现状,客观了解区域文化产生过程,以提高非遗的管理、保护与开发水平。基于地理学空间视角,利用GIS空间分析方法,探讨贵州9个地州市1023项非物质文化遗产类型结构、空间格局及影响因素,研究发现:(1)贵州省非物质文化遗产项目类型结构层次分明,民俗类数量最多,曲艺、传统医药与传统美术类相对稀缺;(2)贵州省非物质文化遗产空间分布不均衡,以贵阳市和黔东南州为双核心,中部和东部集聚明显,西部地区分布较少;(3)贵州省近90%的非物质文化遗产集中分布在海拔1 400 m以下的喀斯特低山、中低山区域,曲艺类占比最高,传统体育、游艺与杂技和传统技艺两类分布与主要河流流域拟合较好,民间文学类与明清时期主要交通干线空间分布关联较高;(4)地形地貌、水系、文化、交通、经济等自然和人文因素对喀斯特地区非物质文化遗产空间格局产生重要影响。

Clinical application and analysis of hepatitis C virus NS3 antigen detection by ELISA in human serum

Background Hepatitis C virus （HCV） core antigen assays have been produced to exclude infectious donations collected during the preseroconversion window phase （PWP）. For the same purpose, we evaluated the specificity and sensitivity of a novel hepatitis C virus NS3 antigen detection immunoassay and the application of this assay in clinical diagnosis. Methods Samples from 77 healthy subjects, 173 anti-HCV positive patients and 3708 hepatitis patients other than HCV positive were tested with the HCV NS3 antigen assay. Some HCV NS3 antigen positive samples were further validated with HCV-RNA, neutralization and immunodot assays. Twenty-five sequential samples from 11 HCV NS3 antigen positive patients were subjected to kinetic study. Results Only 48 （1.3%） of 3708 anti-HCV negative samples were positive for HCV NS3 antigen. Among them, 44 of 3030 samples from patients only infected with HBV were HCV NS3 antigen positive, 4 of the 445 samples from patients infected with other type hepatitis were HCV NS3 antigen positive. In addition, 42 （24.3%） of 173 anti-HCV positive samples were HCV NS3 antigen positive and all 77 samples from healthy subjects were negative to HCV NS3 antigen assay. Of the 15 HCV NS3 antigen positive samples, 9 （60%） were HCV-RNA positive. The neutralization and positive percentage of immunodot assay for 23 HCV NS3 antigen positive sera were 87.0% （20/23） and 69.6% （16/23） respectively. Of the 25 sequential samples from 11 HCV NS3 antigen positive patients, there was a negative correlation between the OD values and the duration of test （r=-0.989, P〈0.05）, and there were correlations among their HCV NS3 antigen, HCV-RNA and anti-HCV Utres. The anti-HCV antibodies of two sera were detected while their OD values of HCV NS3 antigen decreased gradually. Conclusions The HCV NS3 antigen detection assay showed perfect specificity and high sensitivity. Thus, it would be useful and economical as a routine test in laboratories for early diagnosis of HCV infection and prevention.