In the present study, a full-length cDNA encoding 1-aminocyclopropane-1-carboxylic acid oxidase gene has been cloned from sugarcane （named GZ-ACO）. Two primers were designed for coding the ORF in the full-length cDN...In the present study, a full-length cDNA encoding 1-aminocyclopropane-1-carboxylic acid oxidase gene has been cloned from sugarcane （named GZ-ACO）. Two primers were designed for coding the ORF in the full-length cDNA of GZ-ACO gene from sugarcane. PCR amplification was performed with sugarcane DNA template, and a fragment of 1 104 bp （GZ34） was obtained. GZ34 was labeled with [α-32P] dCTP as the probe and used for hybridization after cloning and sequencing. Southern blotting analysis indicated that there were at least three other sequences, which weakly hybridized with the GZ34. Northern analysis showed that GZ34 was strongly induced by treatment with IAA, BA, ethephon, LiC1 and cold stress, respectively. As a contrast, the mRNA for ACO gene was at lower levels for both the light-grown and dark-grown plants without additional treatment. There were two transcripts in the dark-grown plants and three transcripts in the treatments with IAA, BA and cold stress, but there was only one transcript in ethephon treatment. It showed that GZ-ACO might be a gene connected with ethylene formation and take part in response to the induction of plant hormone and environmental stress.展开更多
Resistance to rice gall midge in rice germplasm 91-1A2 was identified and genetically analyzed F1s of rice population were derived from 91-1A2 which crossed with rice materials Jinggui, TN1, W1263 （Gm1）, IET2911 （...Resistance to rice gall midge in rice germplasm 91-1A2 was identified and genetically analyzed F1s of rice population were derived from 91-1A2 which crossed with rice materials Jinggui, TN1, W1263 （Gm1）, IET2911 （Gm2）, BG404-1 （gm3）, OB677 （Gm4）, ARC5984 （Gm5） and Duokang 1 （Gm6） as a male parent. The resistance of all parental lines and F1, BC1F1 and F2 populations to rice gall midge was identified. The results showed that 91-1A2 and all F1s were resistant to Chinese rice gall midge biotype IV. The segregation ratio of resistant plants to susceptible ones in BC1F1 and F2 were accorded with 1：3 and 9：7 rules by X2 test, suggesting that the resistance of 91-1A2 to Chinese rice gall midge biotype IV was controlled by two dominant genes which were new resistance genes, non-allelic to the known rice gall midge resistance genes.展开更多
基金the National Natural Science Foundation of China(39860039).
文摘In the present study, a full-length cDNA encoding 1-aminocyclopropane-1-carboxylic acid oxidase gene has been cloned from sugarcane （named GZ-ACO）. Two primers were designed for coding the ORF in the full-length cDNA of GZ-ACO gene from sugarcane. PCR amplification was performed with sugarcane DNA template, and a fragment of 1 104 bp （GZ34） was obtained. GZ34 was labeled with [α-32P] dCTP as the probe and used for hybridization after cloning and sequencing. Southern blotting analysis indicated that there were at least three other sequences, which weakly hybridized with the GZ34. Northern analysis showed that GZ34 was strongly induced by treatment with IAA, BA, ethephon, LiC1 and cold stress, respectively. As a contrast, the mRNA for ACO gene was at lower levels for both the light-grown and dark-grown plants without additional treatment. There were two transcripts in the dark-grown plants and three transcripts in the treatments with IAA, BA and cold stress, but there was only one transcript in ethephon treatment. It showed that GZ-ACO might be a gene connected with ethylene formation and take part in response to the induction of plant hormone and environmental stress.
基金supported by the Natural Science Foundation of Guangxi Province (Grant No.0007015)Science Research and Technology Development Program of Guangxi Province (Grant Nos.0012027 and 9939006)Foundation of New Century Ten-Hundred-Thous and Talents of Guangxi,China (Grant No. 2003213)
文摘Resistance to rice gall midge in rice germplasm 91-1A2 was identified and genetically analyzed F1s of rice population were derived from 91-1A2 which crossed with rice materials Jinggui, TN1, W1263 （Gm1）, IET2911 （Gm2）, BG404-1 （gm3）, OB677 （Gm4）, ARC5984 （Gm5） and Duokang 1 （Gm6） as a male parent. The resistance of all parental lines and F1, BC1F1 and F2 populations to rice gall midge was identified. The results showed that 91-1A2 and all F1s were resistant to Chinese rice gall midge biotype IV. The segregation ratio of resistant plants to susceptible ones in BC1F1 and F2 were accorded with 1：3 and 9：7 rules by X2 test, suggesting that the resistance of 91-1A2 to Chinese rice gall midge biotype IV was controlled by two dominant genes which were new resistance genes, non-allelic to the known rice gall midge resistance genes.
