Objective:To explore the related mechanism of miR-34a affecting epilepsy.Methods:MicroRNA microarray chip and RT-PCR were used to analyze the expression of miR-34a in epileptic rats,and observe the relationship betwee...Objective:To explore the related mechanism of miR-34a affecting epilepsy.Methods:MicroRNA microarray chip and RT-PCR were used to analyze the expression of miR-34a in epileptic rats,and observe the relationship between miR-34a and seizure frequency and EEG.Predicted the target gene of miR-34a,used The dual-luciferase reporter gene assay was used to observe its binding to the target gene of miR-34a,the effect of overexpression and inhibition of miR-34a on the target gene,the effects of overexpression and inhibition of target genes on downstream factors.Results:Microarray chip and RT-PCR detection showed that miR-34a was highly expressed in epilepsy(P<0.05),and miR-34a was positively correlated with seizure frequency(R=0.783,P=0.003),and inhibition of miR-34a inhibited the seizure frequency of epilepsy(P<0.05).The online databases TargetScan and miRDB show that miR-34a and BCL-2 have a predicted binding region,and the dual-luciferase reporter gene experiment proves that the two have a binding relationship.When miR-34a was overexpressed,BCL-2 expression was down-regulated,and conversely,when miR-34a was inhibited,BCL-2 expression was up-regulated.NLRP1 and downstream related factors Caspase-3,Caspase-1,IL-1βand IL-18 were highly expressed in the brain tissue of epileptic rats(P<0.05).When BCL-2 was inhibited,the expression levels of NLRP1 and downstream related factors were increased,and when BCL-2 was inhibited,the result was the opposite.Conclusion:The highly expressed miR-34a promotes epilepsy by inhibiting BCL-2 and activating the NLRP1 inflammasome.展开更多
To review the research progress on the mechanism of traditional Chinese medicine(TCM)treatment of depression based on NLRP3 pathway in the past five years,in order to provide the basis for the clinical application of ...To review the research progress on the mechanism of traditional Chinese medicine(TCM)treatment of depression based on NLRP3 pathway in the past five years,in order to provide the basis for the clinical application of TCM treatment of depression.NLRP3,TCM and depression were used as key words to search PubMed and China Journal Fulltext Database(CNKI)and other databases for the relevant experimental literatures on antidepressant effects of TCM based on NLRP3 pathway in recent years.Chinese medicine can protect nerve cells by inhibiting the activation of NLRP3 and reduce the depression-like behavior in animal model.TCM can inhibit NLRP3 pathway and exert anti-depressive effect.展开更多
基金Guangxi Natural Science Foundation(No.2020GXNSFAA238007)Guangxi High-level Innovation Team and Outstanding Scholars Program[Guangxi Higher Education Talents(2020)No.6]+2 种基金Academic Team Building Project of the First Affiliated Hospital of Guangxi TCM University(NO:Courtyard Word[2018]No.146)Guangxi Clinical Research Center of Traditional Chinese Medicine encephalopathy(NO.AD20238028)Guangxi Traditional Chinese Medicine Key Discipline Construction Project(No.GZXK-Z-20-13)。
文摘Objective:To explore the related mechanism of miR-34a affecting epilepsy.Methods:MicroRNA microarray chip and RT-PCR were used to analyze the expression of miR-34a in epileptic rats,and observe the relationship between miR-34a and seizure frequency and EEG.Predicted the target gene of miR-34a,used The dual-luciferase reporter gene assay was used to observe its binding to the target gene of miR-34a,the effect of overexpression and inhibition of miR-34a on the target gene,the effects of overexpression and inhibition of target genes on downstream factors.Results:Microarray chip and RT-PCR detection showed that miR-34a was highly expressed in epilepsy(P<0.05),and miR-34a was positively correlated with seizure frequency(R=0.783,P=0.003),and inhibition of miR-34a inhibited the seizure frequency of epilepsy(P<0.05).The online databases TargetScan and miRDB show that miR-34a and BCL-2 have a predicted binding region,and the dual-luciferase reporter gene experiment proves that the two have a binding relationship.When miR-34a was overexpressed,BCL-2 expression was down-regulated,and conversely,when miR-34a was inhibited,BCL-2 expression was up-regulated.NLRP1 and downstream related factors Caspase-3,Caspase-1,IL-1βand IL-18 were highly expressed in the brain tissue of epileptic rats(P<0.05).When BCL-2 was inhibited,the expression levels of NLRP1 and downstream related factors were increased,and when BCL-2 was inhibited,the result was the opposite.Conclusion:The highly expressed miR-34a promotes epilepsy by inhibiting BCL-2 and activating the NLRP1 inflammasome.
基金Guangxi Natural Science Foundation Project(No.2020JJA140287)2020 Guangxi Higher Education High-level Innovation Team and Outstanding Scholars Program[(2020)No.6]+1 种基金Guangxi University of Traditional Chinese Medicine"Qihuang Project"High-level Talent Team Cultivation Project(No.2018003)Academic Team Construction Project of the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine[(2018)No.146]。
文摘To review the research progress on the mechanism of traditional Chinese medicine(TCM)treatment of depression based on NLRP3 pathway in the past five years,in order to provide the basis for the clinical application of TCM treatment of depression.NLRP3,TCM and depression were used as key words to search PubMed and China Journal Fulltext Database(CNKI)and other databases for the relevant experimental literatures on antidepressant effects of TCM based on NLRP3 pathway in recent years.Chinese medicine can protect nerve cells by inhibiting the activation of NLRP3 and reduce the depression-like behavior in animal model.TCM can inhibit NLRP3 pathway and exert anti-depressive effect.
