To alleviate the chattering problem, a new type of fuzzy global sliding mode controller （FGSMC） is presented. In this controller, the switching gain is estimated by fuzzy logic system based on the reachable conditio...To alleviate the chattering problem, a new type of fuzzy global sliding mode controller （FGSMC） is presented. In this controller, the switching gain is estimated by fuzzy logic system based on the reachable conditions of sliding mode controller（SMC）, and genetic algorithm （GA） is used to optimize scaling factor of the switching gain, thus the switch chattering of SMC can be alleviated. Moreover, global sliding mode is realized by designing an exponential dynamic sliding surface. Simulation and real-time application for flight simulator servo system with Lugre friction are given to indicate that the proposed controller can guarantee high robust performance all the time and can alleviate chattering phenomenon effectively.展开更多
To improve the robustness of high-precision servo systems, quantitative feedback theory （QFT） which aims to achieve a desired robust design over a specified region of plant uncertainty is proposed. The robust design...To improve the robustness of high-precision servo systems, quantitative feedback theory （QFT） which aims to achieve a desired robust design over a specified region of plant uncertainty is proposed. The robust design problem can be solved using QFT but it fails to guarantee a high precision tracking. This problem is solved by a robust digital QFT control scheme based on zero phase error （ZPE） feed forward compensation. This scheme consists of two parts： a QFT controller in the closed-loop system and a ZPE feed-forward compensator. Digital QFT controller is designed to overcome the uncertainties in the system. Digital ZPE feed forward controller is used to improve the tracking precision. Simulation and real-time examples for flight simulator servo system indicate that this control scheme can guarantee both high robust performance and high position tracking precision.展开更多
目的:探索共表达双特异性T细胞接合器(bispecific T cell engager,BiTE)的VSVΔ51溶瘤病毒对骨肉瘤的抗肿瘤活性。方法:将BiTE的全长序列克隆到病毒糖蛋白和聚合酶基因之间产生VSVΔ51/BiTE溶瘤病毒;使用小鼠异源移植瘤模型评估VSVΔ51/...目的:探索共表达双特异性T细胞接合器(bispecific T cell engager,BiTE)的VSVΔ51溶瘤病毒对骨肉瘤的抗肿瘤活性。方法:将BiTE的全长序列克隆到病毒糖蛋白和聚合酶基因之间产生VSVΔ51/BiTE溶瘤病毒;使用小鼠异源移植瘤模型评估VSVΔ51/BiTE的肿瘤抑制活性;通过免疫组化检测肿瘤组织的T细胞浸润情况;通过流式细胞术检测肿瘤浸润T细胞的激活状态。结果:成功构建VSVΔ51/BiTE溶瘤病毒,并通过Western blot验证其可以表达BiTE;体内抑瘤实验结果显示,VSVΔ51/BiTE较VSVΔ51的肿瘤抑制能力更强(P<0.0001);免疫组化结果表明,VSVΔ51/BiTE治疗后肿瘤浸润T细胞的数量较VSVΔ51治疗组显著增多(P<0.0001);流式检测结果表明,VSVΔ51/BiTE治疗可以有效增强肿瘤浸润T细胞的增殖能力(P<0.001),并提高了CD107a的表达水平以及干扰素-γ(interferon-γ,IFN-γ)的分泌水平(P<0.0001)。结论:溶瘤病毒VSVΔ51/BiTE可以显著抑制小鼠骨肉瘤的生长,同时激活肿瘤部位的抗肿瘤免疫反应,是一种非常有希望的骨肉瘤治疗策略。展开更多
基金This project is supported by Aeronautics Foundation of China (No. 00E51022)
文摘To alleviate the chattering problem, a new type of fuzzy global sliding mode controller （FGSMC） is presented. In this controller, the switching gain is estimated by fuzzy logic system based on the reachable conditions of sliding mode controller（SMC）, and genetic algorithm （GA） is used to optimize scaling factor of the switching gain, thus the switch chattering of SMC can be alleviated. Moreover, global sliding mode is realized by designing an exponential dynamic sliding surface. Simulation and real-time application for flight simulator servo system with Lugre friction are given to indicate that the proposed controller can guarantee high robust performance all the time and can alleviate chattering phenomenon effectively.
基金This project was supported by the Aeronautics Foundation of China (00E51022).
文摘To improve the robustness of high-precision servo systems, quantitative feedback theory （QFT） which aims to achieve a desired robust design over a specified region of plant uncertainty is proposed. The robust design problem can be solved using QFT but it fails to guarantee a high precision tracking. This problem is solved by a robust digital QFT control scheme based on zero phase error （ZPE） feed forward compensation. This scheme consists of two parts： a QFT controller in the closed-loop system and a ZPE feed-forward compensator. Digital QFT controller is designed to overcome the uncertainties in the system. Digital ZPE feed forward controller is used to improve the tracking precision. Simulation and real-time examples for flight simulator servo system indicate that this control scheme can guarantee both high robust performance and high position tracking precision.
文摘目的:探索共表达双特异性T细胞接合器(bispecific T cell engager,BiTE)的VSVΔ51溶瘤病毒对骨肉瘤的抗肿瘤活性。方法:将BiTE的全长序列克隆到病毒糖蛋白和聚合酶基因之间产生VSVΔ51/BiTE溶瘤病毒;使用小鼠异源移植瘤模型评估VSVΔ51/BiTE的肿瘤抑制活性;通过免疫组化检测肿瘤组织的T细胞浸润情况;通过流式细胞术检测肿瘤浸润T细胞的激活状态。结果:成功构建VSVΔ51/BiTE溶瘤病毒,并通过Western blot验证其可以表达BiTE;体内抑瘤实验结果显示,VSVΔ51/BiTE较VSVΔ51的肿瘤抑制能力更强(P<0.0001);免疫组化结果表明,VSVΔ51/BiTE治疗后肿瘤浸润T细胞的数量较VSVΔ51治疗组显著增多(P<0.0001);流式检测结果表明,VSVΔ51/BiTE治疗可以有效增强肿瘤浸润T细胞的增殖能力(P<0.001),并提高了CD107a的表达水平以及干扰素-γ(interferon-γ,IFN-γ)的分泌水平(P<0.0001)。结论:溶瘤病毒VSVΔ51/BiTE可以显著抑制小鼠骨肉瘤的生长,同时激活肿瘤部位的抗肿瘤免疫反应,是一种非常有希望的骨肉瘤治疗策略。
