为探究湖北襄阳地区高温大曲中芽孢杆菌(Bacillus)菌群多样性,该研究通过下载并筛选高温大曲样品芽孢杆菌相对含量>2.0%的测序数据,采用Illumina Mi Seq高通量测序技术和传统纯培养技术相结合的方法对大曲样品中芽孢杆菌多样性进行...为探究湖北襄阳地区高温大曲中芽孢杆菌(Bacillus)菌群多样性,该研究通过下载并筛选高温大曲样品芽孢杆菌相对含量>2.0%的测序数据,采用Illumina Mi Seq高通量测序技术和传统纯培养技术相结合的方法对大曲样品中芽孢杆菌多样性进行解析。结果表明,高温大曲中芽孢杆菌相对含量>2.0%的样品共有12份,包括8份白色大曲样品和4份黄色大曲样品,所有样品共获得77 825条代表性序列,1 197个操作分类单元(OTUs)。多样性分析结果表明,较之黄色大曲,白色大曲样品中芽孢杆菌的超1指数和发现物种数显著偏高(P<0.05)。OTU分析结果表明,OTU5321和OTU5291在所有样品中均存在,其在白色大曲和黄色大曲中累积包含序列分别占总序列数的49.43%和41.32%。传统纯培养技术分析结果表明,解淀粉芽孢杆菌(Bacillus amyloliquefaciens)为高温大曲中芽孢杆菌的主要可培养菌种。由此可知,白色大曲芽孢杆菌群落结构的丰富度更高,且解淀粉芽孢杆菌为高温大曲中芽孢杆菌的主要可培养菌种,为后续高温大曲中芽孢杆菌的应用及菌种选育具有指导作用。展开更多
为探究湖北襄阳地区不同颜色高温大曲中葡萄球菌(Staphylococcus)多样性,该研究通过下载并筛选葡萄球菌属相对含量>10%的样品序列,采用Illumina Mi Seq高通量测序技术和传统纯培养技术相结合的方法对大曲中葡萄球菌多样性进行解析。...为探究湖北襄阳地区不同颜色高温大曲中葡萄球菌(Staphylococcus)多样性,该研究通过下载并筛选葡萄球菌属相对含量>10%的样品序列,采用Illumina Mi Seq高通量测序技术和传统纯培养技术相结合的方法对大曲中葡萄球菌多样性进行解析。结果表明,高温大曲中葡萄球菌相对含量>10%的样品共有13份,包括7份黑色大曲、4份黄色大曲和2份白色大曲,葡萄球菌基因序列相对丰度占所有原核生物的14.33%~74.19%。多样性分析表明,黑色大曲中葡萄球菌丰富度和多样性均显著偏高(P<0.05),且不同颜色高温大曲中葡萄球菌群落结构差异显著(P<0.05)。操作分类单元(OTU)分析表明,高温大曲中共有OTU 5个,累积平均相对含量达到91.38%。传统纯培养技术表明,腐生葡萄球菌(Staphylococcus saprophyticus)是黑色、黄色和白色大曲中共有的可培养菌种,亦是高温大曲中葡萄球菌的主要可培养菌种。由此表明,黑色大曲中葡萄球菌丰富度和多样性更高,且腐生葡萄球菌为高温大曲中葡萄球菌的主要可培养菌种。展开更多
The hepatopancreatic necrosis disease(HPND)is a common shrimp disease.Aquaculture of the black tiger shrimp Penaeus monodon has suffered with the frequent outbreak of HPND.Understanding the molecular mechanisms of hos...The hepatopancreatic necrosis disease(HPND)is a common shrimp disease.Aquaculture of the black tiger shrimp Penaeus monodon has suffered with the frequent outbreak of HPND.Understanding the molecular mechanisms of host response to HPND is of pivotal importance for improving its farming performance.In this study,the RNA-Seq platform was utilized to investigate transcriptomic changes of the hepatopancreas in pond-cultured P.monodon with HPND symptoms.A total of 62071 genes with an average length of 953 bp were obtained,and a lot of simple sequence repeat(SSR)loci related to these genes were identified.Totally,5204 differentially expressed genes(DEGs)were detected between healthy shrimp and HPND shrimp,with 3399 genes upregulated and 1,805 genes down-regulated.These genes had a wide range of biological functions,and several well-known immunerelated genes including integrin alpha 5,integrin beta 1,C type lectin and catalase were among the DEGs.Cell signaling pathways including the extracellular matrix(ECM)receptor interaction,phagosome and lysosome pathways were significantly upregulated in HPND-infected shrimps,indicating their involvement in the immune responses of shrimp against HPND.The data obtained in this study offers new insights into the molecular mechanisms of shrimp host response to HPND disease,and provides a resource for molecular marker development and genetic breeding studies of P.monodon.展开更多
该研究采用Illumina Mi Seq高通量测序技术对3份人工窖泥样品的微生物菌群多样性进行解析,并对优势菌属间的相关性进行分析。结果表明,人工窖泥中微生物组成丰富,优势细菌属(平均相对丰度>1%)为魏斯氏菌属(Weissella)、真杆菌属(Euba...该研究采用Illumina Mi Seq高通量测序技术对3份人工窖泥样品的微生物菌群多样性进行解析,并对优势菌属间的相关性进行分析。结果表明,人工窖泥中微生物组成丰富,优势细菌属(平均相对丰度>1%)为魏斯氏菌属(Weissella)、真杆菌属(Eubacterium)、新斯氏菌属(Neoscardovia)、片球菌属(Pediococcus)、芽孢杆菌属(Bacillus)、Caproicibacter、高温放线菌属(Thermoactinomyces)、棒状杆菌属(Corynebacterium)、真丝菌属(Planifilum)、狭义梭菌属(Clostridium sensu stricto)、肠球菌属(Enterococcus)、链霉菌属(Streptomyces)和联合乳杆菌属(Ligilactobacillus);优势真菌属(平均相对丰度>1%)为耐干霉菌属(Xeromyces)、曲霉属(Aspergillus)、节担菌属(Wallemia)、Trichomonascus、根霉属(Rhizopus)和毕赤酵母属(Pichia)。其中,Planifilum与Bacillus和Ligilactobacillus与Enterococcus的协同效应对人工窖泥品质有正向调节作用,而Aspergillus与Enterococcus、Thermoactinomyces及Pichia与Weissella之间存在显著的拮抗或竞争关系(P<0.05)。展开更多
文摘为探究湖北襄阳地区不同颜色高温大曲中葡萄球菌(Staphylococcus)多样性,该研究通过下载并筛选葡萄球菌属相对含量>10%的样品序列,采用Illumina Mi Seq高通量测序技术和传统纯培养技术相结合的方法对大曲中葡萄球菌多样性进行解析。结果表明,高温大曲中葡萄球菌相对含量>10%的样品共有13份,包括7份黑色大曲、4份黄色大曲和2份白色大曲,葡萄球菌基因序列相对丰度占所有原核生物的14.33%~74.19%。多样性分析表明,黑色大曲中葡萄球菌丰富度和多样性均显著偏高(P<0.05),且不同颜色高温大曲中葡萄球菌群落结构差异显著(P<0.05)。操作分类单元(OTU)分析表明,高温大曲中共有OTU 5个,累积平均相对含量达到91.38%。传统纯培养技术表明,腐生葡萄球菌(Staphylococcus saprophyticus)是黑色、黄色和白色大曲中共有的可培养菌种,亦是高温大曲中葡萄球菌的主要可培养菌种。由此表明,黑色大曲中葡萄球菌丰富度和多样性更高,且腐生葡萄球菌为高温大曲中葡萄球菌的主要可培养菌种。
基金This study was funded in part by the Natural Science Foundation of Guangdong Province(No.2018A030310049)2019 Annual Guangdong Provincial Special fund of Nan-hai Economic Shrimp Breeding and Culture Laboratory(No.2319412525)+2 种基金the Nanhai Scholar Project of Guangdong Ocean University,Fangchenggang Science and Technol-ogy Plan Project(No.AD19008017)the R&D Program of Key Areas in Guangdong Province(No.2020B0202010009)2019 Provincial Financial Special Fund Con-struction Project of Guangdong Ocean University(No.231619003).
文摘The hepatopancreatic necrosis disease(HPND)is a common shrimp disease.Aquaculture of the black tiger shrimp Penaeus monodon has suffered with the frequent outbreak of HPND.Understanding the molecular mechanisms of host response to HPND is of pivotal importance for improving its farming performance.In this study,the RNA-Seq platform was utilized to investigate transcriptomic changes of the hepatopancreas in pond-cultured P.monodon with HPND symptoms.A total of 62071 genes with an average length of 953 bp were obtained,and a lot of simple sequence repeat(SSR)loci related to these genes were identified.Totally,5204 differentially expressed genes(DEGs)were detected between healthy shrimp and HPND shrimp,with 3399 genes upregulated and 1,805 genes down-regulated.These genes had a wide range of biological functions,and several well-known immunerelated genes including integrin alpha 5,integrin beta 1,C type lectin and catalase were among the DEGs.Cell signaling pathways including the extracellular matrix(ECM)receptor interaction,phagosome and lysosome pathways were significantly upregulated in HPND-infected shrimps,indicating their involvement in the immune responses of shrimp against HPND.The data obtained in this study offers new insights into the molecular mechanisms of shrimp host response to HPND disease,and provides a resource for molecular marker development and genetic breeding studies of P.monodon.
文摘该研究采用Illumina Mi Seq高通量测序技术对3份人工窖泥样品的微生物菌群多样性进行解析,并对优势菌属间的相关性进行分析。结果表明,人工窖泥中微生物组成丰富,优势细菌属(平均相对丰度>1%)为魏斯氏菌属(Weissella)、真杆菌属(Eubacterium)、新斯氏菌属(Neoscardovia)、片球菌属(Pediococcus)、芽孢杆菌属(Bacillus)、Caproicibacter、高温放线菌属(Thermoactinomyces)、棒状杆菌属(Corynebacterium)、真丝菌属(Planifilum)、狭义梭菌属(Clostridium sensu stricto)、肠球菌属(Enterococcus)、链霉菌属(Streptomyces)和联合乳杆菌属(Ligilactobacillus);优势真菌属(平均相对丰度>1%)为耐干霉菌属(Xeromyces)、曲霉属(Aspergillus)、节担菌属(Wallemia)、Trichomonascus、根霉属(Rhizopus)和毕赤酵母属(Pichia)。其中,Planifilum与Bacillus和Ligilactobacillus与Enterococcus的协同效应对人工窖泥品质有正向调节作用,而Aspergillus与Enterococcus、Thermoactinomyces及Pichia与Weissella之间存在显著的拮抗或竞争关系(P<0.05)。
