Five Rutaceae family ethanol extracts alleviate H2O2 and LPS-induced inflammation via NF-κB and JAK-STAT3 pathway in HaCaT cells

This study was designed to investigate the effects of five Rutaceae family ethanol extracts(FRFEE):Citrus medica Linn(CML),Citrus aurantium L.Cv.Daidai(CAD),Citrus medica Linn.var.sarcodactylis(Noot.)Swingle(CMS),Citrus sinensis L.Osbeck(CSO)and Zanthoxylum bungeanum Maxim(ZBM)on retarding the progression of H_(2)O_(2) and LPS-induced HaCaT cells.Cell inflammatory injury model was established by H_(2)O_(2) and LPS.The alleviative effects of FRFEE were evaluated by detecting the activity of superoxide dismutase(SOD),glutathione(GSH)and the generation of reactive oxygen species(ROS).The inflammatory signaling pathways of NF-κB and JAK-STAT3 were detected by Western blotting,the mRNA expression levels of inflammatory factors and skin barrier factors were detected by RT-PCR.50%ethanol extracts of five medicinal and food homologous herbs of Rutaceae family showed different levels of anti-oxidant and anti-inflammatory activities.The FRFEE effectively improved SOD and GSH content and decreased ROS levels.Meanwhile,FRFEE strongly suppressed two inflammatory signaling pathways NF-κB and JAK-STAT3.The RT-PCR examination of inflammatory factors and skin barrier factor revealed significant anti-inflammatory effects of FRFEE.It was worth noting that among the five extracts,Zanthoxylum bungeanum Maxim extract had the best anti-inflammatory and anti-oxidation effects.In addition,it could strongly inhibit the expression of psoriasis factor CCL20.In summary,these results suggested that Zanthoxylum bungeanum Maxim extract could be used as an anti-psoriatic agent in the treatment of psoriasis among FRFEE.