The polysaccharides extracted from Inula britannica flower（IBP） exhibited a significant anti-liver injury activity.The properties and chemical compositions of IBP were analyzed via high performance liquid chromatogr...The polysaccharides extracted from Inula britannica flower（IBP） exhibited a significant anti-liver injury activity.The properties and chemical compositions of IBP were analyzed via high performance liquid chromatography（HPLC） and infrared spectroscopy（IR） methods.The results show that the molecular weight distribution of IBP is from 600 to 3500.The weight-average molecular weight is 1099.IBP consists of mannose,glucuronic acid,rhamnose,galacturonic acid,glucose,galactose and arabinose with a molar ratio of 4.1：1：1.4：2.7：14.6：6.3：7.9.The IR spectrum of IBP reveals the typical characteristics of polysaccharides and proteins.After administration of IBP with 100,50 and 25 mg/kg body mass to liver injury mice induced by CCl 4 or bacillus calmette guerin-lipopolysaccharide（BCG-LPS）,not only the blood alanine aminotransferase（ALT） and aspartate aminotransferase（AST） levels decreased significantly（P0.05）,but aslo the liver tissue malondialdehyde（MDA） level decreased significantly（P0.01）.The superoxide dismutase（SOD） level increased significantly（P0.01）.IBP shows significant anti-liver injury and antioxidation activity.展开更多
Aiming at isolating and investigating the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory activity, the authors characterized one ingredient. To separate the oligosaecharides...Aiming at isolating and investigating the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory activity, the authors characterized one ingredient. To separate the oligosaecharides and polypeptides, a DEAE-Sephadex A-50 colum was used. The enhanced memory activity in mice was studied by Mirros water maze tesk in mice. The dose of oligosacchrides, polypeptides or Piracetam was 30 mg/kg per day with intraperitoneal administration. The oligosaccharides did not show enhancing memory effect, but polypeptides did show. This result demonstrates that the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory effect was polypeptides. The purification of the polypeptides was performed on a Sephadex G-25 column. A novel tetradeeapeptide was purified from the polypeptides and its structure was determined by liquid chromatography-eleetrospray ionization-quadrupole-time of flight-mass spectrometry（LC-ESI-Q-TOF-MS） with the amino acid sequence of Lys-Ser-Leu-Thr-Leu-Thr-Ser-Ser-Leu-Ser-Tyr-Thr-Asp-Ser.展开更多
基金Supported by the Science and Technology Development Program of Jilin Province,China(No.20070917-01)the Technology Development Project of Jilin Province,China(No.201205020)
文摘The polysaccharides extracted from Inula britannica flower（IBP） exhibited a significant anti-liver injury activity.The properties and chemical compositions of IBP were analyzed via high performance liquid chromatography（HPLC） and infrared spectroscopy（IR） methods.The results show that the molecular weight distribution of IBP is from 600 to 3500.The weight-average molecular weight is 1099.IBP consists of mannose,glucuronic acid,rhamnose,galacturonic acid,glucose,galactose and arabinose with a molar ratio of 4.1：1：1.4：2.7：14.6：6.3：7.9.The IR spectrum of IBP reveals the typical characteristics of polysaccharides and proteins.After administration of IBP with 100,50 and 25 mg/kg body mass to liver injury mice induced by CCl 4 or bacillus calmette guerin-lipopolysaccharide（BCG-LPS）,not only the blood alanine aminotransferase（ALT） and aspartate aminotransferase（AST） levels decreased significantly（P0.05）,but aslo the liver tissue malondialdehyde（MDA） level decreased significantly（P0.01）.The superoxide dismutase（SOD） level increased significantly（P0.01）.IBP shows significant anti-liver injury and antioxidation activity.
基金Supported by the National Natural Science Foundation of China（No.81173532）, the Science and Technology Development Project of Jilin Province, China（No.201201086）, the Traditional Chinese Medicine Science and Technology Project from Jilin Provincial Administration of Traditional Chinese Medicine, China（No.2012-114）.
文摘Aiming at isolating and investigating the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory activity, the authors characterized one ingredient. To separate the oligosaecharides and polypeptides, a DEAE-Sephadex A-50 colum was used. The enhanced memory activity in mice was studied by Mirros water maze tesk in mice. The dose of oligosacchrides, polypeptides or Piracetam was 30 mg/kg per day with intraperitoneal administration. The oligosaccharides did not show enhancing memory effect, but polypeptides did show. This result demonstrates that the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory effect was polypeptides. The purification of the polypeptides was performed on a Sephadex G-25 column. A novel tetradeeapeptide was purified from the polypeptides and its structure was determined by liquid chromatography-eleetrospray ionization-quadrupole-time of flight-mass spectrometry（LC-ESI-Q-TOF-MS） with the amino acid sequence of Lys-Ser-Leu-Thr-Leu-Thr-Ser-Ser-Leu-Ser-Tyr-Thr-Asp-Ser.
