Introduction:The difficulty in treating lung adenocarcinoma(LUAD)is caused by a shortage of knowledge about the biological mechanisms and a lack of treatment choices.Objectives:The aim of this study was to identify a ...Introduction:The difficulty in treating lung adenocarcinoma(LUAD)is caused by a shortage of knowledge about the biological mechanisms and a lack of treatment choices.Objectives:The aim of this study was to identify a valuable molecular target for the treatment of LUAD.Methods:Using multiple databases,we screened for hub genes in LUAD using Cytoscape and explored the expression and prognosis of DLG associated protein 5(DLGAP5)in LUAD.We investigated the genetic variation,functional enrichment,and epigenetic activity of DLGAP5.Furthermore,we evaluated the relationship between the tumor microenvironment(TME)and DLGAP5.Results:Our study identified 10 hub genes in LUAD:CDC45,KIAA0101,DLGAP5,CDT1,NCAPG,CCNB1,CDCA5,CDC20,KIF11,and AURKA.We discovered that DLGAP5 was overexpressed and associated with poor prognosis in LUAD.DLGAP5 exhibited an overall genetic variation frequency of 2%,and its DNA promoter was hypomethylated in LUAD(p<0.05).The expression of DLGAP5 in LUAD showed a positive correlation with the majority of N6-methyladenosine(m6A)-methylation genes.Additionally,DLGAP5 was primarily associated with the cell cycle in LUAD.Notably,there was a significant favorable association between DLGAP5 and CD274,CTLA4,HAVCR2,and LAG3 in LUAD.Conclusion:DLGAP5 may be a therapeutic target for LUAD,as it affects cancer cells proliferation and development through the regulation of cell-cycle checkpoints and modulation of immune cell infiltration and immune checkpoints in the TME.展开更多
Substernal goiter is considered to be a dia-gnostic differential for all anterosuperior mediastinal masses.For a substernal goiter,surgical removal is sug-gested when it is large in size,with a possibility for malig-n...Substernal goiter is considered to be a dia-gnostic differential for all anterosuperior mediastinal masses.For a substernal goiter,surgical removal is sug-gested when it is large in size,with a possibility for malig-nancy or has local compression of adjacent structures.This can be performed through the neck or by the addition of a partial or complete sternotomy if necessary.A 58-year-old Chinese man from Guangdong Province had had dry cough for a year.A subsequent CT scan in our hospital suggested‘‘ectopic intrathoracic thyroid’’.Fine needle percutaneous mass biopsy also suggested‘‘ectopic intrathoracic thyroid’’.We performed a standard median sternotomy to remove the mass.It measured 13 cm 68 cm 66 cm in size,and weighed 2.8 kg.The patho-logical diagnosis confirmed a benign thyroid adenoma.The patient had no hoarseness,dyspnea or hypocalcemia and was quickly extubated in the operating room.Recently,we encountered a big substernal goiter and had performed successful resection,which is reported here for reference.展开更多
基金funded by the supporting funds for scientific research of the Sixth Affiliated Hospital,Sun Yat-sen University(P20200217202404781).
文摘Introduction:The difficulty in treating lung adenocarcinoma(LUAD)is caused by a shortage of knowledge about the biological mechanisms and a lack of treatment choices.Objectives:The aim of this study was to identify a valuable molecular target for the treatment of LUAD.Methods:Using multiple databases,we screened for hub genes in LUAD using Cytoscape and explored the expression and prognosis of DLG associated protein 5(DLGAP5)in LUAD.We investigated the genetic variation,functional enrichment,and epigenetic activity of DLGAP5.Furthermore,we evaluated the relationship between the tumor microenvironment(TME)and DLGAP5.Results:Our study identified 10 hub genes in LUAD:CDC45,KIAA0101,DLGAP5,CDT1,NCAPG,CCNB1,CDCA5,CDC20,KIF11,and AURKA.We discovered that DLGAP5 was overexpressed and associated with poor prognosis in LUAD.DLGAP5 exhibited an overall genetic variation frequency of 2%,and its DNA promoter was hypomethylated in LUAD(p<0.05).The expression of DLGAP5 in LUAD showed a positive correlation with the majority of N6-methyladenosine(m6A)-methylation genes.Additionally,DLGAP5 was primarily associated with the cell cycle in LUAD.Notably,there was a significant favorable association between DLGAP5 and CD274,CTLA4,HAVCR2,and LAG3 in LUAD.Conclusion:DLGAP5 may be a therapeutic target for LUAD,as it affects cancer cells proliferation and development through the regulation of cell-cycle checkpoints and modulation of immune cell infiltration and immune checkpoints in the TME.
文摘Substernal goiter is considered to be a dia-gnostic differential for all anterosuperior mediastinal masses.For a substernal goiter,surgical removal is sug-gested when it is large in size,with a possibility for malig-nancy or has local compression of adjacent structures.This can be performed through the neck or by the addition of a partial or complete sternotomy if necessary.A 58-year-old Chinese man from Guangdong Province had had dry cough for a year.A subsequent CT scan in our hospital suggested‘‘ectopic intrathoracic thyroid’’.Fine needle percutaneous mass biopsy also suggested‘‘ectopic intrathoracic thyroid’’.We performed a standard median sternotomy to remove the mass.It measured 13 cm 68 cm 66 cm in size,and weighed 2.8 kg.The patho-logical diagnosis confirmed a benign thyroid adenoma.The patient had no hoarseness,dyspnea or hypocalcemia and was quickly extubated in the operating room.Recently,we encountered a big substernal goiter and had performed successful resection,which is reported here for reference.
