Introduction:Human cytomegalovirus(HCMV)is reported to be involved in the occurrence of many human diseases.To further investigate the biological changes of HCMV,we analyzed the relevant factors that affect the autoph...Introduction:Human cytomegalovirus(HCMV)is reported to be involved in the occurrence of many human diseases.To further investigate the biological changes of HCMV,we analyzed the relevant factors that affect the autophagy caused by HCMV infection.Methods:Firstly,we cultured human embryonic lung fibroblasts(HELF)cells with HCMV infection,and evaluated the effects of HELF cells infected with different viruses through Enzyme-linked immunoabsorbent assay(ELISA),Real-time quantitative Polymerase Chain Reaction(RT-qPCR),Acridine orange(AO)staining and Western blotting(WB)experiments.Results:Through the above experiments,we found that the combined treatment of HCMV infection and carbamazepine,rapamycin and si-mTOR promoted the increase of interferon(IFN)-α/βexpression and protein level,and caused the increase of LC3B protein level in HELF cells.In addition,HCMV infection could also affect the biological activities of HELF cells by regulating signal pathways like the JAK/STAT.Conclusion:Autophagy induced by HCMV is affected by the changes in the biological behavior of HELF cells,especially IFN-α/βsynthesis and mTOR signal pathway.These findings might shed new light on HCMVrelated disease treatment.展开更多
基金This study was supported by Basic Public Welfare Research Project of Zhejiang Province(LY19C010006)Research Programs of Zhejiang Provincial Medicine and Health Science and Technology Plan Project(2020382731)+1 种基金Natural Science Fund of Anhui Province(Grant No.1808085MC75)Project of Sanmen County Science and Technology Planning(No.16310).
文摘Introduction:Human cytomegalovirus(HCMV)is reported to be involved in the occurrence of many human diseases.To further investigate the biological changes of HCMV,we analyzed the relevant factors that affect the autophagy caused by HCMV infection.Methods:Firstly,we cultured human embryonic lung fibroblasts(HELF)cells with HCMV infection,and evaluated the effects of HELF cells infected with different viruses through Enzyme-linked immunoabsorbent assay(ELISA),Real-time quantitative Polymerase Chain Reaction(RT-qPCR),Acridine orange(AO)staining and Western blotting(WB)experiments.Results:Through the above experiments,we found that the combined treatment of HCMV infection and carbamazepine,rapamycin and si-mTOR promoted the increase of interferon(IFN)-α/βexpression and protein level,and caused the increase of LC3B protein level in HELF cells.In addition,HCMV infection could also affect the biological activities of HELF cells by regulating signal pathways like the JAK/STAT.Conclusion:Autophagy induced by HCMV is affected by the changes in the biological behavior of HELF cells,especially IFN-α/βsynthesis and mTOR signal pathway.These findings might shed new light on HCMVrelated disease treatment.
