探讨文献计量学分析合成酚类抗氧化剂在海洋研究中的应用。方法利用China national knowledge infrastructure(CNKI)网络数据库检索以合成酚类抗氧化剂(n=135)为主题的论文,从发文趋势、主题词等方面进行文献计量学分析。结果过去8年该...探讨文献计量学分析合成酚类抗氧化剂在海洋研究中的应用。方法利用China national knowledge infrastructure(CNKI)网络数据库检索以合成酚类抗氧化剂(n=135)为主题的论文,从发文趋势、主题词等方面进行文献计量学分析。结果过去8年该领域的文献产量稳定快速增长,64.4%的论文是近5年发表的,研究主题的关联性近年来呈增加趋势,目前的研究主要集中在时空分布特征、代谢产物、优化检测方法等方面。其代谢产物的分析有助于了解结合微生物在海洋研究中的发展概况。展开更多
Activities of hexokinase(HK),pyruvate kinase(PK) and levels of HSP70 were measured to evaluate the response of Litopenaeus vannamei to rapid temperature changes under controlled laboratory conditions.Shrimps were subj...Activities of hexokinase(HK),pyruvate kinase(PK) and levels of HSP70 were measured to evaluate the response of Litopenaeus vannamei to rapid temperature changes under controlled laboratory conditions.Shrimps were subjected to a quick temperature change from 27℃ to 17℃ for the summer case(Cold temperature treatment),or from 17℃ to 27℃ for the winter case(Warm temperature treatment).After 0.5,1,3,6,12,24,48,and 72 h of exposure time,shrimps were sampled and prepared for further analysis.The results showed that the effect of acute temperature changes on activities of HK was significant.Patterns of variations of the two glycolytic enzymes suggested that enzymes in the glycolysis cycle could adjust their activities to meet the acute temperature change.The HSP70 level increased in both cold and warm temperature treatments,suggesting that the rapid temperature changes activated the process of body's self-protection.But the difference in expression peak of HSP70 might be related to the different body size and the higher thermal sensitivity to temperature increase than to temperature decrease of L.vannamei.展开更多
Octopus minor(Sasaki 1920)is an important commercial cephalopod species in China.This species has declined sharply in recent years.Hence,genetic studies of O.minor are imperative to exploit and manage the wild resourc...Octopus minor(Sasaki 1920)is an important commercial cephalopod species in China.This species has declined sharply in recent years.Hence,genetic studies of O.minor are imperative to exploit and manage the wild resource.In this study,46192 microsatellite loci were discovered in 70174 unigenes from the transcriptomic data.Among all of the simple sequence repeat(SSR)unit categories,di-nucleotide and tri-nucleotide SSRs accounted for 45.26%and 14.49%,respectively.A total of 108 SSRs were tested,of which 21 were neutral and polymorphic.Seven SSRs were selected for genetics analyses of the O.minor populations in the Bohai Sea,the Yellow Sea,and the southwest coast of the Taiwan Strait region.Significant pairwise F_(st) values were detected among the samples.The UPGMA tree based on genetic distances suggested that the sampling locations could be arranged in three clusters.These markers are evidence that the populations in this region may be structured,with samples from Penghu differing remarkably from those in northern China.The present study characterized genetic markers for population assessment,management,and conservation of O.minor.展开更多
文摘探讨文献计量学分析合成酚类抗氧化剂在海洋研究中的应用。方法利用China national knowledge infrastructure(CNKI)网络数据库检索以合成酚类抗氧化剂(n=135)为主题的论文,从发文趋势、主题词等方面进行文献计量学分析。结果过去8年该领域的文献产量稳定快速增长,64.4%的论文是近5年发表的,研究主题的关联性近年来呈增加趋势,目前的研究主要集中在时空分布特征、代谢产物、优化检测方法等方面。其代谢产物的分析有助于了解结合微生物在海洋研究中的发展概况。
基金supported by National Natural Science Foundation of China (Grant No 30571441)the Key Project of the National Scientific and Technical Supporting Programs funded by the Ministry of Science and Technology of China (Grant No 2006BAD09A07)the Major State Basic Research Development Program of China (973 Program, No 2009CB 118706)
文摘Activities of hexokinase(HK),pyruvate kinase(PK) and levels of HSP70 were measured to evaluate the response of Litopenaeus vannamei to rapid temperature changes under controlled laboratory conditions.Shrimps were subjected to a quick temperature change from 27℃ to 17℃ for the summer case(Cold temperature treatment),or from 17℃ to 27℃ for the winter case(Warm temperature treatment).After 0.5,1,3,6,12,24,48,and 72 h of exposure time,shrimps were sampled and prepared for further analysis.The results showed that the effect of acute temperature changes on activities of HK was significant.Patterns of variations of the two glycolytic enzymes suggested that enzymes in the glycolysis cycle could adjust their activities to meet the acute temperature change.The HSP70 level increased in both cold and warm temperature treatments,suggesting that the rapid temperature changes activated the process of body's self-protection.But the difference in expression peak of HSP70 might be related to the different body size and the higher thermal sensitivity to temperature increase than to temperature decrease of L.vannamei.
基金supported by the National Natural Science Foundation of China(No.31672257)the National Key Research and Development Program of China(No.2020YFD0900705).
文摘Octopus minor(Sasaki 1920)is an important commercial cephalopod species in China.This species has declined sharply in recent years.Hence,genetic studies of O.minor are imperative to exploit and manage the wild resource.In this study,46192 microsatellite loci were discovered in 70174 unigenes from the transcriptomic data.Among all of the simple sequence repeat(SSR)unit categories,di-nucleotide and tri-nucleotide SSRs accounted for 45.26%and 14.49%,respectively.A total of 108 SSRs were tested,of which 21 were neutral and polymorphic.Seven SSRs were selected for genetics analyses of the O.minor populations in the Bohai Sea,the Yellow Sea,and the southwest coast of the Taiwan Strait region.Significant pairwise F_(st) values were detected among the samples.The UPGMA tree based on genetic distances suggested that the sampling locations could be arranged in three clusters.These markers are evidence that the populations in this region may be structured,with samples from Penghu differing remarkably from those in northern China.The present study characterized genetic markers for population assessment,management,and conservation of O.minor.
